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Wdfy1 (NM_027057) Mouse Tagged ORF Clone
CAT＃: MR214819
- TrueORF®
Wdfy1 (Myc-DDK-tagged) - Mouse WD repeat and FYVE domain containing 1 (Wdfy1), transcript variant 2
ORF Plasmid: tGFP
"NM_027057" in other vectors (4)
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CNY 1,900.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700013B03Rik; 1700120F24Rik; FENS-1; Jr1; mKIAA1435; WDF1; ZFYVE17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR214819 representing NM_027057
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGATCCACTCCAGGCCTCAGAGCAGCCGCCCGGTGCTGCTGAGCAAGATAGAGGGCCACC AGGACGCCGTCACCGCCGCGCTGCTCATCCCCAAGGAGGACGGCGTGATCACCGCTAGCGAGGACAGAAC CATCCGAGTATGGCTGAAAAGAGACAGCGGCCAGTACTGGCCCAGCATCTACCACACAATGGCCTCCCCT TGCTCTGCCATGGCATACCACCACGACAGCAGGCGGATATTTGTGGGCCAGGATAATGGGGCTGTGATGG AATTTCACGTTTCTGAAGATTTTAATAAAATGAATTTTATCAAGACCTATCCAGCCCACCAGAACCGCGT GTCTGCTATCATCTTCAGCCTGGCTGCCGAGTGGGTGATCAGCACTGGCCACGACAAGTGTGTGAGCTGG ATGTGCACGCGCAGCGGGAATATGCTTGGCCGCCACTTCTTCTCGTCGTGGGCTTCCTGTTTGCAGTATC CTTCAGAGTGCCGGACCCCTCCTCCCATTGTTAGCTGCTTTGAAGACAGAATGACTCCTGTGGATTTATC C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR214819 representing NM_027057
Red=Cloning site Green=Tags(s) MAAEIHSRPQSSRPVLLSKIEGHQDAVTAALLIPKEDGVITASEDRTIRVWLKRDSGQYWPSIYHTMASP CSAMAYHHDSRRIFVGQDNGAVMEFHVSEDFNKMNFIKTYPAHQNRVSAIIFSLAAEWVISTGHDKCVSW MCTRSGNMLGRHFFSSWASCLQYPSECRTPPPIVSCFEDRMTPVDLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027057 |
| ORF Size | 561 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027057.3, NP_081333.1 |
| RefSeq Size | 719 bp |
| RefSeq ORF | 564 bp |
| Locus ID | 69368 |
| MW | 21.6 kDa |
| Gene Summary | Positively regulates TLR3- and TLR4-mediated signaling pathways by bridging the interaction between TLR3 or TLR4 and TICAM1. Promotes TLR3/4 ligand-induced activation of transcription factors IRF3 and NF-kappa-B, as well as the production of IFN-beta and inflammatory cytokines (PubMed:25736436).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210914 | Wdfy1 (untagged) - Mouse WD repeat and FYVE domain containing 1 (Wdfy1), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG214819 | Wdfy1 (tGFP-tagged) - Mouse WD repeat and FYVE domain containing 1 (Wdfy1) transcript variant 2, (10ug) |
CNY 2,090.00 |
|
| MR214819L3 | Lenti ORF clone of Wdfy1 (Myc-DDK-tagged) - Mouse WD repeat and FYVE domain containing 1 (Wdfy1), transcript variant 2 |
CNY 3,800.00 |
|
| MR214819L4 | Lenti ORF clone of Wdfy1 (mGFP-tagged) - Mouse WD repeat and FYVE domain containing 1 (Wdfy1), transcript variant 2 |
CNY 3,800.00 |
