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Piwil4 (NM_177905) Mouse Tagged ORF Clone
CAT＃: MR224497
- TrueORF®
Piwil4 (Myc-DDK-tagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4)
ORF Plasmid: tGFP
"NM_177905" in other vectors (4)
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CNY 6,432.00
CNY 7,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9230101H05Rik; Miwi2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224497 representing NM_177905
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCTGCGCATTCTGGGGGTCCACCGGGCCCTGCCCACCCACGCTCGGGTTGCTGTTTGTAGTAACT ATCTTGGGAAGCTTGAATACTCCCAAACTCCGAGTCACAGCCACACCGTCTCTTTTGCCAAAGAGAAAAC ACTCCTGTTGAGGCTCACCAGCCCTGGAAAGCCGCTGGCACCCAGGAACATGAGTGGACGGGCCCGTGTG AGAGCGCGAGGCATCACCACTGGCCACAGTGCTAGGGAAGTGGGGCGGTCCTCAAGAGACCTCATGGTTA CATCTGCCAGTCCTGGGGACAGCGAGGCCGGAGGTGGGACAAGTGTCATTTCACAGCCATATGAACTTGG TGTCTCTTCTGGTGATGGTGGACGTACCTTCATGGAAAGAAGAGGCAAAGGCAGACAGGACTTCGAAGAG TTGGGTGTCTGTACCAGGGAAAAGTTGACCCACGTAAAAGATTGTAAAACAGGTTCTAGCGGAATACCTG TGCGGCTGGTTACAAATCTCTTTAATTTAGATTTGCCCCAGGATTGGCAGCTGTACCAGTACCATGTCAC GTACAGCCCAGATTTAGCCTCTAGGAGGCTGAGGATTGCTTTGCTTTATAATCACAGCATACTCTCAGAC AAGGCAAAAGCATTTGACGGTGCCAGCCTTTTCCTCTCAGAAAAGCTGGACCAGAAGGTCACAGAACTGA CAAGTGAAACACAAAGAGGTGAGACTATAAAGATAACGCTCACCCTAACGAGCAAGCTCTTCCCAAACTC CCCTGTGTGCATTCAGTTCTTCAATGTCATCTTCAGAAAGATCTTGAAAAACTTGAGTATGTACCAAATT GGACGGAACTTCTATAAGCCTTCAGAACCAGTGGAGATCCCTCAGTACAACAAGCTCCTATTCAATGCTG ACGTGAACTACAAAGTCCTCCGCAATGAGACCGTTCTGGACTTCATGACTGATCTCTGCCTCAGAACTGG CATGTCTTGCTTCACCGAGATGTGCCACAAACAACTAGTGGGGCTCGTTGTCCTTACCAGATACAACAAC AAAACATATCGTATCGATGACATTGACTGGTCTGTGAAGCCCACACAGGCCTTTCAGAAGCGGGACGGCT CAGAGGTCACATATGTGGATTACTACAAGCAGCAATATGATATCACCTTATCTGACCTAAATCAGCCAGT GCTTGTTAGTCTGTTGAAAAGAAAGAGAAATGATAACTCTGAGCCTCAGATGGTCCACCTGATGCCAGAG CTCTGCTTTCTCACAGGCCTGAGCAGCCAAGCAACCTCAGATTTCCGCCTGATGAAGGCAGTAGCTGAAG AGACTCGGCTCAGTCCTGTGGGAAGGCAGCAGCAGCTGGCCCGACTCGTGGATGACATCCAGAGAACCTT ACCTTCTTCTCAAGAAGTTCTCTCTCATACTTCCTTGCCTTTGTGGGCCCCAGAGCCAGGAGGACTTAGC AGTGCTATACCACTGAGCACTGTGCTTCCCTTTGCCCAGCAGCTGTTAACTGCCCTGAGCCTCTCCCCGG GAATCCCTCTGCCCCACCTCAAGCCTCCCTCCTTCCTGTTCTTGTGTCAACCTGCATTTGCTGCTGACTG GTCCAAAGATATGCGATCTTGCAAAGTTTTGAGTTCTCAGCCTTTGAATAGATGGTTGATCGTGTGCTGT AACAGGGCTGAGCACTTGATTGAAGCCTTTCTGAGCTGTCTGAGGAGAGTTGGAGGTTCCATGGGATTTA ACGTGGGCTACCCCAAAATCATAAAAGTGGACGAGACCCCAGCAGCGTTCCTTCGAGCCATCCAGGTGCA CGGCGACCCCGATGTTCAGTTGGTGATGTGCATTCTGCCTTCTAATCAGAAGAACTATTACGACTCCATT AAAAAGTATTTGAGCTCTGACTGCCCAGTGCCAAGCCAGTGTGTGCTGACCCGGACCTTGAATAAGCAGG GAACGATGCTGAGTGTGGCCACCAAGATCGCCATGCAGATGACCTGCAAACTTGGCGGAGAGCTGTGGTC TGTGGAGATCCCATTGAAGTCCCTGATGGTCGTGGGTATTGATATCTGCAGAGATGCCCTCAACAAGAAT GTGGTGGTCGTCGGGTTTGTAGCCAGCATTAATTCCAGGATCACCAGGTGGTTTTCCCGCTGTGTCCTTC AGAGAACAGCGGCTGATATTGCAGATTGCCTCAAAGTCTGCATGACTGGTGCTCTCAACCGGTGGTACAG ACACAACCATGACTTGCCGGCACGGATAGTCGTGTACCGGGACGGTGTAGGCAATGGCCAGCTAAAGGCA GTTTTGGAATATGAAGTCCCACAGCTACTGAAAAGTGTAACAGAGTGCGGCTCGGATGCCAGGTATGACT TCTACCTGATCAGCCAGACTGCTAACCGGGGGACTGTTAGTCCCACCCACTACAACGTTATCTATGATGA CAATGCCTTGAAGCCTGACCACATGCAGCGACTGACCTTCAAACTGTGCCATCTCTACTACAACTGGCAG GGCTTAATCAGTGTCCCCGCACCATGCCAATATGCACACAAGCTGACCTTCCTGGTGGCACAAAGTGTCC ACAAGGAACCAAGTCTGGAATTAGCCAATAATCTTTTCTACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224497 representing NM_177905
Red=Cloning site Green=Tags(s) MRLRILGVHRALPTHARVAVCSNYLGKLEYSQTPSHSHTVSFAKEKTLLLRLTSPGKPLAPRNMSGRARV RARGITTGHSAREVGRSSRDLMVTSASPGDSEAGGGTSVISQPYELGVSSGDGGRTFMERRGKGRQDFEE LGVCTREKLTHVKDCKTGSSGIPVRLVTNLFNLDLPQDWQLYQYHVTYSPDLASRRLRIALLYNHSILSD KAKAFDGASLFLSEKLDQKVTELTSETQRGETIKITLTLTSKLFPNSPVCIQFFNVIFRKILKNLSMYQI GRNFYKPSEPVEIPQYNKLLFNADVNYKVLRNETVLDFMTDLCLRTGMSCFTEMCHKQLVGLVVLTRYNN KTYRIDDIDWSVKPTQAFQKRDGSEVTYVDYYKQQYDITLSDLNQPVLVSLLKRKRNDNSEPQMVHLMPE LCFLTGLSSQATSDFRLMKAVAEETRLSPVGRQQQLARLVDDIQRTLPSSQEVLSHTSLPLWAPEPGGLS SAIPLSTVLPFAQQLLTALSLSPGIPLPHLKPPSFLFLCQPAFAADWSKDMRSCKVLSSQPLNRWLIVCC NRAEHLIEAFLSCLRRVGGSMGFNVGYPKIIKVDETPAAFLRAIQVHGDPDVQLVMCILPSNQKNYYDSI KKYLSSDCPVPSQCVLTRTLNKQGTMLSVATKIAMQMTCKLGGELWSVEIPLKSLMVVGIDICRDALNKN VVVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNRWYRHNHDLPARIVVYRDGVGNGQLKA VLEYEVPQLLKSVTECGSDARYDFYLISQTANRGTVSPTHYNVIYDDNALKPDHMQRLTFKLCHLYYNWQ GLISVPAPCQYAHKLTFLVAQSVHKEPSLELANNLFYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177905 |
| ORF Size | 2634 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177905.3, NP_808573.2 |
| RefSeq Size | 2637 bp |
| RefSeq ORF | 2637 bp |
| Locus ID | 330890 |
| UniProt ID | Q8CGT6 |
| MW | 99.1 kDa |
| Gene Summary | Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:17395546, PubMed:18381894, PubMed:18922463, PubMed:26669262, PubMed:22020280). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:17395546, PubMed:18381894, PubMed:18922463, PubMed:26669262, PubMed:22020280). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (PubMed:17395546, PubMed:18381894, PubMed:18922463, PubMed:26669262, PubMed:22020280). The piRNA process acts upstream of known mediators of DNA methylation (PubMed:17395546, PubMed:18381894, PubMed:18922463, PubMed:26669262, PubMed:22020280). Does not show endonuclease activity (PubMed:22020280). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (PubMed:22020280). In addition to its role in germline, PIWIL4 also plays a role in the regulation of somatic cells activities. Plays a role in pancreatic beta cell function and insulin secretion (By similarity). Involved in maintaining cell morphology and functional integrity of retinal epithelial through Akt/GSK3alpha/beta signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222360 | Piwil4 (untagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4), (10ug) |
CNY 11,210.00 |
|
| MG224497 | Piwil4 (tGFP-tagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4), (10ug) |
CNY 8,460.00 |
|
| MR224497L3 | Lenti ORF clone of Piwil4 (Myc-DDK-tagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4) |
CNY 9,600.00 |
|
| MR224497L4 | Lenti ORF clone of Piwil4 (mGFP-tagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4) |
CNY 9,600.00 |
