Synrg (NM_194341) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ap1gbp1; SYNG
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR211695 representing NM_194341 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCGGCCGGGAGCCGGAGCCAGCGGCGCGGCGGGGGCGGGCGCGGGGCCCGGCGGGGCAGGCA GCTTCATGTTTCCTGTTGCAGGCGGAATGAGACCCCCTCAAGGCCTGATTCCAATGCAACAGCAAGGATT CCCTATGGTCTCTGTCATGCAGCCCAATATGCAAGGCATGATGGGAATGAATTACAGCTCTCAGATGTCC CAAGGACCGATTGCAATGCAGGCAGGAATACCAATGGGGCCAATGCCAGCAGCGGGTGTGCCTTTCCTGG GTCAGCCACCTTTCTTGAGCATGCGTCCTGCAGGCCCACAGTACACTCCAGACATGCAGAAGCAGTTTGC GGAAGAGCAACAAAAACGATTTGAACAACAACAAAAGCTCTTAGAAGAAGAAAGAAAAAGACGTCAGTTT GAAGAGCAAAAGCAAAAGCTCAGACTTCTCAGCAGCGTGAAGCCCAAGACAGGAGAGAAGAACAGAGACG ATGCTTTGGAAGCCATAAAAGGAAACTTGGATGGGTTTTCCAGAGATGCTAAGATGCACCCCACTCCAGC ATCACATCCAAAGAAACAAGGTGTGGGCGTGTTTCCCTCTCAGGACCCTGCTCAGTCCAGAATGCCTCCT TGGATTTACAATGAGAGTTTGGTTCCAGATGCCTATAAGAAAATTTTAGAAACCACAATGACTCCAACTG GAATAGATACTGCTAAACTTTATCCCATTCTGATGTCATCTGGACTTCCCAGGGAAACTCTCGGACAGAT ATGGGCCTTAGCTAATCGAACCACACCTGGCAGGCTCACTAAGGAGGAGCTTTACACGGTTCTCGCCATG GTAGCAGTAACACAGAGGGGTGTTCCTGCCATGAGCCCTGATGCTTTGAGCCAGTTCCCTGCGGCTCCCA TCCCTACCTTAAGCGGCTTTCCTATGACTCTGCCTACCCCAGTGAGCCAGCCAACGGCGATGCCCTCTGG CCCTACAGGCTCCATGCCCCTCACCCTTGGACAACCCATCATGGGTATCAACCTTGTTGGACCAGTGGGT GGAGCTGCAGCACCAACTTCCAGTGGCTTCATGCCAGCCTACCCATCAAACCAGGTGGGAAAGACAGAAG AAGATGACTTCCAGGATTTTCAAGATGCTTCGAAATCAGGATCCATCGATGATTCCTTCACTGACTTCCA AGAGATGCCTGCTTCCTCAAAAACCAGTAATTCCCAGCATGGAAACAGTGCTCCATCACTCTTGATCCCA TTTCCTGGAACTAAAGCCTCCACAGACAAATACGCAGTGTTCAAAGGGATTTCCACTGACAAGCCCTCTG AAAACCCTGCTTCGTTTGGAGAGTCTGGTGATAAATACAGTGCTTTCAGGGAACTCGAGCAAACGACAGA CAGTAAACCTTTAGGAGAGAGCTTTGCCGAGTTCAGATCCACAGGAACCGATGATGGCTTCACTGACTTC AAGACCGCCGATAGTGTGTCACCCCTAGAGCCACCCACAAAAGACACTTTCCCATCAGCTTTTGCCTCAG GAGCTGCACAGCAGACACAGACACAGGTGAAAACCCCTCTGAACTTAGAAGATCTAGATATGTTCTCCTC AGTGGATTGCAGTGGCGAGAAACAGGTGCCCTTTTCAGCTACATTTAGCACAGCAAAGTCCGTTTCCACT CGACCTCAGCCAGCGGGCTCTGCAGCAGCGTCAGCAGCACTGGCATCCACTAAAACTTCTAGTTTGGCAG ATGATTTTGGAGAGTTCAACCTCTTTGGGGAGTATTCAAACCCTGCATCTGCTGGGGAGCAGGACGACTT TGCAGATTTTATGGCTTTCGGTAACAGCTCCATTTCATCTGAGCCAAAAGCAAGTGACAAGTATGAAGCC CTCAGGGAGGAAGTGAGTCCCAGCCCCTTGTCCAGCAGCACTGTGGAAGGGGCACAGCACCCACCTGCTG CGGCAACCAAGTATGATGTCTTCAAACAGCTGTCCCTAGAGGGCGCTGGGCTGGCCATGGAGGAGTTCAA AGAGAACACTTCCTCCACCAAGAGTGAAGATGACTTTGCCGACTTCCACTCCAGTAAATTCTCATCCACG AGCTCAGACAAATCCCTGGGAGAGAAAGCCGTGGCTTTCAGACATGCCAAAGAAGACTCCTCCTCGGTGA AGTCTTTAGACCTCCCCTCCATCGGTGGCAGCAGTGTTGGCAAGGAGGACTCTGAAGATGCACTTTCTGT TCAATTTGACATGAAATTGGCTGATGTGGGAGGAGATCTTAAGCATGTCATGTCTGATAGCTCTTTGGAT TTACCAACAGTTAGTGGCCAGCATCCTCCTGCCGCAGGATCGGCCTTGGCCAGTGAGGACGCTCTTCCAG AGACTGCCTTCCCAGCTTTTGCCAGCTTTAAAGACATGATGCCTCAGACCACTGAGCAGAAAGAGTTTGA AAGTGGGGACTTCCAGGATTTCACCAGGCAGGACATGCCCACGGTGGACCGCAGCCAAGAAACCTCATGT CCAAGCCCGGCTTCCAGTGTGGCTTCGCACGAGACCCCCAAGGAGGGTGCAGACGACTTTGGAGAATTTC AAAGTGAAAAGTCCAAAATCAGCAAGTTTGACTTCTTAGTAGCTAATTCCCAGAGCAAAATGAAATCCAG TGAAGAAATGATCAAAAGTGAGCTGGCAACCTTTGACCTTTCTGTTCAAGGATCACACAAGAGGAGCTTG AGCCTTGGGGATAAAGAAATAAGCCGGTCCTCTCCTTCCCCGGCCCTGGAGCAGCCCTTCAGAGACCGGT CCAACACTCTGAGTGAGAGGGCTGCGCTGCCCGTCATCCGAGACAAGTACAAGGATCTCACTGGCGAGGT GGAGGAAAATGAGAGATACGCGTATGAGTGGCAGAGATGCCTAGGGAGCGCGCTGGATGTCATTAAGAAG GCAAATGACACCTTAAATGGCATTAGCAGCAGTGCTGTTTGCACAGAAGTTATCCAGTCGGCTCAAGGCA TGGAGTATCTTCTAGGTGTGGTGGAAGTGTACCGCGTAACCAAGCGTGTGGAGCTGGGGATAAAAGCCAC TGCTGTGTGTAGTGAGAAACTCCAGCAGTTGCTGAAGGACATCGATAAAGTGTGGAACAACTTAATCGGC TTCATGTCACTTGCCACACTCACACCAGATGAGAATTCACTGGATTTTTCCTCCTGTATGTTACGGCCCG GGATCAAAAACGCTCAGGAGCTGGCTTGTGGAGTGTGTCTCTTAAATGTGGACTCCAGGAGCCGGGCATT CAACTCGGAGACAGACAGCTTCAAGCTGGCCTACGGAGGACACCAGTACCACGCCAGCTGTGCCAACTTC TGGATCAACTGTGTTGAACCCAAGCCTCCCGGCCTCCTCTTGCCAGATCTGCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR211695 representing NM_194341 Red=Cloning site Green=Tags(s) MALRPGAGASGAAGAGAGPGGAGSFMFPVAGGMRPPQGLIPMQQQGFPMVSVMQPNMQGMMGMNYSSQMS QGPIAMQAGIPMGPMPAAGVPFLGQPPFLSMRPAGPQYTPDMQKQFAEEQQKRFEQQQKLLEEERKRRQF EEQKQKLRLLSSVKPKTGEKNRDDALEAIKGNLDGFSRDAKMHPTPASHPKKQGVGVFPSQDPAQSRMPP WIYNESLVPDAYKKILETTMTPTGIDTAKLYPILMSSGLPRETLGQIWALANRTTPGRLTKEELYTVLAM VAVTQRGVPAMSPDALSQFPAAPIPTLSGFPMTLPTPVSQPTAMPSGPTGSMPLTLGQPIMGINLVGPVG GAAAPTSSGFMPAYPSNQVGKTEEDDFQDFQDASKSGSIDDSFTDFQEMPASSKTSNSQHGNSAPSLLIP FPGTKASTDKYAVFKGISTDKPSENPASFGESGDKYSAFRELEQTTDSKPLGESFAEFRSTGTDDGFTDF KTADSVSPLEPPTKDTFPSAFASGAAQQTQTQVKTPLNLEDLDMFSSVDCSGEKQVPFSATFSTAKSVST RPQPAGSAAASAALASTKTSSLADDFGEFNLFGEYSNPASAGEQDDFADFMAFGNSSISSEPKASDKYEA LREEVSPSPLSSSTVEGAQHPPAAATKYDVFKQLSLEGAGLAMEEFKENTSSTKSEDDFADFHSSKFSST SSDKSLGEKAVAFRHAKEDSSSVKSLDLPSIGGSSVGKEDSEDALSVQFDMKLADVGGDLKHVMSDSSLD LPTVSGQHPPAAGSALASEDALPETAFPAFASFKDMMPQTTEQKEFESGDFQDFTRQDMPTVDRSQETSC PSPASSVASHETPKEGADDFGEFQSEKSKISKFDFLVANSQSKMKSSEEMIKSELATFDLSVQGSHKRSL SLGDKEISRSSPSPALEQPFRDRSNTLSERAALPVIRDKYKDLTGEVEENERYAYEWQRCLGSALDVIKK ANDTLNGISSSAVCTEVIQSAQGMEYLLGVVEVYRVTKRVELGIKATAVCSEKLQQLLKDIDKVWNNLIG FMSLATLTPDENSLDFSSCMLRPGIKNAQELACGVCLLNVDSRSRAFNSETDSFKLAYGGHQYHASCANF WINCVEPKPPGLLLPDLL SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_194341
ORF Size	3414 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_194341.2
RefSeq Size	4589 bp
RefSeq ORF	3417 bp
Locus ID	217030
UniProt ID	Q5SV85
MW	122.5 kDa
Gene Summary	May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins (By similarity).[UniProtKB/Swiss-Prot Function]

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