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Ldb1 (NM_010697) Mouse Tagged ORF Clone
CAT＃: MR223855
- TrueORF®
Ldb1 (Myc-DDK-tagged) - Mouse LIM domain binding 1 (Ldb1), transcript variant 3
ORF Plasmid: tGFP
"NM_010697" in other vectors (4)
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CNY 3,710.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CLIM2; NLI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223855 representing NM_010697
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGATCGGGATGTGGGCCCAACTCCCATGTACCCACCTACATACCTGGAGCCTGGGATCGGGAGGC ACACACCATATGGTAACCAAACCGACTATAGAATATTTGAGCTTAACAAACGGCTACAGAACTGGACAGA GGAGTGTGACAATCTCTGGTGGGATGCTTTCACAACTGAGTTCTTTGAAGATGACGCCATGCTGACCATC ACTTTCTGCTTGGAGGATGGACCAAAGAGATATACCATTGGCCGGACCCTGATACCACGCTACTTCCGAA GCATTTTTGAGGGGGGTGCCACAGAGCTGTACTACGTGCTCAAGCACCCCAAGGAGGCATTCCACAGCAA CTTCGTGTCCCTCGACTGTGACCAGGGCAGCATGGTGACCCAGCACGGCAAACCCATGTTTACCCAGGTG TGTGTGGAAGGCCGGTTGTACCTGGAGTTCATGTTTGACGACATGATGCGGATAAAGACGTGGCACTTCA GCATCCGGCAACACAGAGAGCTCATCCCCAGAAGTATCCTGGCCATGCACGCCCAGGACCCCCAGATGCT GGATCAGCTGTCCAAAAACATTACCCGGTGTGGGCTGTCCAATTCCACTCTCAACTACCTCCGACTCTGT GTGATACTAGAGCCCATGCAGGAACTTATGTCCCGCCACAAGACCTACAGCCTCAGCCCCCGAGACTGCC TCAAGACCTGCCTCTTCCAGAAGTGGCAGCGAATGGTAGCGCCTCCCGCGGAGCCCGCACGACAGCAGCC CAGCAAACGGAGGAAACGGAAGATGTCAGGGGGTAGCACCATGAGCTCGGGGGGTGGCAACACCAACAAC AGCAACAGCAAGAAGAAGAGCCCAGCCAGCACCTTCGCTCTCTCCAGCCAGGTACCTGATGTGATGGTGG TGGGGGAGCCCACCCTGATGGGCGGGGAGTTCGGGGACGAGGACGAGAGGCTCATCACCCGGCTGGAGAA CACCCAGTTTGACGCGGCCAACGGCATTGACGACGAGGACAGCTTTAACAACTCCCCCGCACTGGGCGCC AACAGCCCCTGGAACAGCAAGCCTCCATCCAGCCAAGAGAGCAAATCGGAGAATCCCACGTCACAGGCTT CCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223855 representing NM_010697
Red=Cloning site Green=Tags(s) MLDRDVGPTPMYPPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTI TFCLEDGPKRYTIGRTLIPRYFRSIFEGGATELYYVLKHPKEAFHSNFVSLDCDQGSMVTQHGKPMFTQV CVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMHAQDPQMLDQLSKNITRCGLSNSTLNYLRLC VILEPMQELMSRHKTYSLSPRDCLKTCLFQKWQRMVAPPAEPARQQPSKRRKRKMSGGSTMSSGGGNTNN SNSKKKSPASTFALSSQVPDVMVVGEPTLMGGEFGDEDERLITRLENTQFDAANGIDDEDSFNNSPALGA NSPWNSKPPSSQESKSENPTSQASQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010697 |
| ORF Size | 1125 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010697.2 |
| RefSeq Size | 2295 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 16825 |
| UniProt ID | P70662 |
| MW | 43.2 kDa |
| Gene Summary | Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208871 | Ldb1 (untagged) - Mouse LIM domain binding 1 (Ldb1), transcript variant 3, (10ug) |
CNY 3,990.00 |
|
| MG223855 | Ldb1 (tGFP-tagged) - Mouse LIM domain binding 1 (Ldb1) transcript variant 3, (10ug) |
CNY 4,090.00 |
|
| MR223855L3 | Lenti ORF clone of Ldb1 (Myc-DDK-tagged) - Mouse LIM domain binding 1 (Ldb1), transcript variant 3 |
CNY 5,610.00 |
|
| MR223855L4 | Lenti ORF clone of Ldb1 (mGFP-tagged) - Mouse LIM domain binding 1 (Ldb1), transcript variant 3 |
CNY 5,610.00 |
