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Arhgef18 (NM_133962) Mouse Tagged ORF Clone
CAT＃: MR211463
- TrueORF®
Arhgef18 (Myc-DDK-tagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 18 (Arhgef18)
ORF Plasmid: tGFP
"NM_133962" in other vectors (3)
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CNY 7,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI467246; D030053O22Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211463 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATCTCTCAGAAAGGGGGTCTCCAGCCAACACCGAGCCCAGCTGGATCTGGGGTGCGACTTGGAC CAATTGCTGGAGACATGGATGAAGCTGACTCCGTGTTTTTAAAGTTGAAGCAGACGGCAGACGACTCTCT GTCACTCACATCCTCGAATGCTGAGTCTGTTTTCATAGAAGACCCTTACATTGCTTCACTGAGGTGTGAG ATCGAATCAGATGCACATGAGTTTGAGGCCGAGTCCTGGAGCCTCTCTGTGGATTTAGCATATGCCAAGA AACAGAAGAAGGAGGTGGTAAAGAGACAGGACGTCCTATATGAGCTGATGCAGACAGAGGCTCATCACGT GCGCACACTAAAGATCATGCTGAAAGTATACTCCAGAGCCCTGCAGGAAGAGCTGCAATTCAGTGGCCAG GCAGTCAGCCGTCTGTTTCCGTGCGCTGATGACTTACTGGACATGCACAGCCATTTCCTTGCCCGCCTTA AGGAGCGCCGGCAGGAGTTCCTAGAGGAGGGCAGTGATCGGAATTACGTCATCCAAAAGATCGGAGACGT CCTGGTTCAGCAGTTCTCAGGAGAAACCGGGGAGAGAATGAAGGAGAAATACGCTGTGTTCTGTAGTGGC CACAATGATGCAGTCGGTCATTACAAGCTATTGCTGCAGCAAAGCAAGAAGTTTCAAAACTTAATCAAGA AAATTGGCAACTTTTCCATTGTGCGAAGGCTGGGAGTGCAGGAGTGCATCCTTCTGGTCACACAGCGCAT AACCAAGTACCCGGTGCTTGTGGAACGCATCATCCAGAACACAGAAGCTGGCACTGAGGACTACAAGGAC CTGAGCCAAGCCCTGAGCCTCATCAAAGACATCATCTCACAAGTGGACGCCAAAGTCAGTGAGTATGAGA AGGACCAGCGCCTCAAGGAGATTGCAGCCAAGACGGACCAGAAGTCGTCTGGCAAACTCAAAAACGGGCT AACCTTCCGAAAGGAGGACATGCTGCAGCAGCGCCAGCTCCACCTGGAAGGCGCCCTCTGCTGGAAGTCC ACCTCAGGGCGCCTGAAAGATGTCCTCGCTGTCCTGCTGACCGATGTGCTTTTGCTGCTGCAAGAAAAAG ATCAGAAATATGTCTTTGCTTCTGTGGACTCAAAGCCACCCGTCATCTCTCTGCAAAAGCTCATCGTGAG AGAGGTAGCCAACGAGGAGAAGGCCATGTTCCTGATCAGCGCCTCCATGCAGGGGCCAGAGATGTATGAG ATGTACACCAGCTCCAAAGAGGACAGGAACATCTGGATGGCCCACATCCGCCGGGCCGTGGAGAGCTGTC CTGACGAGGAGGAGGACGTTTTCAGTGAGGCTGAGGAGAAAAAGATAGCTGAAGCCCGCACCATGAAACT CCAGGAATTTCAAGAGCGACTGAGCCTGAAAGACCAGCTGATTGCCCAGAGCCTTCTGGAGAAACAGCAG ATCTACCTGGAGATGGCTCAGCTGAGTGGGCTGGAAGAGTCAGCCCAGAACCGAGGCCTCTTCCGAGGAG GAGGAGACCCCTCTGAGACCCTGCGGGGGGAGCAGATTCTCAGATCGGCCATGAGTGAGATTGAGGGCAT CCAGAGCCTGATCTGCCAGCGGCACCTGGGCAGCACCAGTAGCCAAGTGGAGGAGGGAAGTGTCTCTGCA GGCCTGCCTCGGCGGGCTGAGACCTTCGGGGGCTATGACAGTGTGGGAAGCCCTAGCAAGGGTGGCAGCT TTAAAAGGAAGGTATCCAACAGTGACCTCAGGCCCCAAGACTGGCAGGGACCTGCTAGCAGCCCAGACTC CAGGCCCTGTGACAACAGCGCCCCCAGTGGCTGCTGTGAGGAATCGCCACAGGCTGTGGAGATGCCTAGC ACAGAGAGCCTGCCCACTGTCCTGGAATTAGAGCTGGTCCACCGGGTCCAGACACTGTCGCAGTTGCTTC TTAGTCTCCAGGCGGTCATCGCTCAACAGGATAGCTATGTGGAGATGCAAAGAACGGCCATCCAGGAGCG CGAGAAGCAGTTCCGGCTGCAGTCAACACGTGGGAACCTGCTGCTGGAGCAAGAGCGGCAACGCAACTTT GAGAAGCAGCGTGAGGAGCGCGCCGGTGTGGAGAAGCTGCAAAGCCAACTTCGCCAGGAGCAGCAGCGAT GGGAGCGGGAGCGGGCACGCCAGCAGCAGGAACTGGAGCTGGCCGGTGCGCGACTGCAGGAGCGAGAGGG CGAGGCACGCCAGATGCGCCAGCGGCTGGACCAGGAGCGCACCGAGCTAGAGCGACAGCGCCAGGCCTAC CAGCATGACCTGGAGCGTCTGCGTGAGGCCCAGCGAGCAGTGGACCGTGAACGTGAGCGCCTGGAGCTGC TGCGCAGGTTCAAGAAACAGAACACTGTACCAGGGGCACTGCCCCCGGAGGTGCTGGCAGAGGCCCAACC TGCAAGCCACCCTCCCAGCTTCAATGGCGATGGGCTGGAGGGGCACTCGGCCCCAGCCAAAGCACCAGGA ACCCAAGGGAGCGCGATGCTACATGGCACAGGACCTGACAACGTAGAGCGACCCGAGGTGGCCCGCTGGG ACAGTGCCCCCCCTGAGAGCCGGCCAGCCAAGAGCGATGTGCCCATCCAGCTACTCAGTGCCACCAATCA GATTCAGAGGCAGACAGCCGTGCAACAGCAGATCCCCACCAAGCTGGCCGCCTCCACCAAGGGTGGCAAG GAGAAGGGCAGCAAGAGCAGGGGTTCCCAGCGTTGGGAGAGCTCAGCGTCCTTCGACCTGAAGCAGCAGC TGCTCCTCAGCAAGTTCATAGGCAAGGATGAGAGTGCATCACGGAACCGCCGTTCACTGAGTCCCGTCCT GCCTGCCGCCCATGGGTCTGCACCTGCCTCAGACCCCTGCTTCCCAGCCCCAAGCCCGGCCCCAGCTGCC ACACCCCCCGAGGCCTTCAAGTTCGGAGGCACATCACTACCACCTGTCTCCCCAGCTTCCTCGCTGCCAA CCACACCACTCGCCACCACAGATGAAGTTAGTAAGGAAGATGTCATCTTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211463 protein sequence
Red=Cloning site Green=Tags(s) MTISQKGGLQPTPSPAGSGVRLGPIAGDMDEADSVFLKLKQTADDSLSLTSSNAESVFIEDPYIASLRCE IESDAHEFEAESWSLSVDLAYAKKQKKEVVKRQDVLYELMQTEAHHVRTLKIMLKVYSRALQEELQFSGQ AVSRLFPCADDLLDMHSHFLARLKERRQEFLEEGSDRNYVIQKIGDVLVQQFSGETGERMKEKYAVFCSG HNDAVGHYKLLLQQSKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDYKD LSQALSLIKDIISQVDAKVSEYEKDQRLKEIAAKTDQKSSGKLKNGLTFRKEDMLQQRQLHLEGALCWKS TSGRLKDVLAVLLTDVLLLLQEKDQKYVFASVDSKPPVISLQKLIVREVANEEKAMFLISASMQGPEMYE MYTSSKEDRNIWMAHIRRAVESCPDEEEDVFSEAEEKKIAEARTMKLQEFQERLSLKDQLIAQSLLEKQQ IYLEMAQLSGLEESAQNRGLFRGGGDPSETLRGEQILRSAMSEIEGIQSLICQRHLGSTSSQVEEGSVSA GLPRRAETFGGYDSVGSPSKGGSFKRKVSNSDLRPQDWQGPASSPDSRPCDNSAPSGCCEESPQAVEMPS TESLPTVLELELVHRVQTLSQLLLSLQAVIAQQDSYVEMQRTAIQEREKQFRLQSTRGNLLLEQERQRNF EKQREERAGVEKLQSQLRQEQQRWERERARQQQELELAGARLQEREGEARQMRQRLDQERTELERQRQAY QHDLERLREAQRAVDRERERLELLRRFKKQNTVPGALPPEVLAEAQPASHPPSFNGDGLEGHSAPAKAPG TQGSAMLHGTGPDNVERPEVARWDSAPPESRPAKSDVPIQLLSATNQIQRQTAVQQQIPTKLAASTKGGK EKGSKSRGSQRWESSASFDLKQQLLLSKFIGKDESASRNRRSLSPVLPAAHGSAPASDPCFPAPSPAPAA TPPEAFKFGGTSLPPVSPASSLPTTPLATTDEVSKEDVIFF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133962 |
| ORF Size | 3066 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133962.3, NP_598723.3 |
| RefSeq Size | 5300 bp |
| RefSeq ORF | 3066 bp |
| Locus ID | 102098 |
| UniProt ID | Q6P9R4 |
| MW | 114.3 kDa |
| Gene Summary | Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211463 | Arhgef18 (tGFP-tagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 18 (Arhgef18) |
CNY 9,790.00 |
|
| MR211463L3 | Lenti ORF clone of Arhgef18 (Myc-DDK-tagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 18 (Arhgef18) |
CNY 10,830.00 |
|
| MR211463L4 | Lenti ORF clone of Arhgef18 (mGFP-tagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 18 (Arhgef18) |
CNY 10,830.00 |
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