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Klhdc8b (NM_030075) Mouse Tagged ORF Clone
CAT＃: MR215866
- TrueORF®
Klhdc8b (Myc-DDK-tagged) - Mouse kelch domain containing 8B (Klhdc8b)
ORF Plasmid: tGFP
"NM_030075" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4931406O17Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215866 representing NM_030075
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGGAGGTGGTCGGGCCTTTGCTTGGCAGGTGTTCCCCCCAATGCCGACTTGCCGGGTATATG GTACAGTGGCACACCAGGACGGACACCTGCTGGTGTTGGGGGGCTGTGGCCGGGCTGGGTTACCTCTGGA TACTGCTGAGACTCTGGACATGGGCTCACACACATGGCTAGCACTAGCACCCCTGCCCACTGCCCGGGCT GGTGCCGCTGCTGTGGTTCTGGGTAAGCAGGTGCTAGTGGTAGGCGGCGTGGACGAAGTCCAAAGCCCAG TAGCTGCTGTGGAGGCCTTCTTGGCTGACGAAGGCCGCTGGGAGCGCCGGGCTACCCTCCCTCAAGCGGC CATGGGTGTTGCGACTGTGGAGAGAGATGGTATGGTGTATGCTCTGGGGGGAATGGGTCCTGACACGGCC CCCCAGGCCCAGGTACTGGTATATGAGCCCCGCCGGGACTGCTGGCTTTCGCTACCCTCCATGCCCACAC CCTGCTATGGGGCCTCCACCTTCCTGCACGGGAACAAGATCTATGTCTTGGGAGGCCGCCAGGGCAAGCT CCCAGTGACTGCTTTTGAAGCTTTTGATCTGGAGACCCGTACATGGACCCGACACCCAAGCCTGCCCAGT CGCAGAGCTTTTGCTGGCTGTGCTATGGCCGAAGGCAGTGTCTTTAGCCTGGGTGGCCTGCAGCAGCCTG GGCCCCACAATTTCTACTCCCGGCCGCACTTTGTCAACACTGTGGAGATGTTTGACCTGGAGCATGGCTC CTGGACTAAGCTGCCTCGTAGCCTGCGGATGAGGGATAAGAGGGCTGACTTTGTGGTTGGCTCCCTTGGG GGCAACATTGTGGCTATTGGGGGCCTTGGGAACCAGCCATGCCCTTTGGCCTCCGTGGAGAGCTTCAGTC TTGCACGGAGACGCTGGGAGGTGCTGCCTGCCATGCCTACGGCCCGATGCTCCTGCTCCAGCCTCCAGGC TGGGCCACGACTGTTTGTTATTGGGGGTGTGGCTCAGGGCCCAAGTCAAGCGGTGGAAGCACTGTGTCTA CGGGATGGAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215866 representing NM_030075
Red=Cloning site Green=Tags(s) MAAGGGRAFAWQVFPPMPTCRVYGTVAHQDGHLLVLGGCGRAGLPLDTAETLDMGSHTWLALAPLPTARA GAAAVVLGKQVLVVGGVDEVQSPVAAVEAFLADEGRWERRATLPQAAMGVATVERDGMVYALGGMGPDTA PQAQVLVYEPRRDCWLSLPSMPTPCYGASTFLHGNKIYVLGGRQGKLPVTAFEAFDLETRTWTRHPSLPS RRAFAGCAMAEGSVFSLGGLQQPGPHNFYSRPHFVNTVEMFDLEHGSWTKLPRSLRMRDKRADFVVGSLG GNIVAIGGLGNQPCPLASVESFSLARRRWEVLPAMPTARCSCSSLQAGPRLFVIGGVAQGPSQAVEALCL RDGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030075 |
| ORF Size | 1062 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030075.2, NP_084351.1 |
| RefSeq Size | 2953 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 78267 |
| UniProt ID | Q9D2D9 |
| MW | 37.6 kDa |
| Gene Summary | Involved in pinching off the separated nuclei at the cleavage furrow and in cytokinesis. Required for mitotic integrity and maintenance of chromosomal stability. Protects cells against mitotic errors, centrosomal amplification, micronucleus formation and aneuploidy. Plays a key role of midbody function involving abscission of the daughter cells during cytokinesis and appropriate chromosomal and nuclear segregation into the daughter cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211866 | Klhdc8b (untagged) - Mouse kelch domain containing 8B (Klhdc8b), (10ug) |
CNY 3,990.00 |
|
| MG215866 | Klhdc8b (tGFP-tagged) - Mouse kelch domain containing 8B (Klhdc8b), (10ug) |
CNY 3,710.00 |
|
| MR215866L3 | Lenti ORF clone of Klhdc8b (Myc-DDK-tagged) - Mouse kelch domain containing 8B (Klhdc8b) |
CNY 5,230.00 |
|
| MR215866L4 | Lenti ORF clone of Klhdc8b (mGFP-tagged) - Mouse kelch domain containing 8B (Klhdc8b) |
CNY 5,230.00 |
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