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Lig3 (NM_010716) Mouse Tagged ORF Clone
CAT＃: MR211442
- TrueORF®
Lig3 (Myc-DDK-tagged) - Mouse ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_010716" in other vectors (4)
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CNY 7,056.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D11Wsu78e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211442 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTTGGCTTTCAAGATCCTCTTCCCGAGAAACCTTTGTGCCCTTGGCAGAAAAGAACTGTGCCTGT TCCCAGAACAGAATCGCTGGGCTGCCATAAGTCAGTTCAGCCAGTGGTCGGAAACAAATCTCCTCGGTGG GTGCTGCCTTCTCCAGAGAAGAAAGCCTGTCCTAGCTCTCCAGAGAGGTCATCTAAGACCACGTGCCACC CACCTTACTTTCTGGCCAGGGTCGCATGTGGGACTCTGTACTGGCCCCTGTGCGATGGCAGAGCAGCGGT TCTGTGTGGACTATGCCAAGCGGGGCACAGCTGGATGCAAGAAATGCAAGGAGAAGATTGTAAAGGGCGT GTGCCGCATTGGCAAAGTGGTGCCCAACCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCAC ATTAAGTGCATGTTTGAGAAACTGGAACGGGCTCGGGCCACCACAAAGAAAATTGAAGACCTCACAGAGC TGGAAGGCTGGGAAGAGCTGGAAGATAACGAAAAGGAACAGATAAGCCAGCATATTGCAGACCTGTCTTC TAAGGCAGCTGCCACACCTAAGAAGAAAGCTGCTGTCCAGGCTAAGCTGACAACCACTGGCCAGGTGACA TCTCCAGTGAAAGGTGCTTCGTTTATTACCAGTACCAATCCTCGGAAGTTCTCTGGCTTTTCAGCAGCAA AACCCAACAACTCTGAGCAAGCCCCCTCAAGCCCTGCTCCTGGAACGAGTCTGTCTGCAAGTAAATGTGA CCCTAAGCACAAAGATTGTCTGCTACGAGAGTTCCGGAAGCTGTGCGCCATGGTGGCTGAAAATCCTAGC TATAACACCAAGACCCAGATCATCCATGACTTCTTGCAGAAAGGCTCCACAGGAGATGGCTTTCACGGTG ATGTGTACCTGACAGTTAAGCTGCTGCTGCCTGGTGTCATCAAGAGTGTTTACAACTTGAATGATAAGCA GATTGTAAAACTTTTTAGCCGAATTTTTAACTGCAACCCAGATGATATGGCCCGGGACCTAGAACAGGGT GACGTGTCAGAGACGATCAGAATCTTCTTTGAGCAGAGCAAGTCTTTCCCCCCAGCTGCCAAAAGCCTCC TCACCATTCAGGAAGTGGATGCCTTCCTCCTGCACCTCTCCAAGCTCACCAAAGAGGATGAGCAGCAGCA GGCCCTGCAGGACATAGCCTCCCGGTGTACAGCCAATGACCTTAAGTGCATTATTCGGCTGATCAAACAT GATCTGAAGATGAACTCGGGTGCAAAACACGTGTTAGATGCCCTTGACCCCAATGCTTATGAAGCCTTCA AAGCATCGCGCAACCTGCAGGATGTGGTGGAGCGAGTCCTTCACAATGAGCAGGAGGTGGAGAAGGACCC AGGCCGGCGACGGGCTCTGAGGGTCCAGGCCTCACTGATGACGCCTGTGCAGCCCATGCTGGCTGAGGCC TGCAAATCCATTGAATATGCAATGAAGAAGTGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGTG AGCGAGTCCAGGTGCATAAGAAGGGGGACCACTTCAGCTACTTCAGCCGCAGTCTCAAGCCTGTCCTGCC TCACAAGGTGGCCCACTTCAAGGACTATATACCCAAAGCTTTTCCTGGGGGTCAAAGCATGATCTTGGAT TCCGAGGTACTCCTGATTGACAACAACACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTGCACAAGA AAGCTGCTTTCCAGGATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTT GATGGACAGGCCTCTCTGTGAGCGACGGAAGTTTCTTCATGACAACATGGTTGAAATCCGTAACCGGATC ATGTTCTCAGAAATGAAGCAAGTCACAAAAGCTTCAGACCTGGCTGACATGATAAACCGGGTGATCCGAG AGGGCTTGGAAGGGTTGGTGCTAAAGGATGTGAAGGGTACATATGAGCCTGGGAAGCGGCACTGGCTGAA AGTTAAGAAAGATTATTTGAATGAGGGGGCCATGGCTGATACAGCTGATCTGGTGGTTCTTGGGGCCTTC TATGGGCAAGGGAGCAAAGGCGGAATGATGTCCATCTTCCTCATGGGCTGCTATGACCCTGACAGCCAAA AGTGGTGCACTGTTACAAAATGTGCTGGAGGCCATGATGATGCCACACTTGCCCGCCTGCAGAAAGAGCT GGTTATGGTGAAGATCAGCAAGGATCCTAGTAAGATACCCAGCTGGCTGAAGATCAACAAGATCTATTAT CCTGACTTTATCGTCCCAGACCCAAAGAAAGCTGCTGTGTGGGAGATCACAGGGGCAGAATTCTCCAGAT CTGAGGCACACACTGCTGATGGCATCTCCATCCGATTTCCTCGATGCACCCGAATCCGGGACGACAAGGA CTGGAAATCTGCCACTAACCTCCCCCAACTCAAGGAACTGTACCAGCTGTCCAAAGACAAGGCAGACTTT GCTGTGGTGGCTGGAGATGAGGCAAGCCCCACTACTGGAGGCAGCAGTGGCGAGAACGAGGGCACTGCTG GGTCTGCTGGGCCCTGCAAGGGCCCCCCCAGTAAGTCCTCTGCCAGTGCCAAGACCACCGAACAGAAGCT CAATAGCCCCAGTAGCAGAGGTGGCATCAAACCGATTCCAAAGCATTCCCCCATGAAACCAGGAGAGAAG CTGGCTGTGAAGTCTTCTCCAGTCAAAGTGGGAATGAAGAGGAAAGCTACTGATGAGACCCCATGCCTGA AAAAGGTACTGTTGGATGTCTTCACTGGGGTGCGGCTCTACTTGCCACCTTCTACACCAGACTTCAAACG TCTCAAACGCTACTTTGTGGCATTCGACGGGGACCTGGTACAGGAATTTGACATGGGCTCAGCCACACAT GTGCTAGGTAACAGGGAAAAGAACACTGATGCCCAGTTGGTCTCCTCAGAGTGGATTTGGGCATGTATCC GGAAACGGAGGCTGATAGCTCCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211442 protein sequence
Red=Cloning site Green=Tags(s) MTLAFKILFPRNLCALGRKELCLFPEQNRWAAISQFSQWSETNLLGGCCLLQRRKPVLALQRGHLRPRAT HLTFWPGSHVGLCTGPCAMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYH IKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKAAATPKKKAAVQAKLTTTGQVT SPVKGASFITSTNPRKFSGFSAAKPNNSEQAPSSPAPGTSLSASKCDPKHKDCLLREFRKLCAMVAENPS YNTKTQIIHDFLQKGSTGDGFHGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQG DVSETIRIFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKH DLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALRVQASLMTPVQPMLAEA CKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILD SEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRI MFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAF YGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELVMVKISKDPSKIPSWLKINKIYY PDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADF AVVAGDEASPTTGGSSGENEGTAGSAGPCKGPPSKSSASAKTTEQKLNSPSSRGGIKPIPKHSPMKPGEK LAVKSSPVKVGMKRKATDETPCLKKVLLDVFTGVRLYLPPSTPDFKRLKRYFVAFDGDLVQEFDMGSATH VLGNREKNTDAQLVSSEWIWACIRKRRLIAPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010716 |
| ORF Size | 3039 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010716.3 |
| RefSeq Size | 5988 bp |
| RefSeq ORF | 3039 bp |
| Locus ID | 16882 |
| MW | 112.8 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204874 | Lig3 (untagged) - Mouse ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,056.00 |
|
| MG211442 | Lig3 (tGFP-tagged) - Mouse ligase III, DNA, ATP-dependent (Lig3) |
CNY 9,220.00 |
|
| MR211442L3 | Lenti ORF clone of Lig3 (Myc-DDK-tagged) - Mouse ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein |
CNY 10,260.00 |
|
| MR211442L4 | Lenti ORF clone of Lig3 (mGFP-tagged) - Mouse ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein |
CNY 9,456.00 |
