Ptprr (NM_001161840) Mouse Tagged ORF Clone

CAT#: MR221795

  • TrueORF®

Ptprr (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, R (Ptprr), transcript variant 5

ORF Plasmid: DDK tGFP



  "NM_001161840" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Gmcp1; mPTP213; PTP-SL; PTPBR7; RPTPRR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR221795 representing NM_001161840
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTATTTACAGGTTAAAAGAAAGGCTTCAGCTTTCCTTAAGACAAGATAAAGAGAAAAACCAGGAGA
TCCACCTATCACCCATTGCACGGCAGCAAGCACAATCGGAGGCCAAGACGACCCACAGCATGGTCCAGCC
CGATCAGGCGCCAAAGGTGCTGAACGTGGTTGTGGACCCTCAAGGCCAATGCACTCCTGAGATTCGAAAC
AGCACCTCCACCTCTGTCTGCCCTTCTCCCTTCAGAATGAAGCCCATAGGACTCCAGGAGCGACGAGGTT
CCAATGTATCTCTTACGCTGGACATGAGTAGCCTGGGCAGTGTGGAACCCTTTGTGGCCGTCTCAACCCC
CCGGGAGAAGGTAGCCATGGAATACCTGCAGTCAGCCAGCCGAGTTCTCACACGGTCACAGCTGAGGGAC
GTCGTGGCAAGTTCCCACCTACTTCAAAGTGAATTCATGGAAATACCAATGAATTTTGTGGATCCCAAAG
AAATTGATATTCCACGTCACGGAACTAAAAATCGTTATAAGACCATTTTGCCAAATCCCCTCAGCAGAGT
GTGCTTAAGACCAAAAAATATAACCGATTCCTTGAGTACTTACATAAATGCTAACTATATTCGGGGCTAC
AGTGGTAAGGAGAAAGCCTTCATTGCCACCCAGGGCCCCATGATCAACACTGTGAATGACTTCTGGCAGA
TGGTGTGGCAAGAAGACAGTCCCGTGATTGTGATGATCACGAAACTCAAAGAGAAAAATGAGAAATGTGT
GCTCTACTGGCCAGAAAAGAGAGGGATTTACGGCAAGGTTGAGGTTCTGGTCACCGGTGTGACCGAATGT
GATAACTACACCATCCGCAACCTCGTCTTAAAGCAAGGAAGTCACACCCAACATGTGAAGCACTACTGGT
ACACTTCATGGCCGGATCATAAGACTCCAGACAGTGCCCAGCCCCTTCTGCAGCTCATGTTGGATGTGGA
AGAAGACAGACTGGCCTCTGAAGGCCGAGGGCCTGTGGTTGTCCACTGCAGTGCGGGGATTGGGAGAACT
GGGTGTTTCATCGCTACATCCATTGGCTGTCAACAATTGAAAGAAGAAGGAGTTGTAGACGCACTAAGTA
TTGTCTGCCAGCTTCGTGTAGACAGGGGTGGTATGGTCCAAACCAGCGAGCAGTATGAATTTGTGCACCA
TGCTCTGTGCCTGTTCGAGAGCAGACTTTCACCAGAAACTGTCGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR221795 representing NM_001161840
Red=Cloning site Green=Tags(s)

MIIYRLKERLQLSLRQDKEKNQEIHLSPIARQQAQSEAKTTHSMVQPDQAPKVLNVVVDPQGQCTPEIRN
STSTSVCPSPFRMKPIGLQERRGSNVSLTLDMSSLGSVEPFVAVSTPREKVAMEYLQSASRVLTRSQLRD
VVASSHLLQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNITDSLSTYINANYIRGY
SGKEKAFIATQGPMINTVNDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVTGVTEC
DNYTIRNLVLKQGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASEGRGPVVVHCSAGIGRT
GCFIATSIGCQQLKEEGVVDALSIVCQLRVDRGGMVQTSEQYEFVHHALCLFESRLSPETVE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001161840
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001161840.1, NP_001155312.1
RefSeq Size 2700 bp
RefSeq ORF 1239 bp
Locus ID 19279
UniProt ID Q62132
MW 47 kDa
Gene Summary Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gamma may have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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