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Sort1 (NM_019972) Mouse Tagged ORF Clone
CAT＃: MR210834
- TrueORF®
Sort1 (Myc-DDK-tagged) - Mouse sortilin 1 (Sort1)
ORF Plasmid: tGFP
"NM_019972" in other vectors (4)
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CNY 9,064.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2900053A11Rik; AI852375; Ntr3; Ntsr3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210834 representing NM_019972
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGCCCCGGGGAGCTGCGGACGGCCTTTTGCGCTGGCCCCTCGGCCTCCTCCTGCTCCTTCAAC TGCTGCCTCCTGCCGCCGTCGGCCAGGACCGGCTGGACGCGCCGCCGCCGCCCGCGCCTCCTCTGCTGCG CTGGGCCGGTCCGGTCGGGGTGAGCTGGGGGCTGCGCGCCGCCGCGCCCGGGGGCCCCGTCCCCCGCGCT GGCCGTTGGCGCCGCGGCGCGCCCGCCGAGGACCAAGACTGCGGCCGCCTCCCGGACTTCATCGCCAAGC TGACCAACAATACGCACCAGCATGTCTTTGATGACCTCAGTGGCTCAGTGTCCTTGTCCTGGGTTGGAGA CAGCACTGGGGTTATTCTCGTCCTGACCACTTTCCAAGTGCCTCTGGTAATTGTGAGCTTTGGACAGTCC AAGTTGTATCGAAGTGAGGATTATGGAAAGAACTTTAAGGATATTACAAATCTCATCAATAACACCTTCA TTCGGACGGAATTTGGCATGGCTATTGGTCCTGAGAACTCTGGAAAGGTGATACTAACAGCGGAGGTGTC CGGGGGAAGCCGAGGCGGAAGAGTGTTCAGGTCATCAGACTTTGCCAAGAACTTTGTGCAAACAGATCTC CCCTTTCATCCTCTGACGCAGATGATGTACAGCCCTCAGAATTCTGATTACCTGTTAGCTCTCAGCACCG AAAATGGCCTGTGGGTGTCCAAGAATTTTGGGGAAAAATGGGAAGAAATCCACAAAGCAGTATGTTTGGC CAAATGGGGACCAAACAACATCATCTTCTTTACCACCCATGTGAATGGCTCCTGCAAAGCTGATCTTGGT GCCCTGGAATTATGGAGAACATCCGACTTGGGAAAAACCTTCAAAACCATTGGTGTGAAAATCTACTCCT TTGGTCTTGGGGGCCGTTTCCTTTTTGCCTCTGTGATGGCTGATAAGGACACAACAAGAAGGATCCATGT GTCAACAGACCAGGGGGACACATGGAGCATGGCACAACTTCCTTCTGTGGGACAGGAACAGTTCTACTCC ATCCTGGCAGCCAATGAGGACATGGTCTTCATGCATGTAGATGAACCTGGAGATACCGGGTTTGGCACCA TCTTTACCTCTGATGATCGAGGCATTGTCTACTCCAAGTCTCTGGACAGACATCTCTATACCACCACAGG CGGGGAGACGGACTTTACCAACGTGACTTCCCTCCGTGGGGTCTATATAACAAGCACGCTCTCAGAAGAT AACTCTATTCAGAGCATGATCACTTTTGACCAGGGAGGACGGTGGGAGCACCTGCGGAAGCCGGAGAACA GCAAGTGCGACGCTACCGCAAAGAACAAGAACGAGTGCAGCCTTCATATCCATGCTTCTTATAGCATCTC CCAGAAGCTAAACGTTCCAATGGCCCCACTTTCCGAGCCCAATGCTGTGGGCATAGTCATCGCTCACGGT AGTGTGGGAGATGCCATCTCGGTGATGGTCCCAGATGTGTACATCTCAGATGATGGGGGTTACTCCTGGG CGAAGATGCTAGAAGGACCACATTACTATACCATCCTGGACTCTGGAGGCATCATTGTGGCCATTGAGCA CAGCAACCGTCCTATCAATGTGATTAAGTTCTCCACAGATGAAGGCCAGTGCTGGCAGAGCTATGTGTTC ACACAGGAGCCCATCTACTTCACTGGGCTTGCTTCCGAGCCTGGAGCCAGGTCCATGAACATCAGCATCT GGGGATTCACAGAGTCTTTCATTACCCGCCAGTGGGTCTCCTACACAGTCGATTTCAAAGACATCCTTGA GCGGAATTGTGAAGAGGATGACTATACCACGTGGCTGGCACACTCCACAGACCCTGGAGATTACAAAGAC GGCTGCATTTTGGGCTATAAAGAACAGTTCCTACGGCTACGGAAGTCATCCGTCTGTCAGAATGGTCGAG ACTATGTTGTGGCCAAGCAGCCATCCGTCTGTCCGTGTTCCCTGGAGGACTTCCTCTGTGACTTTGGCTA CTTCCGTCCGGAGAACGCCTCAGAGTGCGTGGAGCAGCCTGAACTGAAGGGGCATGAGTTAGAGTTCTGT CTGTACGGCAAGGAGGAGCACCTGACAACAAATGGGTACCGGAAAATCCCAGGAGACAAATGCCAAGGTG GGATGAATCCCGCCAGAGAAGTAAAAGACTTGAAAAAGAAATGCACAAGCAACTTCTTGAACCCCACAAA GCAGAATTCCAAGTCAAATTCTGTCCCTATTATCCTGGCCATCGTGGGACTGATGCTTGTCACAGTCGTA GCAGGAGTCCTCATTGTGAAGAAATATGTCTGTGGCGGAAGGTTCCTGGTGCACCGGTACTCGGTGCTAC AGCAGCACGCAGAGGCTGACGGCGTAGAGGCTTTGGATTCAACCTCCCACGCTAAAAGCGGATATCACGA CGACTCAGATGAGGACCTCCTGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210834 representing NM_019972
Red=Cloning site Green=Tags(s) MERPRGAADGLLRWPLGLLLLLQLLPPAAVGQDRLDAPPPPAPPLLRWAGPVGVSWGLRAAAPGGPVPRA GRWRRGAPAEDQDCGRLPDFIAKLTNNTHQHVFDDLSGSVSLSWVGDSTGVILVLTTFQVPLVIVSFGQS KLYRSEDYGKNFKDITNLINNTFIRTEFGMAIGPENSGKVILTAEVSGGSRGGRVFRSSDFAKNFVQTDL PFHPLTQMMYSPQNSDYLLALSTENGLWVSKNFGEKWEEIHKAVCLAKWGPNNIIFFTTHVNGSCKADLG ALELWRTSDLGKTFKTIGVKIYSFGLGGRFLFASVMADKDTTRRIHVSTDQGDTWSMAQLPSVGQEQFYS ILAANEDMVFMHVDEPGDTGFGTIFTSDDRGIVYSKSLDRHLYTTTGGETDFTNVTSLRGVYITSTLSED NSIQSMITFDQGGRWEHLRKPENSKCDATAKNKNECSLHIHASYSISQKLNVPMAPLSEPNAVGIVIAHG SVGDAISVMVPDVYISDDGGYSWAKMLEGPHYYTILDSGGIIVAIEHSNRPINVIKFSTDEGQCWQSYVF TQEPIYFTGLASEPGARSMNISIWGFTESFITRQWVSYTVDFKDILERNCEEDDYTTWLAHSTDPGDYKD GCILGYKEQFLRLRKSSVCQNGRDYVVAKQPSVCPCSLEDFLCDFGYFRPENASECVEQPELKGHELEFC LYGKEEHLTTNGYRKIPGDKCQGGMNPAREVKDLKKKCTSNFLNPTKQNSKSNSVPIILAIVGLMLVTVV AGVLIVKKYVCGGRFLVHRYSVLQQHAEADGVEALDSTSHAKSGYHDDSDEDLLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019972 |
| ORF Size | 2475 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019972.3, NP_064356.2 |
| RefSeq Size | 6851 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 20661 |
| UniProt ID | Q6PHU5 |
| MW | 91.7 kDa |
| Gene Summary | Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Sortilin, Encoded by the Cardiovascular Risk Gene SORT1, and Its Suggested Functions in Cardiovascular Disease
,Kjolby, M;Nielsen, MS;Petersen, CM;,
Curr Atheroscler Rep
,PubMed ID 25702058
[Sort1]
|
|
Hepatic sortilin regulates both apolipoprotein B secretion and LDL catabolism
,null,
The Journal of Clinical Investigation
,PubMed ID 22751103
[Sort1]
|
|
From noncoding variant to phenotype via SORT1 at the 1p13 cholesterol locus
,Kiran Musunuru, Alanna Strong, Maria Frank-Kamenetsky, Noemi E. Lee, Tim Ahfeldt, Katherine V. Sachs, Xiaoyu Li, Hui Li, Nicolas Kuperwasser, Vera M. Ruda, et al.,
Nature 466, 714-719 (5 August 2010) doi:10.1038/nature09266 Article
[SORT1]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221836 | Sort1 (untagged) - Mouse sortilin 1 (Sort1), (10ug) |
CNY 9,072.00 |
|
| MG210834 | Sort1 (tGFP-tagged) - Mouse sortilin 1 (Sort1) |
CNY 10,664.00 |
|
| MR210834L3 | Lenti ORF clone of Sort1 (Myc-DDK-tagged) - Mouse sortilin 1 (Sort1) |
CNY 10,170.00 |
|
| MR210834L4 | Lenti ORF clone of Sort1 (mGFP-tagged) - Mouse sortilin 1 (Sort1) |
CNY 11,464.00 |
