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Tmem67 (NM_177861) Mouse Tagged ORF Clone
CAT＃: MR221073
- TrueORF®
Tmem67 (Myc-DDK-tagged) - Mouse transmembrane protein 67 (Tmem67)
ORF Plasmid: tGFP
"NM_177861" in other vectors (4)
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CNY 10,928.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5330408M12Rik; b2b1163.1Clo; b2b1291.1Clo; B230117O07 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221073 representing NM_177861
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTACCATGGTGACGCGTACAAGGCCTGTGGCGGCCATGGCGGTCAGATCTCGTTCTTCATCCCGGA CTGGCACCGCCTACCTTCTCTTAGTTCTCTGCGAAGTCTCATGGGCACAAATCTTCTCCTTCCCTTTCCG GCGGCCTGAGACCTGCGACTTCAACCAGTACTTTGACATCTCGGCGCTCTCCTGTGCCCCCTGTGGCGCC AACCAGCGGCGGGATGCACTAGGAACTTCCTGTGTATGTCTACCAGGATATCATATGATCTCTAATAATG GAGGACCTTCTATTATTTGTAAAAAGTGTCCAGAAAACATGAAAGGCGTTACTAAAGATGGCTGGGACTG CATTTCTTGTCCTAGTGGCTTAACTGCTGAGGGGAAGTGCCACTGTCCCACTGGCCATATATTAGTGGAA AGAAATGTTAGTGGGTCTTTGTTAGCCCAAGCAACTTGCGAGCTTTGTGATGAAAGTGAAAACTCATTTA CAAAAGCCAATGCTTTAGGAACCAGGTGTGTCCGCTGCGAACCAACATTTGTGAATACAAGCAGGTCCTG TTCATGTTCAGAGCCTCACACTTTAACAGGTGGATTATGTTTCAGCAACACAGGGAATTTTCATCAACGT GTAATTTCCACTGCACGTTATGGAGAGCTTGGCATGTCATTAAATTCAGAATGGTTTGCCAAGTATTTGC AGGCAACAGCAGCTGCATGCTGGACTCACGCCAACCTGACATCGTGCCAAGCTCTTGGAAATATGTGCGT GATGAACATGAACTCTTACGACTCGACCACACTCGATGCTTGTCGACTGTTCCATTACATCTTTGAAAGC ACTGCTGGATTGATCAGTGTGCATTCTGTTCCATTCTGGAGACAGAACCTTCCTTGGCTGTTCTATGGAG ACCAGCCAGGATTGGCTCCTCAAGTTCTCAGTACTACACCTCTTCCTACAAATTTCAGTTTTAAAGGACA AAACCAGCTGAAGTTTGTTGCTGCTTCCTATGATATACGAGGAAACTTCATCAAATGGCAACCTTTAGAA GGTGGTGTTTTACAGCTTTGTCCAGACACTGAGAGAAGGCTGGATGCAGCGTATGCTTTTGGAACAACGT ACCAGCAAAATTGTGAAATCTCTCTCTCTAAACTCCTGGTTGACTTCAGCAGCCCTGTATTTTATGATGT TTACCTCGAATACACTGATGAAGAGCAGCACCGTTACCTTTGGCCTATCCCAGTGTTAAACCTAAATCTT CAACACAACAAACTGTTTGTGAACCAAGACAGTAGCTCCAGCAAGTGGCTTCTGACTCGGCGCATTTTCT TAGTGGATGCAGTAAGTGGGCGAGAAAATGACCTAGGAAATCAGCCAAGAGTAATTCGAGTTGCTACCCA GATTTCACTGAGCATACGCCTTGTACCCAACACAAAAAATGGAAACATATACACTCCCCTACTTACCATT GCCTACAGTGACATTGACATCAAAAATGCTCATAGCCAGTCTGCGAAGATTTCCTTCTCAGTGAAGTATG AGATGAATCAAGGAGACGCATCTGTGCACACAGATATTGCGTTGGGTGTGTTGGGTGGGCTGGCTGTGCT ATCATCTCTTTTGAAGACAGCGGGATGGAAGAGGCGCATCGGGAGCCCCATGATTGATTTACAGACAGTT ATGAAATTCCTGCTGTACTATGCTGGTGATCTGGCCAATGTGTTTTTTATCATCACAGTGGGAACAGGTC TTTATTGGCTTATTTTCTTCAAAGCACAGAAGTCAGTCTCTGTTTTGCTGCCGATGCCAGTTCAGGAAGA ACGTTTCGTTACTTATGTGGGGTGCGCCTTTGCTATGAAGGCTCTCCAGTTTTTACATAAGTTCATATCC CAGATTTCCATAGATATATTCTTTATTGATTGGGAACGGCCGAAAGGAAAAGTTCTTAAAGCTGTTGAAG GTGAGGGTGGTGTGCGGAGTGCCACTGTTCCTGTGAGCATATGGAGAACGTATTTCGTGGCAAATGAATG GAATGAGATTCAGACTGTGAGAAAAATTAATCCACTCTTTCAAGTGCTTACCACACTCTTCTTCTTGGAG GTTGTGGGATTCAAAAATTTAGCGTTAATGGATTCATCCTCCAGCCTTTCTAGAAACCCATCAGACTACA CAGCTCCTTACAGCCGCATTCTGAGATATGCCGTGGCTACTGCTATCTGGCTGGTGATTGGAATAATACA GGTTGTTTTCTTTGCTGCCTTTTATGAGAGATTCATAGAAGACAAAATCCGGCAGTTTGTTGACCTGTGC TCCATGAGTAATGTGTCAGTGTTCCTGCTGTCCCACAGATGTTTTGGATATTACATTCATGGGAGGTCAG TCCACGGCCATGCAGACACTAACATGGAGGAAATGAATATGAACCTTAAGAGAGAGGCGGAAAATTTGTG CAGCCAAAGAGGTTTGGTGCCCAACACGGATGGGCAGACGTTTCAGATTGCAGTTTCTAGTCAGATGAGA CAACACTATGACAGGATTCATGAGACTTTAACAAGGAGAAATGGCCCTGCCAGACTGCTGAGTTCGTCGG GGAGTACTTTTGAACAAAGCATCAAAGCATACCACGCAATGAATAAGTTCCTTGGCTCTTTCATTGACCA CGTTCATAAGGAAATGGATTACTTTATAAAAGATAAGTTGCTTCTTGAAAGAATCCTTGGGATGGAATTC ATGGAGCCCATGGAGAAGAGCATCTTCTATAATGATGAAGGCCATTCTTTCAGCAGTGTGCTGTATTATG GGAACGAGGCCACGCTCCTCATCTTTGATCTGTTGTTCTTCTGTGTTGTGGATTTGGCTTGCCAGGACTT TGTTTTAGCATCCTTCCTCACATACCTGCAACAAGAGATTTTCCGATTTATCCGAAATACAGTAGGACAA AAGAATTTGGCAACCAAAACACTAGTGGATGAAAGATTTCTGATC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR221073 representing NM_177861
Red=Cloning site Green=Tags(s) MGTMVTRTRPVAAMAVRSRSSSRTGTAYLLLVLCEVSWAQIFSFPFRRPETCDFNQYFDISALSCAPCGA NQRRDALGTSCVCLPGYHMISNNGGPSIICKKCPENMKGVTKDGWDCISCPSGLTAEGKCHCPTGHILVE RNVSGSLLAQATCELCDESENSFTKANALGTRCVRCEPTFVNTSRSCSCSEPHTLTGGLCFSNTGNFHQR VISTARYGELGMSLNSEWFAKYLQATAAACWTHANLTSCQALGNMCVMNMNSYDSTTLDACRLFHYIFES TAGLISVHSVPFWRQNLPWLFYGDQPGLAPQVLSTTPLPTNFSFKGQNQLKFVAASYDIRGNFIKWQPLE GGVLQLCPDTERRLDAAYAFGTTYQQNCEISLSKLLVDFSSPVFYDVYLEYTDEEQHRYLWPIPVLNLNL QHNKLFVNQDSSSSKWLLTRRIFLVDAVSGRENDLGNQPRVIRVATQISLSIRLVPNTKNGNIYTPLLTI AYSDIDIKNAHSQSAKISFSVKYEMNQGDASVHTDIALGVLGGLAVLSSLLKTAGWKRRIGSPMIDLQTV MKFLLYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPVQEERFVTYVGCAFAMKALQFLHKFIS QISIDIFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINPLFQVLTTLFFLE VVGFKNLALMDSSSSLSRNPSDYTAPYSRILRYAVATAIWLVIGIIQVVFFAAFYERFIEDKIRQFVDLC SMSNVSVFLLSHRCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFQIAVSSQMR QHYDRIHETLTRRNGPARLLSSSGSTFEQSIKAYHAMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEF MEPMEKSIFYNDEGHSFSSVLYYGNEATLLIFDLLFFCVVDLACQDFVLASFLTYLQQEIFRFIRNTVGQ KNLATKTLVDERFLI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177861 |
| ORF Size | 2985 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177861.4, NP_808529.2 |
| RefSeq Size | 3456 bp |
| RefSeq ORF | 2988 bp |
| Locus ID | 329795 |
| UniProt ID | Q8BR76 |
| MW | 112.6 kDa |
| Gene Summary | Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology. Essential for endoplasmic reticulum-associated degradation (ERAD) of surfactant protein C (sftpc).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223048 | Tmem67 (untagged) - Mouse transmembrane protein 67 (Tmem67), (10ug) |
CNY 10,944.00 |
|
| MG221073 | Tmem67 (tGFP-tagged) - Mouse transmembrane protein 67 (Tmem67), (10ug) |
CNY 10,830.00 |
|
| MR221073L3 | Lenti ORF clone of Tmem67 (Myc-DDK-tagged) - Mouse transmembrane protein 67 (Tmem67) |
CNY 11,780.00 |
|
| MR221073L4 | Lenti ORF clone of Tmem67 (mGFP-tagged) - Mouse transmembrane protein 67 (Tmem67) |
CNY 11,780.00 |
