Myo10 (NM_019472) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW048724; D15Ertd600e; mKIAA0799; myosin-X
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR220971 representing NM_019472 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGCTTCTTTCCCGAGGGAGCACGGGTCTGGCTAAGAGAAAATGGCCAGCATTTTCCGAGTACTG TAAATTCCTGTGCAGAAGGCGTCGTGGTCTTCCAGACAGACTATGGGCAGGTGTTCACCTACAAGCAGAG TACAATCACCAACCAGAAGGTGACAGCTATGCACCCCCTGCACGAGGAGGGCGTGGACGACATGGCTTCC CTGGCCGAGCTCCATGGGGGCTCCATCATGTACAACCTTTTCCAGCGCTACAAAAGGAACCAAATCTATA CCTACATCGGCTCCATCATCGCGTCAGTGAACCCCTACCAGCCCATCGCTGGCCTGTACGAGCGCGCCAC CATGGAGGAGTACAGCAGGTGCCACCTGGGCGAGCTTCCACCGCACATCTTCGCCATTGCCAACGAATGC TATCGCTGCTTGTGGAAGCGCCATGACAACCAATGTGTCCTCATCAGCGGGGAAAGCGGAGCTGGGAAAA CGGAAAGCACCAAGCTGATCCTCAAGTTCCTGTCCGTCATCAGCCAGCAGACTTTGGACCTGGGCCTGCA GGAGAAGACATCCAGCGTCGAGCAAGCCATCCTTCAAAGCAGCCCAATCATGGAAGCATTTGGCAATGCG AAGACAGTATACAACAACAACTCCAGTCGCTTCGGGAAGTTTGTTCAGCTGAACATCTGCCAGCAGGGAA ACATTCAGGGAGGAAGAATTGTAGATTATTTATTAGAAAAAAACCGAGTAGTGAGGCAGAATCCCGGGGA GAGGAATTATCACATATTCTATGCACTGCTGGCAGGGCTGGACCAGGGAGAGCGAGAAGAATTTTATTTA TCTTTGCCAGAAAACTACCATTACTTGAATCAGTCTGGGTGTACGGAAGACAAGACGATCAGCGACCAGG AATCCTTCAGACAAGTTATCACGGCAATGGAAGTCATGCAATTCAGCAAGGAGGAAGTTCGGGAAGTTTT GAGGTTGCTGGCGGGAATTCTGCATCTCGGCAACATAGAGTTTATCACTGCTGGAGGGGCGCAGATTCCC TTCAAAACAGCTTTGGGTAGGTCTGCGGACTTGCTTGGGCTGGACCCAACGCAGCTCACAGATGCACTGA CCCAGAGATCCATGATCCTCAGGGGAGAAGAGATCCTGACGCCTCTCAGTGTCCAGCAGGCCGTAGATAG CAGAGACTCATTGGCCATGGCACTTTATGCTCGCTGCTTTGAGTGGGTGATCAAGAAGATCAACAGCCGG ATCAAAGGCAAAGACGACTTCAAATCCATCGGCATCCTTGACATCTTCGGATTTGAAAACTTCGAGGTTA ACCACTTTGAGCAGTTCAATATCAACTATGCAAATGAAAAGCTTCAGGAATACTTCAACAAACACATTTT TTCCTTAGAACAACTAGAATATAGCAGGGAGGGATTGGTGTGGGAAGATATTGACTGGATAGACAATGGA GAATGTCTAGACCTGATCGAGAAGAAACTTGGCCTCCTCGCCTTGATCAATGAAGAGAGCCATTTTCCTC AAGCCACAGACAGCACTTTGTTGGAGAAGCTACACAGTCAGCATGCTAATAACCACTTTTATGTGAAACC CCGCGTTGCAGTCAACAATTTTGGCGTGAAGCATTATGCAGGAGAGGTGCAGTATGATGTCCGAGGCATC TTGGAAAAGAACAGAGACACATTCAGAGATGACCTTCTCAACCTGCTCAGGGAAAGCAGGTTCGACTTCA TCTATGACCTCTTTGAACACGTCTCCAGCCGCAACAACCAGGACACCTTGAAATGCGGAAGCAAACACCG GCGGCCCACTGTCAGCTCGCAGTTCAAGGACTCCCTGCACTCCTTAATGGCAACACTAAGCTCCTCAAAT CCTTTCTTTGTCAGATGCATCAAGCCAAACACACAGAAGATGCCAGACCAGTTTGATCAGGTGGTAGTGC TCAACCAGCTGCGCTACTCGGGGATGCTGGAGACGGTGAGGATTCGGAAAGCAGGGTATGCCGTCAGAAG ACCCTTTCAGGACTTTTACAAAAGGTATAAAGTGCTGATGAGGAACTTGGCCCTGCCTGACGACATCCGA GGGAAGTGCACTGTGCTCCTGCAGGTCTATGACGCTTCTAACAGCGAGTGGCAGCTGGGGAAGACCAAGG TCTTCCTTCGAGAATCCTTGGAGCAGAAACTGGAAAAGAGGCGGGAAGAGGAGATTGACCGTGCGGCCAT GGTGATCCGAGCTCACATCTTAGGCTACTTGGCACGAAAGCAATATAGAAAGGTCCTCTGTGGTGTGGTG ACCATACAGAAGAATTACAGAGCTTTCCTTGCGAGGAAGAAATTTTTGCACCTGAAAAAGGCAGCCATCG TGTTCCAGAAGCAACTGCGAGGACAGCTGGCACGCAGGGTTTACAGGCAGCTGTTGGCTGAGAAACGGGA GCTGGAGGAAAAGAAGAGGCGGGAGGAAGAGAAGAAGAGAGAGGAAGAGGAAAGAGAAAGAGAGCGAGCG CAAAGAGAAGCTGACCTCCTCCGTGCCCATCAGGAGGCAGAGACAAGGAGGCAGCAAGAACTGGAAGCCT TGCAGAAGAGCCAGAGGGAAGCGGACTTGACCAGGGAGCTGGAAAAGCAAAGGGAGAACAAGCAGGTGGA GGAGATCCTCCGCCTGGAGAAGGAAATCGAGGATCTGCAGCGCATGAAGGAGCGGCAGGAGCTGTCGTTG ACCGAGGCCTCCCTGCAGAAGCTGCAGCAGCTGCGGGATGAGGAGTTGCGCAGGCTGGAGGATGAGGCCT GCCGCGCCGCCCAGGAGTTCTTGGAGTCGCTCAACTTCGACGAGATCGACGAGTGCGTCCGCAACATCGA GCGCTCCCTGTCGGTAGGGAGCGAGATTTCGGGCGAAGAGCTCAGTGAGCTTGCCGAGAGTGCCAGCGGA GAGAAGCCAAACTTCAACTTCAGCCAGCCCTACCCCGCGGAGGAGGAGGTGGACGAGGGCTTCGAGGCGG ATGACGACGCCTTCAAGGACTCGCCCAACCCCAGCGAGCACGGCCACTCCGACCAGCGAACGAGTGGCAT CCGGACGAGCGACGACTCATCCGAGGAGGACCCTTACATGAACTACACGGTGGTGCCCACCAGCCCCAGT GCCGACAGTACGGTGCTTCTTGCTGCGTCCATGCAGGACTCCGCTAGCCTGCACAACTCCTCGAGTGGGG AGTCCACCTACTGCATGCCGCAGAACAACGGCGACCTGCCCTCCCCCGACGGCGACTATGACTATGACCA GGACGATTACGAGGATGGCGCCATCACGTCAGGCAGCAGCGTGACTTTCTCCAACTCCTATGGAAGCCAG TGGTCCCCCGACTACAGGTATTCCGTGGGAACCTACAACAGTTCCGGAGCGTACCGGTTCAGCTCAGAAG GGGCGCAGTCCTCCTTTGAAGACAGCGAGGAGGACTTCGACTCCAGGTTTGATACAGATGATGAACTTTC CTACCGGCGAGACTCTGTGTATAGCTGCGTCACACTGCCCTATTTCCACAGTTTTCTGTACATGAAAGGT GGCTTAATGAACTCCTGGAAGCGCCGCTGGTGTGTCCTGAAGGACGAGACCTTCCTGTGGTTCCGCTCCA AGCAGGAAGCCCTCAAGCAAGGCTGGCTGCACAAGAAAGGCGGGGGCTCCTCCACGCTGTCCCGGAGGAA CTGGAAGAAGCGCTGGTTTGTCCTTCGCCAGTCGAAGCTGATGTACTTTGAAAACGATAGCGAGGAGAAG CTCAAGGGCACCGTGGAAGTCCGCACCGCCAAGGAGATCATAGACAACACAAGCAAAGAGAACGGGATCG ACATCATCCTGGCCGACAGGACCTTCCATCTCATCGCGGAGTCCCCCGAAGATGCCAGCCAATGGTTCAG CGTGCTGAGTCAGGTCCACTCGTCCACAGACCAGGAGATCAGAGAAATGCATGATGAGCAGGCAAACCCC CAGAATGCTGTGGGCACACTGGATGTCGGGCTGATTGATTCTGTATGCGCCTCTGACAGCCCTGATAGAC CCAACTCATTTGTGATCATCACAGCCAACCGGGTGCTGCACTGTAACGCGGACACCCCAGAGGAGATGCA CCACTGGATAACCCTGCTGCAGAGATCCAAGGGGGACACCAGGGTGGAGGGCCAGGAGTTCATCGTCAGA GGGTGGCTGCATAAGGAAGTGAAGAACAGTCCGAAGATGTCCTCCCTGAAACTGAAGAAACGGTGGTTCG TGCTGACGCACAATTCCCTGGACTACTACAAGAGCTCAGAGAAGAACGCTCTGAAACTGGGCACTCTGGT CCTCAACAGCCTGTGCTCCGTTGTGCCCCCAGACGAGAAGATATTCAAAGAGACAGGCTACTGGAATGTC ACCGTGTATGGGCGCAAACACTGTTACCGACTCTACACGAAACTACTGAACGAGGCGACTAGGTGGTCCA GCGCCATCCAGAATGTGACTGACACCAAGGCTCCAATCGACACGCCCACCCAGCAGCTGATCCAAGATAT TAAGGAGAACTGCCTCAACTCTGACGTGGTGGAGCAGATTTACAAGCGGAACCCGATCCTTCGCTACACG CATCACCCGCTGCACTCCCCGCTCCTGCCTCTTCCCTATGGGGACATAAATCTCAACCTGCTGAAAGACA AAGGCTACACGACCCTTCAGGACGAAGCCATCAAGATATTCAATTCTCTCCAACAACTGGAGTCCATGTC CGACCCCATCCCTATCATCCAGGGCATCCTGCAGACCGGGCACGACCTGCGGCCTCTGCGGGATGAACTG TATTGTCAGCTCATCAAACAGACCAACAAGGTGCCCCATCCCGGCAGCGTGGGCAACCTTTACAGCTGGC AGATCCTGACCTGCCTCAGCTGCACCTTCCTGCCGAGCCGCGGGATCCTCAAATACCTCAAGTTCCACCT GAAAAGGATACGGGAACAGTTCCCAGGAACCGAGATGGAGAAGTACGCCCTCTTTATCTACGAATCCCTT AAGAAAACCAAATGCAGAGAGTTTGTGCCTTCCCGAGATGAAATCGAAGCTCTGATCCACCGGCAGGAAA TGACGTCCACAGTGTATTGCCATGGCGGAGGCTCCTGCAAGATCACCATCAACTCCCACACCACAGCCGG GGAGGTGGTGGAGAAGCTGATCCGAGGGCTCGCCATGGAGGACAGCAGGAACATGTTTGCCTTGTTTGAG TACAACGGACAGGTGGACAAGGCCATCGAAAGCCGGACCATCGTAGCTGACGTCCTGGCAAAGTTTGAAA AGCTAGCTGCCACGTCAGAAGCCGGGGACGCGCCATGGAAATTCTACTTCAAACTTTATTGCTTCCTGGA CACCGACAGCATGCCAAAAGACAGCGTGGAGTTTGCGTTTATGTTTGAACAGGCCCACGAAGCCGTCATC CATGGCCACCACCCAGCCCCTGAGGAAAGCCTCCAAGTGCTGGCTGCCCTGAGACTGCAGTATCTGCAGG GCGACTATACCCCGCACACCTCCATCCCACCCCTCGAGGAGGTTTACTCTGTGCAGAGACTCAGAGCTCG CATTAGTCAGTCGACCAAAACCTTCACCCCGTACGAGCGCCTCGAGAAGAGACGGACCAGCTTCCTGGAG GGCACGCTGCGGCGGAGCTTCCGGACCGGGTCGGTGGTTCGGCAGAAGGCGGAGGAGGAGCAGATGCTGG ACATGTGGATCAAGGAGGAAGTCTGCTCGGCTCGCACCAGCATCATTGACAAGTGGAAGAAACTGCAGGG GATGAACCAGGAACAAGCCATGGCCAAGTATATGGCCCTGATCAAGGAGTGGCCTGGTTATGGATCAACA CTCTTTGATGTGGAGTGCAAAGAAGGCGGCTTCCCCCAGGAGCTCTGGCTGGGCGTCAGCGCAGACGCCG TGTCTGTCTACAAGCGTGGAGAGGGCAAACCGTTAGAAGTCTTCCAGTATGAGCACATCCTCTCGTTCGG GGCACCGCTGGCCAACACGTACAAGATCGTAGTTGACGAGCGGGAGCTGCTCTTTGAGACGAGTGAGGTG GTGGATGTGGCCAAGCTCATGAAAGCGTATATCAGCATGATTGTGAAGAAGCGCTATAGCACCACACGCT CTGTGAGCAGCCAGGGCAGCTCCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR220971 representing NM_019472 Red=Cloning site Green=Tags(s) MDSFFPEGARVWLRENGQHFPSTVNSCAEGVVVFQTDYGQVFTYKQSTITNQKVTAMHPLHEEGVDDMAS LAELHGGSIMYNLFQRYKRNQIYTYIGSIIASVNPYQPIAGLYERATMEEYSRCHLGELPPHIFAIANEC YRCLWKRHDNQCVLISGESGAGKTESTKLILKFLSVISQQTLDLGLQEKTSSVEQAILQSSPIMEAFGNA KTVYNNNSSRFGKFVQLNICQQGNIQGGRIVDYLLEKNRVVRQNPGERNYHIFYALLAGLDQGEREEFYL SLPENYHYLNQSGCTEDKTISDQESFRQVITAMEVMQFSKEEVREVLRLLAGILHLGNIEFITAGGAQIP FKTALGRSADLLGLDPTQLTDALTQRSMILRGEEILTPLSVQQAVDSRDSLAMALYARCFEWVIKKINSR IKGKDDFKSIGILDIFGFENFEVNHFEQFNINYANEKLQEYFNKHIFSLEQLEYSREGLVWEDIDWIDNG ECLDLIEKKLGLLALINEESHFPQATDSTLLEKLHSQHANNHFYVKPRVAVNNFGVKHYAGEVQYDVRGI LEKNRDTFRDDLLNLLRESRFDFIYDLFEHVSSRNNQDTLKCGSKHRRPTVSSQFKDSLHSLMATLSSSN PFFVRCIKPNTQKMPDQFDQVVVLNQLRYSGMLETVRIRKAGYAVRRPFQDFYKRYKVLMRNLALPDDIR GKCTVLLQVYDASNSEWQLGKTKVFLRESLEQKLEKRREEEIDRAAMVIRAHILGYLARKQYRKVLCGVV TIQKNYRAFLARKKFLHLKKAAIVFQKQLRGQLARRVYRQLLAEKRELEEKKRREEEKKREEEERERERA QREADLLRAHQEAETRRQQELEALQKSQREADLTRELEKQRENKQVEEILRLEKEIEDLQRMKERQELSL TEASLQKLQQLRDEELRRLEDEACRAAQEFLESLNFDEIDECVRNIERSLSVGSEISGEELSELAESASG EKPNFNFSQPYPAEEEVDEGFEADDDAFKDSPNPSEHGHSDQRTSGIRTSDDSSEEDPYMNYTVVPTSPS ADSTVLLAASMQDSASLHNSSSGESTYCMPQNNGDLPSPDGDYDYDQDDYEDGAITSGSSVTFSNSYGSQ WSPDYRYSVGTYNSSGAYRFSSEGAQSSFEDSEEDFDSRFDTDDELSYRRDSVYSCVTLPYFHSFLYMKG GLMNSWKRRWCVLKDETFLWFRSKQEALKQGWLHKKGGGSSTLSRRNWKKRWFVLRQSKLMYFENDSEEK LKGTVEVRTAKEIIDNTSKENGIDIILADRTFHLIAESPEDASQWFSVLSQVHSSTDQEIREMHDEQANP QNAVGTLDVGLIDSVCASDSPDRPNSFVIITANRVLHCNADTPEEMHHWITLLQRSKGDTRVEGQEFIVR GWLHKEVKNSPKMSSLKLKKRWFVLTHNSLDYYKSSEKNALKLGTLVLNSLCSVVPPDEKIFKETGYWNV TVYGRKHCYRLYTKLLNEATRWSSAIQNVTDTKAPIDTPTQQLIQDIKENCLNSDVVEQIYKRNPILRYT HHPLHSPLLPLPYGDINLNLLKDKGYTTLQDEAIKIFNSLQQLESMSDPIPIIQGILQTGHDLRPLRDEL YCQLIKQTNKVPHPGSVGNLYSWQILTCLSCTFLPSRGILKYLKFHLKRIREQFPGTEMEKYALFIYESL KKTKCREFVPSRDEIEALIHRQEMTSTVYCHGGGSCKITINSHTTAGEVVEKLIRGLAMEDSRNMFALFE YNGQVDKAIESRTIVADVLAKFEKLAATSEAGDAPWKFYFKLYCFLDTDSMPKDSVEFAFMFEQAHEAVI HGHHPAPEESLQVLAALRLQYLQGDYTPHTSIPPLEEVYSVQRLRARISQSTKTFTPYERLEKRRTSFLE GTLRRSFRTGSVVRQKAEEEQMLDMWIKEEVCSARTSIIDKWKKLQGMNQEQAMAKYMALIKEWPGYGST LFDVECKEGGFPQELWLGVSADAVSVYKRGEGKPLEVFQYEHILSFGAPLANTYKIVVDERELLFETSEV VDVAKLMKAYISMIVKKRYSTTRSVSSQGSSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019472
ORF Size	6186 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019472.2, NP_062345.2
RefSeq Size	8098 bp
RefSeq ORF	6189 bp
Locus ID	17909
UniProt ID	F8VQB6
MW	237.3 kDa
Gene Summary	Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments (By similarity). Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. May play a role in neurite outgrowth and axon guidance. In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Plays a role in formation of the podosome belt in osteoclasts.[UniProtKB/Swiss-Prot Function]

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