Dock2 (NM_033374) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI662014; AW122239; CED-5; Hch; MBC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223405 representing NM_033374 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCTGGCGCAAAACTGACAAGGAGCGTCACGGAGTGGCTATCTACAACTTCCAAGGCAGTGAAG CCCAGCATCTCACGCTACAGATTGGCGATGTGGTACGAATACAGGAGACCTGTGGAGACTGGTACAGAGG GTACCTCATAAAGCATAAACTGTCACAGGGCATTTTCCCTACATCCTTTATCCATCTCAAGGAAGTGACA GTGGAGAAGAGAAGGAACATAGAGAACATCATTCCTGCAGAAATCCCTCTGGCACAAGAAGTGACAACCA CGCTCTGGGAGTGGGGAAGCATCTGGAAGCAGCTCTATGTGGCCAGCAAAAAGGAACGCTTCCTCCAAGT GCAGTCCATGATGTACGACCTGATGGAATGGCGCTCTCAGCTCCTCTCAGGAACGCTACCCAAGGATGAG CTGAAGGAACTGAAGCAGAAAGTCACATCGAAGATCGACTATGGCAACAAAATCCTTGAGCTCGATCTCA TTGTCAGAGATGAAGATGGAAACATACTGGACCCTGATAAGACCAGCGTCATCAGCTTGTTCCATGCTCA CGAGGAGGCAACTTACAAAATCACAGAGCGCATCAAAGAAGAAATGTCAAAAGACCAGCCAGATTATGGG GTTTATTCTCGGATCTCCTCATCCCCCACCCACAGCCTCTACGTCTTTGTGAGAAACTTTGTGTGCCGAA TTGGGGAGGATGCTGAGCTCTTCATGTCTCTCTATGACCCTCACAAGCAAACGGTCATCAGTGAGAACTA TCTGGTGCGATGGGGCAGCAAAGGATTCCCAAAGGAAATTGAGATGCTCAATAACCTGAAGGTAGTCTTC ACGGATCTTGGAAATAAAGACCTCAACAGGGATAAGATTTTCTTGATCTGTCAAATAGTCCGAATTGGGA AGATGGATCTTAAGGACATTAATGCCAAGAAGTGCACCCAGGGGCTGAGGAGACCTTTTGGGGTGGCAGT TATGGACATCACCGACATCATCAAGGGCAAGGCAGAGAGCGACGAAGAGAAGCAACATTTTATTCCATTT CACCCGGTCTCAGCTGAGAACGATTTCCTTCACAGCCTTCTGGGCAAAGTCATAGCTTCCAAGGGAGACA GTGGAGGGCAAGGCCTTTGGGTGACCATGAAGATGCTGGTGGGGGACATCATTCAGATCCGTAAAGACTA CCCACACTTGGTGGACAGGACCACTGTGGTAGCCCGAAAGCTGGGCTTCCCAGAGATCATCATGCCAGGG GATGTCAGGAACGACATCTACATCACTCTCTTACAAGGTGACTTTGACAAGTACACCAAGACCACACAGC GGAACGTGGAAGTGATCATGTGTGTGTGCACAGAGGATGGCAAAGTGCTACCTAATGCAATTTGTGTGGG AGCGGGGGATAAGGCCATGAACGAGTACCACTCAGTCGTCTACTACCAAGTCAAACAGCCCCGATGGATG GAAACAGTCAAGGTGGCTGTCCCTATTGAAGATATGCAGAGGATCCATTTGAGGTTCATGTTTCGACACC GCTCATCACTAGAATCTAAAGATAAAGGAGAAAAGAACTTTGCCATGTCCTACGTGAAACTCATGAAAGA AGATGGAACCACTCTGCATGATGGATACCACGAGTTGGTGGTTCTCAAGGGAGACAGCAAGAAAATGGAA GATGCCAGCGCTTACCTGACACTTCCTTCTTATCGACACCCCGTGGAAAACAAGGGAGCTACGCTGAGCC GGAGCTCCAGCAGTGTTGGGGGCCTTTCTGTCAGCTCCCGGGATGTGTTCTCCATTTCCACGCTAGTGTG CTCCACAAAGCTGACCCAGAATGTGGGTTTGCTTGGCTTGCTGAAGTGGCGTATGAAGCCACAACTGCTC CAGGAGAATCTAGAGAAGTTGAAGATTGTGGATGGCGAGGAAGTAGTGAAGTTCCTCCAGGACACTCTGG ATGCCCTCTTCAACATCATGATGGAACACTCTCAGAGTAACGAGTATGACATCCTCGTCTTTGATGCTTT GATCTATATAATAGGACTCATTGCAGACCGGAAATTCCAGCATTTTAACACCGTATTGGAGGCTTACATC CAACAGCATTTCAGTGCTACCTTGGCCTACAAGAAACTGATGACGGTGCTGAAGACTTACTTGGATACCT CCAGCAGGGGGGAGCAGTGCGAGCCCATCCTCAGAACGCTCAAAGCCTTAGAATACGTGTTCAAGTTCAT TGTTCGGTCGAGGACATTATTCTCACAGCTTTACGAAGGCAAAGAGCAGATGGAGTTTGAAGAATCCATG AGACGGCTCTTCGAATCCATCAACAACCTGATGAAAAGTCAGTACAAGACCACCATCCTATTGCAGGTGG CGGCTTTGAAATACATCCCTTCAGTCCTTCACGATGTGGAAACAGTCTTTGATGCCAAGTTGCTCAGCCA GCTACTGTATGAGTTCTACACCTGCATCCCTCCCGTGAAGCTGCAGAAGCAGAAAGTTCAGTCCATGAAT GAGATCGTGCAGAGCAACCTCTTCAAAAAGCAAGAATGCCGGGACATTCTGCTTCCTGTCATCACCAAAG AGCTGAAGGAGCTGCTGGAGCAGAGGGACGATGGGCAGCACCAGGCTGAGAAGAAGCACTGTGTGGAACT TCTCAACAGCATCTTGGAGGTCCTCAGCTGTCAGGATGCGGCCTTCACTTATGACCACATCCAAGAGATC ATGGTCCAGCTGCTTCGCACAGTGAACCGGACAGTCATCACGATGGGCCGAGATCACGCTTTGATTAGTC ACTTTGTGGCGTGTATGACAGCTATCTTAGACCAGATGGGCGACCAACACTATTCATTTTACATTGAGAC CTTCCAGACCAGCTCAGACCTTGTGGACTTCTTGATGGAGACATTCATCATGTTCAAGGACCTCATTGGG AAGAATGTGTATCCTGGGGACTGGATGGCCATGAGCATGGTCCAGAACCGGGTCTTCCTGAGAGCCATCA ACAAGTTTGCAGAAACCATGAACCAGAAGTTTCTGGAACACACAAGCTTTGAGTTCCAGCTGTGGAACAA CTATTTTCATCTGGCAGTTGCCTTTATCACTCAGGACTCTCTGCAGCTGGAGCAGTTCACACACGCTAAG TACAACAAAATCCTGAATAAGTACGGGGACATGAGACGGCTCATAGGATTCTCCATCCGTGATATGTGGT ACAAGCTTGGCCAGAACAAAATCTGCTTCATCCCGGGCATGGTGGGACCTATATTAGAGATGACACTTAT CCCTGAGGCTGAGCTCAGGAAAGCCACCATCCCAATCTTCTTCGACATGATGCTGTGTGAATATCAAAGG ACTGGGGCTTTCAAAAAGTTTGAAAATGAAATCATCCTGAAGCTGGACCATGAGGTGGAAGGAGGCCGGG GGGATGAGCAGTACATGCAACTGCTGGAGTCCATCCTGATGGAGTGCACAGCGGAACACCCAACCATTGC CAAGTCGGTGGAGAACTTTGTGAGCTTGGTCAAAGGGCTGCTGGAGAAGTTGCTAGATTACCGGGGCGTG ATGACAGATGAGAGCAAAGACAACCGTATGAGCTGCACAGTGAACTTGCTGAATTTCTACAAAGATAACA ACCGGGAAGAGATGTACATAAGATACCTGTATAAACTGCGTGACCTTCACTTGGACTGTGAAAACTACAC AGAGGCTGCCTACACACTCCTCCTCCACACCTGGCTTCTCAAGTGGTCAGATGAGCAGTGTGCATCCCAG GTCATGCAGACAGGCCAGCAGCACCCTCAGACCCACCGGCAGCTGAAGGAGACACTCTATGAGACCATTA TAGGCTACTTTGACAAAGGAAAGATGTGGGAAGAGGCCATCAGCCTGTGCAAGGAACTGGCGGAACAATA TGAGATGGAGATCTTTGACTACGAGCTACTCAGCCAGAACCTGACCCAGCAGGCCAAATTCTATGAAAAC ATCATGAAAATCCTCAGAACCAAACCAGACTACTTTGCTGTTGGATACTATGGCCAGGGATTCCCCTCCT TTCTGCGGAACAAAGTGTTCATCTACCGAGGCAAGGAATATGAGCGAAGAGAAGACTTCCAGATGCAGCT TTTGAGCCAGTTCCCCAACGCAGAAAAGATGAACACAACTTCTGCCCCAGGAGACGATGTGAGGAATGCC CCAGGCCAGTACATCCAGTGTTTCACTGTGCAGCCTGTTCTGGATGAACACCCCAGGTTCAAGAACAAAC CGGTGCCTGACCAGATCATAAACTTTTACAAGTCTAATTATGTGCAAAAGTTCCACTACTCCAGGCCTGT GCGCAGGGGCAAGGTAGACCCAGAGAACGAGTTTGCTTCCATGTGGATCGAGAGGACTTCCTTCCTGACG GCCTACAAGCTGCCTGGCATCCTGCGCTGGTTTGAGGTAGTTCACATGTCTCAGACCACAATTAGTCCTC TGGAGAATGCCATCGAGACTATGTCCACAGTCAACGAGAAGATCCTGATGATGATAAACCAGTACCAGAG TGATGAAAGCCTCCCCATCAACCCTCTCTCCATGCTCCTCAATGGGATCGTGGACCCTGCTGTCATGGGA GGCTTCGCCAAGTATGAGAAGGCCTTCTTCACTGAAGAGTATAGCAGGGAGCACCCGGAGGACCAGGACA AGCTGAGCCATCTCAAGGACCTGATTGCATGGCAGATCCCTTTCCTGGGAGCTGGGATTAAGATCCACGA GAAAAGGGTGTCAGACAACCTGCGCCCCTTCCATGACCGGATGGAGGAGTGCTTCAAGAACCTGAAAATG AAGGTGGAAAAGGAGTATGGTGTCCGAGAGATGCCTGACTTCGAAGACAGGAGAGTGGGCCGCCCCAGGT CCATGCTGCGTTCCTACAGGCAGATGTCCGTCATCTCTCTGGCCTCCATGCATTCTGACTGCAGCACTCC CAGCAAAGTCCCCGCAGAAAGTTTTGACCTAGAGTCAGCCCCACCCAAGACTCCCAAAGTGGAGGAGGAG CCCATCTCCCCAGGGAGCACTCTGCCAGAGGTCAAGCTGCGGCGGTCCAAGAAAAGGACCAAGAGGAGCA GCGTGGTGTTTGCAGACGAGAAGGCAGCAACGGAGTCAGACCTGAAGCGGCTTTCTAGAAAGCAAGAGTT CATGAGTGACACCAACCTCTCAGAGCATGCAGCCATCCCTGCCAGGGTGTCTATCCTCTCTCAGATGAGT TTTGCCAGCCAGTCCATGCCCACCATCCCAGCCCTCACACTCTCCGTGGCAGGCGTGCCTGGGTTGGATG AGGCCAACACATCTCCCCGCCTGAGTCAGACCTTCTTCCAAGTCTCAGATGGTGACAAGAAGACCCTGAA AAAGAAGAAAGTCAATCAATTCTTCAAGACCATGCTGGCTAGCAAGTCGAGTGAAGAAAGCAAGCAGATC CCAGACTTCCTGTCCACCAACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223405 representing NM_033374 Red=Cloning site Green=Tags(s) MAPWRKTDKERHGVAIYNFQGSEAQHLTLQIGDVVRIQETCGDWYRGYLIKHKLSQGIFPTSFIHLKEVT VEKRRNIENIIPAEIPLAQEVTTTLWEWGSIWKQLYVASKKERFLQVQSMMYDLMEWRSQLLSGTLPKDE LKELKQKVTSKIDYGNKILELDLIVRDEDGNILDPDKTSVISLFHAHEEATYKITERIKEEMSKDQPDYG VYSRISSSPTHSLYVFVRNFVCRIGEDAELFMSLYDPHKQTVISENYLVRWGSKGFPKEIEMLNNLKVVF TDLGNKDLNRDKIFLICQIVRIGKMDLKDINAKKCTQGLRRPFGVAVMDITDIIKGKAESDEEKQHFIPF HPVSAENDFLHSLLGKVIASKGDSGGQGLWVTMKMLVGDIIQIRKDYPHLVDRTTVVARKLGFPEIIMPG DVRNDIYITLLQGDFDKYTKTTQRNVEVIMCVCTEDGKVLPNAICVGAGDKAMNEYHSVVYYQVKQPRWM ETVKVAVPIEDMQRIHLRFMFRHRSSLESKDKGEKNFAMSYVKLMKEDGTTLHDGYHELVVLKGDSKKME DASAYLTLPSYRHPVENKGATLSRSSSSVGGLSVSSRDVFSISTLVCSTKLTQNVGLLGLLKWRMKPQLL QENLEKLKIVDGEEVVKFLQDTLDALFNIMMEHSQSNEYDILVFDALIYIIGLIADRKFQHFNTVLEAYI QQHFSATLAYKKLMTVLKTYLDTSSRGEQCEPILRTLKALEYVFKFIVRSRTLFSQLYEGKEQMEFEESM RRLFESINNLMKSQYKTTILLQVAALKYIPSVLHDVETVFDAKLLSQLLYEFYTCIPPVKLQKQKVQSMN EIVQSNLFKKQECRDILLPVITKELKELLEQRDDGQHQAEKKHCVELLNSILEVLSCQDAAFTYDHIQEI MVQLLRTVNRTVITMGRDHALISHFVACMTAILDQMGDQHYSFYIETFQTSSDLVDFLMETFIMFKDLIG KNVYPGDWMAMSMVQNRVFLRAINKFAETMNQKFLEHTSFEFQLWNNYFHLAVAFITQDSLQLEQFTHAK YNKILNKYGDMRRLIGFSIRDMWYKLGQNKICFIPGMVGPILEMTLIPEAELRKATIPIFFDMMLCEYQR TGAFKKFENEIILKLDHEVEGGRGDEQYMQLLESILMECTAEHPTIAKSVENFVSLVKGLLEKLLDYRGV MTDESKDNRMSCTVNLLNFYKDNNREEMYIRYLYKLRDLHLDCENYTEAAYTLLLHTWLLKWSDEQCASQ VMQTGQQHPQTHRQLKETLYETIIGYFDKGKMWEEAISLCKELAEQYEMEIFDYELLSQNLTQQAKFYEN IMKILRTKPDYFAVGYYGQGFPSFLRNKVFIYRGKEYERREDFQMQLLSQFPNAEKMNTTSAPGDDVRNA PGQYIQCFTVQPVLDEHPRFKNKPVPDQIINFYKSNYVQKFHYSRPVRRGKVDPENEFASMWIERTSFLT AYKLPGILRWFEVVHMSQTTISPLENAIETMSTVNEKILMMINQYQSDESLPINPLSMLLNGIVDPAVMG GFAKYEKAFFTEEYSREHPEDQDKLSHLKDLIAWQIPFLGAGIKIHEKRVSDNLRPFHDRMEECFKNLKM KVEKEYGVREMPDFEDRRVGRPRSMLRSYRQMSVISLASMHSDCSTPSKVPAESFDLESAPPKTPKVEEE PISPGSTLPEVKLRRSKKRTKRSSVVFADEKAATESDLKRLSRKQEFMSDTNLSEHAAIPARVSILSQMS FASQSMPTIPALTLSVAGVPGLDEANTSPRLSQTFFQVSDGDKKTLKKKKVNQFFKTMLASKSSEESKQI PDFLSTNM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_033374
ORF Size	5484 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_033374.3, NP_203538.2
RefSeq Size	6291 bp
RefSeq ORF	5487 bp
Locus ID	94176
UniProt ID	Q8C3J5
MW	212.2 kDa
Gene Summary	Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.[UniProtKB/Swiss-Prot Function]

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