Fer (NM_008000) Mouse Tagged ORF Clone
CAT#: MR220922
- TrueORF®
Fert2 (Myc-DDK-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (Fert2), transcript variant 2
ORF Plasmid: tGFP
"NM_008000" in other vectors (4)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AV082135; C330004K01Rik; Fert; Fert2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220922 representing NM_008000
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAAGAGTATGGAGTGTCCCCACTGTGAGGGTGTGCTGGAGCCTGAGTCTGACCCCCAGTTCTCTA AAAAATGTTCTATACCATTGAGCCCTGGACCTTCCTCATCAGAGATCCTGAGATACAAGGAAAGAAAGGA GAGGCTATCCAAATTTGAGTCTATTCGTCATTCAATTGCTGGGATAATTAAGTCTCCAAAGTCGGTACTC GGATCTTCAACAGTGTGTGATGTGATCTCTGTGGGTGAGAGGCCCCTGGCGGAGCATGACTGGTACCATG GTGCCATCCCCAGGATAGAGGCACAGGAACTGCTGAAGCAGCAAGGAGACTTCCTGGTGCGGGAGAGCCA TGGGAAACCTGGTGAATATGTCCTTTCTGTATATTCTGACGGACAAAGGAGGCACTTTATCATACAATTT GTCGATAATCTGTATCGATTCGAGGGCACCGGGTTTTCAAACATTCCCCAGCTTATAGATCACCACTTCA ATACAAAGCAAGTCATCACCAAGAAGTCTGGGGTGGTTCTGCTCAACCCCATCCCAAAGGATAAGAAATG GGTTCTCAATCATGAAGATGTTTCATTGGGAGAATTACTGGGCAAGGGGAATTTTGGTGAAGTGTATAAG GGCACACTAAAGGATAAAACTCCTGTTGCCATTAAAACGTGCAAGGAAGACCTTCCTCAGGAATTAAAAA TAAAGTTTCTACAGGAAGCCAAAATTCTGAAGCAATATGATCATCCCAATATTGTCAAACTGATAGGCGT GTGCACACAAAGACAGCCTGTCTACATCATTATGGAACTGGTCCCAGGGGGTGATTTTCTGACCTTCCTG AGGAAGAGGAAGGACGAGCTGAAGCTGAAGCAGTTGGTGAGATTTTCCTTGGACGTTGCTGCTGGCATGT TGTATCTCGAGAGCAAGAACTGCATTCACAGGGACCTGGCTGCACGGAACTGCCTGGTGGGTGAAAATAA TACTCTGAAAATCAGTGACTTTGGAATGTCTCGGCAAGAAGACGGTGGAGTGTATTCATCTTCTGGCTTA AAGCAGATTCCTATTAAATGGACAGCACCGGAAGCTCTTAATTATGGGAGATACAGTTCTGAAAGTGACG TGTGGAGCTTCGGCATCCTCCTCTGGGAGACCTTCAGCCTAGGAGTCTGCCCGTACCCTGGAATGACAAA CCAGCAAGCGCGAGAGCAAGTGGAGAGAGGATACCGGATGTCAGCCCCACAGAACTGTCCAGAGGAGGTT TTTACAATCATGATGAAGTGTTGGGATTACAAGCCTGAAAACCGCCCTAAGTTCAACGACCTTCACAAAG AGCTCACTGTCATCAAGAAGATGATCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220922 representing NM_008000
Red=Cloning site Green=Tags(s) MDKSMECPHCEGVLEPESDPQFSKKCSIPLSPGPSSSEILRYKERKERLSKFESIRHSIAGIIKSPKSVL GSSTVCDVISVGERPLAEHDWYHGAIPRIEAQELLKQQGDFLVRESHGKPGEYVLSVYSDGQRRHFIIQF VDNLYRFEGTGFSNIPQLIDHHFNTKQVITKKSGVVLLNPIPKDKKWVLNHEDVSLGELLGKGNFGEVYK GTLKDKTPVAIKTCKEDLPQELKIKFLQEAKILKQYDHPNIVKLIGVCTQRQPVYIIMELVPGGDFLTFL RKRKDELKLKQLVRFSLDVAAGMLYLESKNCIHRDLAARNCLVGENNTLKISDFGMSRQEDGGVYSSSGL KQIPIKWTAPEALNYGRYSSESDVWSFGILLWETFSLGVCPYPGMTNQQAREQVERGYRMSAPQNCPEEV FTIMMKCWDYKPENRPKFNDLHKELTVIKKMIT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008000 |
ORF Size | 1359 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008000.2, NP_032026.2 |
RefSeq Size | 2383 bp |
RefSeq ORF | 1362 bp |
Locus ID | 14158 |
UniProt ID | P70451 |
MW | 52.1 kDa |
Gene Summary | Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3 according to PubMed:10878010 and PubMed:19159681, but clearly plays a redundant role in STAT3 phosphorylation. According to PubMed:11134346, cells where wild type FER has been replaced by a kinase-dead mutant show no reduction in STAT3 phosphorylation. Phosphorylates TMF1. Isoform 3 lacks kinase activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215932 | Fert2 (untagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (Fert2), transcript variant 2, (10ug) |
CNY 5,800.00 |
|
MG220922 | Fert2 (tGFP-tagged) - Mouse fer (fms/fps related) protein kinase testis specific 2 (Fert2) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
MR220922L3 | Lenti ORF clone of Fert2 (Myc-DDK-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (Fert2), transcript variant 2 |
CNY 6,460.00 |
|
MR220922L4 | Lenti ORF clone of Fert2 (mGFP-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (Fert2), transcript variant 2 |
CNY 6,460.00 |