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Gprasp1 (NM_001004359) Mouse Tagged ORF Clone
CAT＃: MR211939
- TrueORF®
Gprasp1 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (Gprasp1), transcript variant 2
ORF Plasmid: tGFP
"NM_001004359" in other vectors (4)
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CNY 13,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2210415K24Rik; 3110031O14Rik; C87852; GASP; GASP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211939 representing NM_001004359
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTAGGGCAGAGGTTGAGCCTGGTGCCCAGGCAAAGGCTGAAAATAAGCCTGGGGATGAGAATGCTA ATGCTGCTGAAGTAGAGCCTGAAGCCCCATTGGTGGTCAGACCCAAGGTTAGGACACAAATCATGACTGG AGCAAGGCCCAAAGTGAAGCCTAAGGGTACCCCTGGAGCAAGACCTAAAGGTGAAACCAGTACACCAGGT GGGGCATATGCCAAATGTAAACCTAAGGCAATCCCTATTGCTCGATCCAAGCATGATGCCCAAGTGTGGG CCCCAAATAAGTTTCGTGGCGAGTCCATGTCAAAGATGGGAAAGCAATGTCAGATCAGTGCTGCAGACCC TCCACTGCTCAGTAATGATTCTGGGATGGTTGCTCAAGCTAAGTGCCTGCCTGTAGATAGAGAACTTGCT AATATGGACACTGAGAGTATTCCCAAAAAGGCTAATTCTCCAGCTGGATTCCAGCCTTCCTATGGCTCAG AGGAGGGAACCAATATGGGGTCCTGGTACCGTGCCAGGCCTGTCCCCAAAGGAGAGGCCTATGAGAATTC CGATTTCAAATGGGCAGATAAACCCTCAGGAAGTCCCTCCTTTTGGAATAGAGATGAAGCCAGTACAAGA TTTCGTCCTAGGAAAAGCATGAAGGCCAATAACAGGTTCAGGCATATGGCCAAACAAGAGGCAAATACCA TGCCTAGGCACAAAAACAAACAAGAGTTCTATAATATTTCCAGTTCTGATTCTGAGGATGAGTCTGGTAA AACTCCCTGGTTCTGGCCCAAAGACAAGACCAAAGTCTGGTCTAAGCCCAAAGAAGAGCCCAATAGTAGG TCCTGGTTTAGGTCTAAGAAAGAAGTCCGTGTTGAATCCACTTCTGGGTCTGAATGTGAAAATCCTACAA AATCTTTGTTTTGGTCTGGAGAAGAGGCTAAATCTAGATCCAAACCCAGAGCCAGGAAAGGGGTCAATAT GAGGGCCAGGCAGCAGGCCAAGCGAGAAGCTTGCAGTGATGCCATGTCTGGAGCTATAGATACAAATAAG AAAGAGTCCTGGTTCTTGCCTGAAGAGAAAGCTAATGTTTTTTCAAAGTCTAAGACAAAGAAAGAGCCCA GAACCAGAGCAGTGCCAAAGGAAGAAGTCAAAACTAAGGCCAGAGCAAGTACCAAGCAAGAAGCCAGGCC AGAGGAGGAAGTCCTCGTTGGGGCTTGGTTCTGGGACACCCAGGAGTCCACTATGGCAGATAGGATAAGC ATCAAGACCACTTTTGTAGAGGAGGAGCCCATTGTTGGAGATTGGTTCTGGAGTGAAGAAGAAGCCAGTG TGGACTCTGAGACCTGTCATACATCCAGACCCAGGGCTAAGGAGGAGCAAGTTAGTAGTTTCTGTTTAGG GTCTGGAAAGAAGAGCAGTATGGAAAGTGGGCCAAAGGCTACTTCTAAATCTATGCCAGTAGCTAAGGAG GATGAGGTCGTTATTGGGTCCTGGTTCTGGGCAGATGATGAAGAGATCAACTTACAGGCTGATGACGAGT CTATTTTTGGATCTTGGTTCTGGGGCACTGGTGAGAACAGCCTTAGATCTGTTGGAGTCAATTGTGAGAA GATGCCGAAGGCTGGAGAAAAAGAAGTTACTGATTCCTGGTTCTGGGCTGGAGATGTCAATACAGAGGCT GAGGTTGAAGAACAGGCCAGGTCGGCATCTACAAAAGCAACAATCTTTGTGCCTTGGTTTTGGTCTGAAA AGCAGCCGAACATGGATTTAGGGTCTGAACCTTGCTCAGATATCATGGCAGGAGCTGAGGAGGAGCCCAT AATTGGGCCCTGGTTCTGGGCTAAAGTAGATAATAGTGTGGAGGCTGAAGTTAACAGTAAGTCTAGCCTT GAGGATGAGGAAGAACCCATTAGATCCCCTTGGTTTGGGGCCAGAGAACAAACCGATATGAAGTATGCAG CTGGTATCAGATACAAGCCTATGGCAGAAGCTGAGGATGCTAACAAAAAGTCTTGTGTCTGGGCAAAAGA ACCCTGTTTGTATCCTACCAATAGAGAATGCTTGAAATCTACTCTGGGAGAAAAAGAAGACACTGTTGAT CCATGGCTCTGGTCCAATAATTATCCAAGGACCAAGACCATCACAGGGTCCTGGCTATGGGCTGCAGAAG AGGGAAATATAGATGATGAGACTGGAGAAAAGATTAAACTACCAACTTTAGAGGACAATGCATTCAATTC TTGGTTCTGGAAAGAAAATGAAGAAAGCATTGTAGAGGCTCCCAAGAGAGAAGAATTCAGGCCAGAAGCG GAAGAGGAAGACATTATTGGTTCTTGGTTTTGGGCTGGGGATGAAGACAGGTTTGAGCCAGCTGCTAAGA TTAATGAAGAGAACAAGATAGCATCTGAAGATGAAGATACAGTTGGATCCTGGTTCTGGGGCAATGAAGA GGCCAGTCTAGAGGCAGTGAGAAGGGGTACTTTTGAGTCTGCTCCTGGGATTAAGGAAGAGAAAGTTACT GGGTCATGGTTCTGGACTGATAAGGCCAAAGTAGGGGCTGGGTCCCAGACTGTCGAAACTGGGTCAGAAA CTGAAGAGGAAGCAATTTTTGAGTCCTTGATCTGGGCTGCAAAAAAGGACAGTATTCAAGCAGGAGTAAA GCGTGTGTCCAAGCCAAAAGATGACGGTAACATAGCTGTTGGGTCCTGGCTCTGGTCTAGTGACAAGGCC ACAAAAGAAGCTAAAACTCTGATTGTCAGTGAGGCCAGTCCAGAAAATGGGAAAGAGTCAGTTGTTAAGT TTGGGTCAAGAGCAAAAGATGAGGTGATTAACAAGACTGGCAGTGGTGACAATTGTAAACATAGTACAGA AGCTGAAACCATAGTGGGAGCCTGGTTCTGGGAAGGAGATGAAGCTAGCTTTGAATCAAATCCTGTACCT GTATGCAAGGCCGTTTGTGAACCCGAGTCTTCAGCTGAGCACGAACCTGACCCTTCCCGCAGGCCTCAGA GCTGGGATGAGGTCACTGTTCAGTTTAAGGCTGGTCCGTGGGGAAAAGCTGGCTTCCCACCCATGAACCC CTTCAGATTCCCCAAAGAAGCGGCATCTCTGTTTGCAGAAATGTTTGGGGGAAAGCCTAAGCTTGTGGAA GTTGGTCCAGAAAGGGAGCCTGAACCCCAGTTCCCATTTCAGTATGATCCCTCTTACCGCTCAGTCCGAG AAATTCGCGAGCATCTTAAGGCCAGGGAAAGTGCACAGCCAGAGAATTGGTCTTGCAACTGCATCCAGTG TGAGCTTAGAATTGGTTCTGAGGAGTTTGAGGAGCTTCTTTTACTGATGGACAGAAATCGTGATCCTTTT ATCCATGAGATATCTAAGATTGCAATGGGCATGCGAGGTGCTTCTCAGTTTACCCGGGATTTCATTCGAA ACTCGGGCGTTGTCTCCCTGATTGAAGCCTTACTTAACTATCCGTCCTCCCGAGTGAGGACAAGGTTTTT GGAAAATATGGTCCGCATGGCTCCCCCTTATCCAGACCTGAACATGATTGAGACATACGTGTGTCAGATT TGCGAGGACACCTTTGACTATGACTTGGATTCCCCTGATCAGTTGTCCGGATTGACAATGATTACACACC TCACTGCTACTTCTGACTATCACAAGGTGGTTGTCAATTACTTGGCTGGGTTTTTCTACTTACTGAATTC AGGCAATACCAAAACAAGGTTTCATGTTCTGAAACTGCTACTGAATTTGTCTGAAAACCTTGTCATGACA AAACGCCTACTTGTTACTGATTCGGTGTCAGAATTTATGGACCTTATTAATAGGGAGGAGTCAGATGAAA ACATTCAAATTGTTCTTGCAATATTTGAGACTATCAGTAAACATATTCAAAAAGAGGCACTGTTTTCTGA TGATGATGATGATGATGAAGAAGAAGATGCAGTGAATCTTGAACCATTTATTTCTGCATTCCGTGAGGCT GAAAAAATTGCCAAGGAACTAAAACGCAAACCTGGCAATCAAAAAGCACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211939 representing NM_001004359
Red=Cloning site Green=Tags(s) MTRAEVEPGAQAKAENKPGDENANAAEVEPEAPLVVRPKVRTQIMTGARPKVKPKGTPGARPKGETSTPG GAYAKCKPKAIPIARSKHDAQVWAPNKFRGESMSKMGKQCQISAADPPLLSNDSGMVAQAKCLPVDRELA NMDTESIPKKANSPAGFQPSYGSEEGTNMGSWYRARPVPKGEAYENSDFKWADKPSGSPSFWNRDEASTR FRPRKSMKANNRFRHMAKQEANTMPRHKNKQEFYNISSSDSEDESGKTPWFWPKDKTKVWSKPKEEPNSR SWFRSKKEVRVESTSGSECENPTKSLFWSGEEAKSRSKPRARKGVNMRARQQAKREACSDAMSGAIDTNK KESWFLPEEKANVFSKSKTKKEPRTRAVPKEEVKTKARASTKQEARPEEEVLVGAWFWDTQESTMADRIS IKTTFVEEEPIVGDWFWSEEEASVDSETCHTSRPRAKEEQVSSFCLGSGKKSSMESGPKATSKSMPVAKE DEVVIGSWFWADDEEINLQADDESIFGSWFWGTGENSLRSVGVNCEKMPKAGEKEVTDSWFWAGDVNTEA EVEEQARSASTKATIFVPWFWSEKQPNMDLGSEPCSDIMAGAEEEPIIGPWFWAKVDNSVEAEVNSKSSL EDEEEPIRSPWFGAREQTDMKYAAGIRYKPMAEAEDANKKSCVWAKEPCLYPTNRECLKSTLGEKEDTVD PWLWSNNYPRTKTITGSWLWAAEEGNIDDETGEKIKLPTLEDNAFNSWFWKENEESIVEAPKREEFRPEA EEEDIIGSWFWAGDEDRFEPAAKINEENKIASEDEDTVGSWFWGNEEASLEAVRRGTFESAPGIKEEKVT GSWFWTDKAKVGAGSQTVETGSETEEEAIFESLIWAAKKDSIQAGVKRVSKPKDDGNIAVGSWLWSSDKA TKEAKTLIVSEASPENGKESVVKFGSRAKDEVINKTGSGDNCKHSTEAETIVGAWFWEGDEASFESNPVP VCKAVCEPESSAEHEPDPSRRPQSWDEVTVQFKAGPWGKAGFPPMNPFRFPKEAASLFAEMFGGKPKLVE VGPEREPEPQFPFQYDPSYRSVREIREHLKARESAQPENWSCNCIQCELRIGSEEFEELLLLMDRNRDPF IHEISKIAMGMRGASQFTRDFIRNSGVVSLIEALLNYPSSRVRTRFLENMVRMAPPYPDLNMIETYVCQI CEDTFDYDLDSPDQLSGLTMITHLTATSDYHKVVVNYLAGFFYLLNSGNTKTRFHVLKLLLNLSENLVMT KRLLVTDSVSEFMDLINREESDENIQIVLAIFETISKHIQKEALFSDDDDDDEEEDAVNLEPFISAFREA EKIAKELKRKPGNQKAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001004359 |
| ORF Size | 4041 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001004359.2, NP_001004359.1 |
| RefSeq Size | 5811 bp |
| RefSeq ORF | 4044 bp |
| Locus ID | 67298 |
| UniProt ID | Q5U4C1 |
| MW | 152.2 kDa |
| Gene Summary | Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224295 | Gprasp1 (untagged) - Mouse G protein-coupled receptor associated sorting protein 1 (Gprasp1), transcript variant 2, (10ug) |
CNY 17,290.00 |
|
| MG211939 | Gprasp1 (tGFP-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (Gprasp1), transcript variant 2 |
CNY 14,730.00 |
|
| MR211939L3 | Lenti ORF clone of Gprasp1 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (Gprasp1), transcript variant 2 |
CNY 15,300.00 |
|
| MR211939L4 | Lenti ORF clone of Gprasp1 (mGFP-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (Gprasp1), transcript variant 2 |
CNY 15,300.00 |
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