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Sardh (NM_138665) Mouse Tagged ORF Clone
CAT＃: MR211181
- TrueORF®
Sardh (Myc-DDK-tagged) - Mouse sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_138665" in other vectors (4)
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CNY 6,728.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC6279 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211181 representing NM_138665
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCACTGAGCCGGGTCCTACGTGTAGCTGCCACCTGTCCTCGAGGGAGAGCTGCTTGGAATCTGG GGCTACAACCCCTAGCCACAGAGGCCAGGCCCACCACCGAGAAGAGTGTTCCTTATCAGCGGACCCTGAA GGAGGAGGCCCAAGGTGCCTCCGTGGTGCCCCAAGGACCAAGCCAGCCCCTGCCTAGCACAGCCAATGTT GTGGTCATCGGTGGGGGCAGTTTGGGCTGCCAGACTCTGTATCACTTGGCTAAGCTGGGTGTAGGTGGAG CCGTGCTGCTGGAGAGAGAGCGACTGACCTCTGGGACCACCTGGCACACAGCAGGCTTACTGTGGCAGCT GCGGCCCAGCGATGTGGAGGTAGAGCTTTTGGCTCACACACGGCAAGTGGTGAGCCGTGATCTGGAAGAG GAGACTGGGCTGCATACAGGCTGGATCCAGAATGGTGGCCTCTTCATCGCCTCTAACCAGCAACGCCTGA ATGAATACAAGAGGCTCATGTCGTTGGGCAAGGCCTATGGTATAGAATCCCACGTGCTGAGCCCAGCAGA GACCAAGAGTCTATATCCGCTGATGAACGTGGACGACCTCTATGGGACCCTGTATGTGCCACAGGATGGT ACCATGGACCCCGCTGGCACCTGTACTACCCTCACCAGAGCAGCAGTGGCTCGAGGAGCTCAGGTCATTG AAAACTGTGCGGTGACTGGCATCCGTGTGCGGACGGACGACTTTGGAGTGCGGCGGGTGGCAGCTGTGGA GACAGAGCATGGCTCCATCCAGACACCCTGTGTGGTCAACTGTGCAGGAGTGTGGGCGAGCAAGGTGGGC CGCATGGCTGGAGTGAAGGTCCCGCTGGTGGCCATGCACCATGCTTACGTCGTCACTGAGCGCATCGAGG GGATCCAGAACATGCCCAATGTCCGGGATCACGATGCTTCTGTCTACCTCCGACTGCAGGGGGATGCCTT GTCTGTGGGTGGCTATGAGGCCAACCCTATCTTTTGGGAGGAGGTGTCAGACAAATTTGCCTTTGGCCTC TTTGACCTGGACTGGGATGTGTTCACCCAGCACATTGAAGGCGCCATTAACCGTGTCCCTGTGTTGGAGA AGACGGGGATCAAGTCCACTGTCTGTGGTCCTGAATCCTTCACACCTGACCATAAGCCCCTGATGGGGGA GGCTCCTGAACTCCGTGGCTTCTTCCTTGGCTGTGGCTTCAACAGTGCAGGCATGATGTTGGGTGGTGGC TGTGGACAAGAGCTGGCCCATTGGATTGTGCACGGGCGCCCAGAGAAGGACATGTACAGCTATGACATCA GGCGTTTCCATCACTCGCTCACGGACCACACCCGCTGGATCCGAGAGCGCAGCCATGAGTCCTATGCCAA GAACTACTCGGTTGTCTTCCCACACGATGAGCCACTGGCAGGACGCAACATGAGGAGAGACCCCCTGCAT GAGGAGCTGCTTGGACAAGGCTGTGTGTTTCAAGAGCGACAGGGATGGGAACGGCCGGGATGGTTCAACC CCCAGGAAACAGCTCAGGTCCTTGACTACGACTACTACGGGGCCTACGGAAACCAGGCACACAAGGACTA CACCTACAGCAGGTTGCTAGGCGATGAGTACACCTTCGACTTCCCACCCCACCACCACATGATCCAGAAG GAATGTCTGGCCTGCAGAGGGGCTGCTGCTGTGTTCAACATGTCTTACTTTGGGAAGTTCTACCTGCTCG GGGTGGACGCCAGGAAGGCTGCAGACTGGCTCTTCTCAGCAGATGTCAACCGACCCCCAGGCTCAACAGT GTACACATGCATGCTGAACCAACGGGGAGGCACTGAGAGCGACCTGACTGTTAGCCGATTGGCTCCTGGC ACCCAGGCCAGTCCCCTGGTCCCTGCCTTTGAAGGGGATTGCTACTACCTGGCCGTGGGTGGGGCTGTGG CCCAACACAACTGGTCCCACATAAACACGGTGCTACAGGACCAGGAGTTCCGGTGCCAGCTTATGGATAG TTCTGAAGATCTGGGCATGCTCAGTATCCAGGGCCCAGCCAGCCGTGACATCCTGCAGGATGTGCTTGAT GCTGACCTGAGCAACGAGGCCTTCCCCTTCTCCACCCACCAGCTGGTGAGAGCCGCCGGGCACCTGGTCC GGGCCATCAGGCTGTCCTTTGTCGGGGAGCTGGGATGGGAGCTGCATGTACCCCGGGCATCCTGCTTGCC AGTGTACCGGGCTGTGATGGCTGCAGGTGCGAGGCATGGCCTAGTCAACGCAGGCTACCGTGCCATCGAC TCCCTGAGCATCGAGAAAGGCTATAGACACTGGCATGCAGACCTGAGGCCCGATGACAGCCCCCTGGAGG CAGGATTGGCCTTCACCTGCAAACTCAAGACCTCGGTGCCCTTCCTAGGCCGAGAGGCCCTGGAGAAGCA GCGTGCCACAGGTCTCCGCCGACGCCTGATATGCCTCACTGTGGAAGAGGAGGTGCCTATGTTTGGCCTG GAGGCCATCTGGAGAAACGGCCAAGTGGTGGGCCATGTCCGGAGAGCTGACTTTGGATTTACTGTGAACA AGACCATCGCCTATGGCTACATCCGAGACCCCAGTGGTGGCCCTGTCTCTCTGGACTTTGTGAAGAATGG AGAGTATGCCTTGGAGAGAATGGGGGTCACCTATGCTGCCCAGGTTCACCTGAAGTCTCCCTTTGACCCT GACAACAAGCGGGTGAAGGGAATCTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211181 representing NM_138665
Red=Cloning site Green=Tags(s) MASLSRVLRVAATCPRGRAAWNLGLQPLATEARPTTEKSVPYQRTLKEEAQGASVVPQGPSQPLPSTANV VVIGGGSLGCQTLYHLAKLGVGGAVLLERERLTSGTTWHTAGLLWQLRPSDVEVELLAHTRQVVSRDLEE ETGLHTGWIQNGGLFIASNQQRLNEYKRLMSLGKAYGIESHVLSPAETKSLYPLMNVDDLYGTLYVPQDG TMDPAGTCTTLTRAAVARGAQVIENCAVTGIRVRTDDFGVRRVAAVETEHGSIQTPCVVNCAGVWASKVG RMAGVKVPLVAMHHAYVVTERIEGIQNMPNVRDHDASVYLRLQGDALSVGGYEANPIFWEEVSDKFAFGL FDLDWDVFTQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLGGG CGQELAHWIVHGRPEKDMYSYDIRRFHHSLTDHTRWIRERSHESYAKNYSVVFPHDEPLAGRNMRRDPLH EELLGQGCVFQERQGWERPGWFNPQETAQVLDYDYYGAYGNQAHKDYTYSRLLGDEYTFDFPPHHHMIQK ECLACRGAAAVFNMSYFGKFYLLGVDARKAADWLFSADVNRPPGSTVYTCMLNQRGGTESDLTVSRLAPG TQASPLVPAFEGDCYYLAVGGAVAQHNWSHINTVLQDQEFRCQLMDSSEDLGMLSIQGPASRDILQDVLD ADLSNEAFPFSTHQLVRAAGHLVRAIRLSFVGELGWELHVPRASCLPVYRAVMAAGARHGLVNAGYRAID SLSIEKGYRHWHADLRPDDSPLEAGLAFTCKLKTSVPFLGREALEKQRATGLRRRLICLTVEEEVPMFGL EAIWRNGQVVGHVRRADFGFTVNKTIAYGYIRDPSGGPVSLDFVKNGEYALERMGVTYAAQVHLKSPFDP DNKRVKGIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138665 |
| ORF Size | 2757 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138665.2, NP_619606.1 |
| RefSeq Size | 4573 bp |
| RefSeq ORF | 2760 bp |
| Locus ID | 192166 |
| UniProt ID | Q99LB7 |
| MW | 101.7 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200450 | Sardh (untagged) - Mouse sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MG211181 | Sardh (tGFP-tagged) - Mouse sarcosine dehydrogenase (Sardh) |
CNY 7,890.00 |
|
| MR211181L3 | Lenti ORF clone of Sardh (Myc-DDK-tagged) - Mouse sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein |
CNY 9,030.00 |
|
| MR211181L4 | Lenti ORF clone of Sardh (mGFP-tagged) - Mouse sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein |
CNY 9,030.00 |
