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Robo2 (NM_175549) Mouse Tagged ORF Clone
CAT＃: MR223981
- TrueORF®
Robo2 (Myc-DDK-tagged) - Mouse roundabout homolog 2 (Drosophila) (Robo2)
ORF Plasmid: tGFP
"NM_175549" in other vectors (4)
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CNY 10,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2600013A04Rik; 9430089E08Rik; BB097918; D230004I22Rik; mKIAA1568 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223981 representing NM_175549
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCTCTGATGTTTACACTATTATTGCTCTTTGGATTTCTCTGCATTCAGATTGATGGATCTCGTC TTCGTCAAGAAGACTTTCCCCCCAGGATCGTAGAACACCCTTCTGATGTCATCGTCTCCAAGGGAGAGCC CACCACTCTGAACTGTAAAGCAGAGGGCCGACCCACCCCCACCATTGAATGGTACAAGGATGGTGAGAGG GTGGAGACAGACAAGGATGATCCCAGGTCCCACAGAATGCTTCTGCCCAGCGGATCTTTATTCTTTTTGC GAATTGTTCATGGGCGCAGAAGTAAACCGGACGAAGGGAGTTACGTTTGTGTTGCAAGGAACTATCTTGG TGAAGCAGTGAGTCGAAATGCATCTCTGGAAGTGGCATTATTGCGAGATGACTTCCGGCAAAACCCCACA GATGTGGTAGTCGCAGCTGGAGAGCCTGCAATCTTGGAGTGCCAGCCACCACGGGGACACCCAGAACCAA CCATCTACTGGAAAAAGGACAAAGTCCGAATTGATGACAAGGAAGAGAGAATAAGTATCCGTGGTGGGAA GCTGATGATCTCTAATACTAGGAAAAGCGATGCTGGCATGTACACCTGTGTGGGAACCAATATGGTGGGA GAAAGGGACAGCGACCCTGCAGAGCTCACTGTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACC AGGTGGTGCTAGAGGAAGAAGCTGTAGAATTCCGTTGTCAGGTCCAAGGAGATCCCCAGCCAACGGTGAG GTGGAAAAAAGATGATGCAGACTTGCCGAGAGGAAGGTATGATATCAAAGATGACTACACGCTGAGAATT AAAAAGGCCATGAGTACTGATGAAGGTACCTATGTGTGTATTGCTGAGAATCGGGTGGGAAAAGTGGAAG CCTCTGCTACCCTCACTGTCCGAGCTCCTCCACAGTTTGTGGTTAGGCCAAGAGATCAGATCGTTGCTCA AGGCCGAACAGTGACATTCCCCTGTGAAACTAAAGGAAACCCACAGCCAGCTGTTTTTTGGCAGAAAGAA GGCAGCCAGAACCTACTTTTCCCGAATCAACCTCAGCAGCCCAACAGCCGATGTTCAGTGTCGCCCACGG GGGACCTCACCATCACCAACATCCAGCGTTCAGATGCGGGTTACTACATCTGCCAGGCCCTAACCGTGGC AGGAAGCATTTTAGCTAAAGCACAGTTGGAAGTTACTGACGTTTTGACAGATAGACCTCCACCCATAATC TTGCAAGGACCAATAAACCAAACACTTGCAGTAGACGGTACAGCATTGTTGAAGTGTAAAGCCACTGGTG AGCCTCTGCCTGTAATTAGCTGGCTAAAGGAGGGCTTTACTTTTCTGGGGAGAGATCCAAGAGCCACGAT CCAAGACCAAGGAACACTGCAGATTAAGAATTTACGGATATCTGATACTGGCACTTATACTTGTGTGGCT ACAAGTTCCAGTGGAGAGACTTCCTGGAGTGCAGTGCTGGATGTAACAGAATCTGGAGCAACAATCAGTA AAAATTATGATATGAATGACCTCCCGGGACCACCATCCAAACCTCAGGTCACTGATGTTTCTAAGAACAG TGTCACCTTATCCTGGCAGCCAGGTACACCTGGCGTTCTTCCTGCAAGCGCGTATATCATTGAGGCTTTC AGCCAATCGGTGAGCAATAGCTGGCAGACAGTGGCAAACCATGTTAAGACAACTCTGTATACAGTAAGGG GGCTGAGGCCCAACACAATCTACTTGTTTATGGTCAGAGCGATCAACCCACAAGGTCTCAGTGATCCAAG TCCTATGTCGGATCCTGTACGCACACAAGATATCAGCCCCCCAGCACAAGGAGTGGACCACAGACAGGTG CAGAAGGAATTAGGTGATGTCGTTGTTCGTCTCCATAATCCAGTTGTCCTGACACCTACAACTGTTCAAG TCACATGGACGGTGGACCGACAACCCCAGTTTATTCAGGGCTACAGAGTGATGTACCGTCAGACTTCGGG ACTACAAGCCTCAACTGTGTGGCAGAATCTAGACGCCAAAGTCCCGACTGAGAGGAGTGCTGTCCTTGTG AATTTGAAAAAGGGGGTGACTTATGAAATTAAAGTCCGGCCGTATTTTAACGAGTTCCAAGGAATGGACA GTGAATCGAAAACAGTCCGAACCACTGAGGAAGCCCCAAGTGCCCCTCCCCAGTCTGTCACTGTGCTGAC AGTTGGAAGTCACAACAGCACAAGCATCAGTGTTTCCTGGGATCCTCCACCAGCCGACCACCAGAATGGA ATTATTCAGGAATATAAGATCTGGTGTCTGGGAAACGAAACGCGATTCCATATCAATAAAACGGTGGATG CAGCCATTCGCTCTGTAGTAATAGGTGGCTTGTTCCCTGGAATTCAGTACCGGGTAGAAGTGGCAGCTAG CACAAGTGCAGGGGTTGGAGTAAAAAGTGAACCACAGCCGATAATAATTGGGGGACGTAACGAAGTTGTC ATTACTGAAAACAATAACAGCATCACTGAGCAAATCACGGATGTCGTGAAGCAACCGGCATTTATAGCTG GCATTGGTGGTGCCTGCTGGGTAATTCTGATGGGTTTTAGCATCTGGTTGTACTGGAGAAGAAAGAAGAG AAAGGGACTCAGCAATTATGCTGTAACATTTCAAAGAGGAGATGGAGGACTAATGAGCAATGGGAGCCGT CCAGGTCTTCTAAATGCTGGCGATCCCAATTACCCATGGCTTGCTGATTCTTGGCCAGCCACGAGTTTGC CAGTGAACAATAGCAATAGTGGCCCAAATGAAATTGGAAATTTTGGGCGTGGAGATGTGCTGCCTCCGGT GCCAGGCCAAGGGGATAAAACAGCGACCATGCTCTCGGATGGAGCCATTTATAGCAGCATTGACTTCACT ACCAAAACCACTTACAACAGTTCCAGCCAAATAACACAGGCCACCCCATATGCCACTACACAAATCCTGC ATTCAAACAGCATCCACGAACTGGCAGTTGATCTTCCTGATCCACAGTGGAAAAGCTCAGTTCAACAGAA GACAGACCTCATGGGATTTGGTTATTCGCTACCTGATCAGAACAAGGGGAACAACGCCTTACTTTACATC CCTGACTACCGATTGGCTGAGGGATTGTCTAATAGAATGCCACACAACCAGTCACAGGATTTCAGCACCA CCAGCTCTCACAACAGCTCAGAAAGGAGTGGCAGTCTCTCAGGTGGGAAAGGTGGAAAAAAGAAGAAAAC TAAAAATTCTTCGAAAGCGCAGAAAAACAACGGATCCACTTGGGCTAATGTCCCTCTACCTCCTCCTCCC GTCCAGCCCCTTCCTGGTACAGAGCTGGGCCACTATGCTGCGGAACAAGAAAATGGCTATGACAGTGACA GCTGGTGCCCACCATTACCGGTGCAAACATACTTGCATCAGGGTATGGAAGATGAGCTGGAAGAAGACGA AGATCGGGTCCCAACACCTCCTGTTCGCGGCGTGGCCTCTTCACCAGCTATCTCTTTTGGACAGCAGTCC ACTGCCACTCTTACTCCATCCCCACGGGAAGAGATGCAACCCATGCTGCAAGCTCACTTGGATGAGTTGA CAAGGGCCTATCAGTTTGATATAGCAAAACAAACATGGCACATTCAAAGCAATACCCCACCTCCACAACC CCCAGCTCCGCCATTAGGTTACGTGTCCGGAGCCTTGATTTCTGATTTGGAGACAGATGTTCCAGATGAG GATGCTGATGATGAAGAGGAACCATTAGAAATTCCCAGGCCCCTCAGAGCACTAGACCAGACACCTGGAT CCAGTATGGACAATCTAGACAGCTCTGTCACAGGAAAAGCCTTTAGCTCCTCTCAAAGGCAGCGGCCCAC CAGCCCATTTTCTACGGACAGTAACACCAGTGCTGCCCAGAATCAAAGCCAGAGGCCTCGGCCCACGAAA AAACATAAGGGAGGACGGATGGACCCACAGCCAGTGTTACCTCATCGGAGGGAAGGGATGCCAGATGATC TTCCCCCACCACCAGATCCCCCACCAGGTCAGGGTTTAAGGCAGCAGATAGGCCTGAGCCAACACTCTGG TAACGTGGAAAACTCAACAGAGAGAAAAGGAAGCTCTCTAGAGAGACAACAAGCAGCCAACTTAGAAGAC ACAAAGAGCTCATTGGATTGTCCAGCTAAAACTGTTCTAGAGTGGCAGCGTCAAACCCAGGACTGGATAA ACTCTACAGAACGCCAAGAAGAAACTCGGAAAGCCCCACACAAACAAGGGGTTGGATCAGAGGAGTCCCT GGTGCCCTACAGCAAGCCCAGCTTCCCGTCTCCTGGCGGGCACAGCTCATCAGGAACATCTTCCTCTAAA GGGTCCACTGGCCCTCGGAAGGCTGACGTGCTGAGAGGCAGCCACCAGAGGAATGCCAATGACCTTCTCG ACATTGGATATGTGGGCTCAAATAGTCAAGGACAGTTTACAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223981 representing NM_175549
Red=Cloning site Green=Tags(s) MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGER VETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPT DVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVG ERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRI KKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKE GSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPII LQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVA TSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAF SQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQV QKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLV NLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNG IIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVV ITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSR PGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFT TKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYI PDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPP VQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQS TATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDE DADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTK KHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLED TKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSK GSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175549 |
| ORF Size | 4524 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175549.5 |
| RefSeq Size | 8047 bp |
| RefSeq ORF | 4527 bp |
| Locus ID | 268902 |
| MW | 165.8 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224626 | Robo2 (untagged) - Mouse roundabout homolog 2 (Drosophila) (Robo2), (10ug) |
CNY 19,290.00 |
|
| MG223981 | Robo2 (tGFP-tagged) - Mouse roundabout homolog 2 (Drosophila) (Robo2), (10ug) |
CNY 14,630.00 |
|
| MR223981L3 | Lenti ORF clone of Robo2 (Myc-DDK-tagged) - Mouse roundabout homolog 2 (Drosophila) (Robo2) |
CNY 12,720.00 |
|
| MR223981L4 | Lenti ORF clone of Robo2 (mGFP-tagged) - Mouse roundabout homolog 2 (Drosophila) (Robo2) |
CNY 15,200.00 |
