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Ehmt1 (NM_001109686) Mouse Tagged ORF Clone
CAT＃: MR226600
- TrueORF®
Ehmt1 (Myc-DDK-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 3
ORF Plasmid: tGFP
"NM_001109686" in other vectors (4)
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CNY 12,450.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9230102N17Rik; D330003E03; Eu-HMTase1; GLP; GLP1; KMT1D; mKIAA1876 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226600 representing NM_001109686
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCTGATGCTGAGCAGGCAGTTCTGGCCAAGCAAGAGACCAAGCAGGATTGCTGCATGAAAA CTGAGCTGCTAAGGGAAGATACACCTATGGCTGCTGATGAAGGTTCCACAGAGAAACAAGAAGGAGAGAC TCCCATGGCTGCAGATGGAGAAACAAATGGGTCTTGTGAAAAGAGTGGGGATCCCAGCCATCTAAATGCA CCCAAACACACTCAGGAGAACACAAGAGCTAGCCCACAGGAAGGCACCAACAGAGTGTCTCGGGTGGCAG AAAATGGGGTTTCAGAAAGAGACACAGAAGTGGGGAAGCAAAACCATGTCACAGCTGACGACTTCATGCA GACATCTGTCATTGGCAGCAATGGATATTTCTTAAATAAACCAGCCCTGCAGGGGCAGCCGTTGAGGACT CCCAACATTCTAACCTCCTCGCTTCCTGGTCATGCTGCAAAAACTCTTCCTGGAGGAGCCAGTAAATGCA GGACTCTGAGTGCACTTCCTCAGACACCAACCACAGCACCCACTGTGCCTGGGGAAGGGAGTGCAGACAC AGAGGACAGAAAGCCTACAGCCTCGGGCACTGATGTCAGGGTTCACAGGGCACGCAAGACCATGCCGAAG TCCATCTTGGGCCTGCATGCAGCCAGCAAAGACCATAGAGAAGTTCAAGACCATAAGGAACCAAAAGAGG ATATCAACAGAAACATTTCTGAATGTGGACGACAGCAGCTTTTACCAACCTTCCCAGCCCTCCACCAGTC GCTACCTCAGAATCAGTGCTACATGGCCACCACAAAGTCCCAGACAGCAGCTGCAGTATCTCGGAAGAAA AAACGAAGAATGGGAACCTATAGTTTAGTTCCCAAGAAAAAGACCAAAGTATTAAAACAGAGGACGGTGA TTGAGATGTTTAAAAGCATAACCCATTCCACTGTGGGCGCCAAGGGCGAGAAAGCCTTAGATGATAGTGC CCTGCATGTAAATGGCGAGAGCTTGGAGATGGACTCAGAAGATGAAGACTCCGATGAGCTGGAGGATGAC GAGGACCATGGAGCTGAGCAAGCGGCTGCATTTCCCACCGAGGATAGTAGGACTTCTAAAGAGAGCATGT CTGAGACTGACCGGGCTGCAAAGATGGATGGAGATTCAGAGGAGGAACAGGAGTCTCCCGACACAGGGGA GGATGAAGATGGTGGAGACGAGTCTGACCTGAGTTCTGAATCCAGTATCAAGAAGAAATTTCTCAAGAGG AGAGGGAAGACTGACAGCCCCTGGATCAAACCTGCTCGGAAAAGGAGGCGGAGAAGTAGAAAGAAGCCGA GCAGCATGCTTGGGCTGGCCAGTGGTCCGGATGTGCTGGGGACGGATGGCCTCCAGGAAGTGCCTCTCTG CAGCTGCCGAATGGAAACCCCCAAGAGCCGCGAGATCAGCACCCTGGCCAACAACCAGTGCATGGCCACT GAGAGCGTGGATCACGAATTGGGCCGGTGTACAAACAGCGTGGTCAAGTATGAGCTGATGCGCCCATCCA ACAAAGCACCGCTCTTGGTGTTATGTGAAGACCATCGGGGTCGCATGGTGAAGCACCAGTGCTGTCCTGG CTGCGGCTACTTCTGCACGGCGGGTAACTTCATGGAATGCCAGCCTGAGAGCAGCATCTCTCATCGTTTC CATAAGGACTGTGCCTCTCGAGTCAACAATGCCAGCTACTGTCCCCATTGTGGGGAAGAAGCTTCCAAGG CCAAAGAGGTGACCATAGCAAAAGCAGACACAACTTCCACAGTGACCCTAGCCCCTGGACAGGAGAAGAG CCTGGCTGCTGAAGGCAGGGCTGACACGACCACGGGCAGCATTGCTGGAGCCCCAGAGGATGAAAGATCG CAGAGCACAGCCCCCCAGGCACCAGAGTGCTTCGACCCTGCCGGACCGGCTGGGCTCGTGAGGCCGACAT CTGGCCTTTCCCAGGGCCCAGGAAAGGAAACCTTGGAAAGTGCTCTAATCGCTCTAGACTCTGAAAAACC CAAGAAACTTCGCTTCCACCCAAAGCAGCTGTACTTCTCTGCCAGGCAGGGTGAGCTGCAGAAGGTGCTT CTCATGCTGGTTGATGGAATTGATCCCAACTTCAAAATGGAGCACCAAAGTAAGCGTTCCCCATTACATG CTGCTGCGGAGGCTGGCCACGTGGACATCTGCCACATGCTGGTTCAGGCGGGTGCCAATATTGACACTTG CTCAGAGGACCAGCGGACCCCACTGATGGAGGCTGCAGAGAACAACCACTTGGATGCAGTGAAGTACCTC ATCAAGGCTGGAGCACAGGTGGATCCGAAGGACGCAGAGGGCTCCACATGTTTGCATTTGGCTGCCAAGA AAGGCCACTATGATGTGGTTCAGTATCTGCTTTCAAATGGACAGATGGATGTCAACTGCCAGGATGACGG TGGATGGACACCTATGATCTGGGCCACTGAGTACAAGCACGTGGAGCTGGTGAAGCTGCTGCTGTCTAAG GGCTCTGACATCAACATCCGGGACAACGAGGAGAACATTTGTCTGCACTGGGCAGCATTTTCAGGCTGTG TGGACATAGCTGAAATACTTCTGGCTGCCAAGTGTGACCTGCATGCTGTGAATATCCATGGAGACTCACC CCTGCACATCGCAGCCAGGGAGAATCGCTACGACTGTGTTGTCCTCTTTCTTTCTCGGGATTCAGATGTT ACTCTGAAAAACAAGGAAGGAGAGACTCCCTTGCAGTGTGCAAGTCTCAGTTCGCAGGTGTGGAGTGCAT TGCAGATGAGCAAAGCACTTCGGGACTCAGCCCCTGACAAGCCCGTTGCTGTTGAGAAGACGGTGAGCAG GGATATCGCTCGAGGGTATGAGCGCATTCCCATTCCCTGTGTCAATGCTGTGGACAGTGAGCTGTGTCCT ACCAACTATAAGTATGTCTCCCAGAACTGTGTGACATCCCCCATGAACATTGACAGGAACATCACTCATT TGCAGTACTGCGTGTGTGTAGATGACTGCTCCTCTAGCACCTGCATGTGTGGCCAGCTGAGCATGCGCTG CTGGTATGATAAGGATGGCCGACTTCTGCCTGAGTTTAACATGGCAGAACCACCCTTGATCTTCGAGTGC AATCATGCCTGCTCATGCTGGAGGAACTGCCGCAATCGTGTGGTGCAAAATGGTCTCAGGGCAAGGCTGC AGCTTTATCGGACACAGGACATGGGCTGGGGTGTGCGGTCCCTCCAGGATATCCCACTGGGCACCTTTGT CTGCGAATACGTAGGGGAGCTGATTTCGGACTCTGAAGCTGATGTTCGGGAAGAGGACTCTTACCTCTTT GATCTTGACAATAAGGATGGAGAGGTATACTGCATTGACGCTCGGTTCTATGGGAATGTCAGCCGGTTCA TAAACCACCACTGCGAACCCAACCTTGTGCCTGTGCGAGTGTTCATGTCACACCAGGACCTGCGGTTTCC CAGGATTGCCTTCTTCAGTACCCGCCTGATTCAGGCTGGGGAGCAGCTCGGGTTCGACTACGGGGAGCGC TTTTGGGACGTCAAGGGCAAGCTCTTCAGTTGCCGGTGTGGGTCTTCCAAGTGTCGGCACTCAAGCGCAG CCCTGGCCCAGAGGCAAGCCAGTGCAGCCCAGGAGCCTCAGGAGAATGGCCTTCCAGATACCAGCTCTGC AGCCGCTGCTGACCCCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226600 representing NM_001109686
Red=Cloning site Green=Tags(s) MAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNA PKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRT PNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPK SILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKK KRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDD EDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKR RGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMAT ESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRF HKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERS QSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVL LMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYL IKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSK GSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDV TLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCP TNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFEC NHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLF DLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGER FWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001109686 |
| ORF Size | 3729 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001109686.2, NP_001103156.1 |
| RefSeq Size | 4932 bp |
| RefSeq ORF | 3732 bp |
| Locus ID | 77683 |
| UniProt ID | Q5DW34 |
| MW | 136.9 kDa |
| Gene Summary | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224031 | Ehmt1 (untagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 3, (10ug) |
CNY 15,870.00 |
|
| MG226600 | Ehmt1 (tGFP-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1) transcript variant 3, (10ug) |
CNY 13,680.00 |
|
| MR226600L3 | Lenti ORF clone of Ehmt1 (Myc-DDK-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 3 |
CNY 14,350.00 |
|
| MR226600L4 | Lenti ORF clone of Ehmt1 (mGFP-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 3 |
CNY 14,350.00 |
