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Grik1 (NM_010348) Mouse Tagged ORF Clone
CAT＃: MR222519
- TrueORF®
Grik1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2
ORF Plasmid: tGFP
"NM_010348" in other vectors (4)
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CNY 6,632.00
CNY 7,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A830007B11Rik; D16Ium2; D16Ium24; D16Ium24e; Glu; GluK; GluK1; GluK5; Glur; Glur-5; Glur5; Glurbe; Glurbeta1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222519 representing NM_010348
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCGGCACAGTCCTTATCCAACCCGGGCTCTGGACCAGGGACACCAGCTGGACACTCCTCTATT TCCTGTGCTACATCCTTCCTCAGACCTCCCCTCAAGTGCTCAGGATCGGAGGGATTTTTGAAACTGTGGA AAACGAACCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGTATTAACCGAAACCGAACC TTGATGCCCAATACCACATTAACCTATGACATCCAGAGAATTAATCTTTTTGATAGTTTTGAAGCCTCCC GAAGAGCATGCGACCAACTGGCTCTTGGGGTGGCCGCCCTCTTCGGTCCTTCCCACAGCTCCTCCGTCAG TGCTGTACAGTCTATTTGCAATGCTCTGGAAGTTCCACACATTCAGACTCGCTGGAAACACCCTTCTGTG GACAACAGAGACTTATTTTACATCAACCTCTACCCAGATTATGCAGCTATCAGCAGGGCAGTCCTGGATC TGGTCCTCTATTACAACTGGAAAACAGTGACGGTGGTGTACGAAGACAGCACAGGTCTAATTCGTCTGCA AGAGCTCATCAAAGCTCCCTCCAGATACAACATCAAAATCAAAATCCGCCAGCTTCCCTCTGGCAATAAG GATGCCAAACCTCTGCTCAAGGAGATGAAGAAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCGCACG AGACGGCTGCTGAAATTCTTAAGCAGATTTTGTTCATGGGCATGATGACTGAATACTATCACTACTTCTT CACAACCCTGGACTTGTTTGCTTTGGATCTGGAACTCTACAGGTACAGTGGTGTAAATATGACTGGATTT CGGTTGCTGAATATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGATTGCAGG CCCCACCCAGACCTGAGACTGGTCTCCTGGACGGCGTGATGACAACTGAAGCAGCTCTGATGTACGATGC TGTGTACATGGTAGCCATCGCCTCTCACCGTGCCTCTCAGCTGACCGTCAGTTCCCTGCAGTGCCATCGA CATAAGCCATGGCGCCTAGGACCCAGATTTATGAACCTCATCAAAGAGGCGCGGTGGGATGGCTTGACGG GGCGGATCACCTTCAATAAGACGGATGGCTTGAGAAAGGATTTTGACCTGGACATTATCAGTCTCAAAGA GGAAGGAACTGAAAAGATTGGGATTTGGAACTCCAACAGTGGGCTGAACATGACGGATGGCAACAGAGAC AGGTCCAACAATATCACAGATTCGCTGGCTAACCGAACGCTCATTGTCACCACTATTCTGGAAGAGCCCT ACGTGATGTACAGGAAATCCGATAAACCACTGTACGGAAATGACAGATTTGAAGGATATTGCCTGGATCT GCTGAAAGAACTGTCAAATATCCTAGGTTTCCTTTATGATGTTAAACTGGTTCCTGACGGCAAATATGGA GCCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAGGAACTCATCGACCACAGAGCTGACCTAGCAG TGGCCCCTCTCACCATCACGTATGTACGGGAGAAAGTCATTGACTTCTCCAAGCCTTTCATGACTCTGGG CATTAGCATCCTTTACCGGAAGCCCAATGGAACCAACCCCGGCGTCTTCTCCTTCCTCAACCCCCTGTCT CCAGACATTTGGATGTATGTGCTCCTCGCTTGCCTAGGAGTCAGTTGTGTGCTTTTTGTGATTGCAAGGT TCACACCCTACGAGTGGTATAACCCCCACCCGTGCAACCCTGACTCAGACGTGGTGGAAAACAATTTCAC TTTGCTAAATAGTTTCTGGTTTGGCGTTGGAGCTCTCATGCGGCAAGGATCGGAGCTGATGCCCAAGGCT CTATCGACCAGAATAGTTGGAGGAATATGGTGGTTTTTCACCCTAATCATCATCTCATCCTACACTGCCA ACCTGGCTGCCTTCTTGACAGTAGAAAGGATGGAATCCCCCATCGATTCCGCAGACGACCTGGCCAAACA AACCAAGATAGAATACGGGGCAGTCAGAGATGGCTCGACAATGACCTTCTTCAAGAAATCAAAAATCTCC ACGTATGAGAAAATGTGGGCTTTCATGAGCAGTAGACAGCAGAGCGCCCTGGTTAAAAACAGCGATGAGG GGATCCAAAGGGTGCTTACCACCGACTACGCCCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACACA GAGGAACTGCAACCTCACTCAGATCGGGGGCCTCATAGACTCCAAAGGCTATGGAGTGGGGACACCTATC GGCTCCCCTTACCGGGATAAAATTACAATTGCTATTCTTCAACTACAAGAAGAAGGGAAGCTTCATATGA TGAAAGAGAAATGGTGGAGGGGAAATGGCTGCCCTGAAGAAGACAGTAAAGAAGCCAGTGCTCTAGGAGT GGAAAATATCGGGGGTATCTTCATTGTTCTGGCTGCAGGACTCGTCCTTTCTGTGTTTGTAGCCATTGGA GAATTCATATACAAATCACGGAAGAACAATGACATTGAGCAGTGTCTCTCTTTCAATGCCATCATGGAAG AGCTGGGAATCTCACTCAAGAATCAGAAAAAATTAAAGAAAAAGTCAAGAACTAAGGGCAAATCTTCTTT CACAAGTATCCTTACTTGTCATCAGAGACGAACTCAGAGAAAAGAGACTGTGGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222519 representing NM_010348
Red=Cloning site Green=Tags(s) MERGTVLIQPGLWTRDTSWTLLYFLCYILPQTSPQVLRIGGIFETVENEPVNVEELAFKFAVTSINRNRT LMPNTTLTYDIQRINLFDSFEASRRACDQLALGVAALFGPSHSSSVSAVQSICNALEVPHIQTRWKHPSV DNRDLFYINLYPDYAAISRAVLDLVLYYNWKTVTVVYEDSTGLIRLQELIKAPSRYNIKIKIRQLPSGNK DAKPLLKEMKKGKEFYVIFDCSHETAAEILKQILFMGMMTEYYHYFFTTLDLFALDLELYRYSGVNMTGF RLLNIDNPHVSSIIEKWSMERLQAPPRPETGLLDGVMTTEAALMYDAVYMVAIASHRASQLTVSSLQCHR HKPWRLGPRFMNLIKEARWDGLTGRITFNKTDGLRKDFDLDIISLKEEGTEKIGIWNSNSGLNMTDGNRD RSNNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFLYDVKLVPDGKYG AQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLS PDIWMYVLLACLGVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMRQGSELMPKA LSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKIS TYEKMWAFMSSRQQSALVKNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQIGGLIDSKGYGVGTPI GSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDSKEASALGVENIGGIFIVLAAGLVLSVFVAIG EFIYKSRKNNDIEQCLSFNAIMEELGISLKNQKKLKKKSRTKGKSSFTSILTCHQRRTQRKETVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010348 |
| ORF Size | 2715 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010348.3, NP_034478.1 |
| RefSeq Size | 3573 bp |
| RefSeq ORF | 2718 bp |
| Locus ID | 14805 |
| MW | 103.2 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222510 | Grik1 (untagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2, (10ug) |
CNY 11,690.00 |
|
| MG222519 | Grik1 (tGFP-tagged) - Mouse glutamate receptor ionotropic kainate 1 (Grik1) transcript variant 2, (10ug) |
CNY 8,232.00 |
|
| MR222519L3 | Lenti ORF clone of Grik1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2 |
CNY 9,790.00 |
|
| MR222519L4 | Lenti ORF clone of Grik1 (mGFP-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2 |
CNY 9,790.00 |
