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Sema3e (NM_011348) Mouse Tagged ORF Clone
CAT＃: MR210592
- TrueORF®
Sema3e (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e)
ORF Plasmid: tGFP
"NM_011348" in other vectors (4)
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CNY 5,680.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6430702L12; AA408817; mKIAA0331; Semah |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210592 representing NM_011348
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCGGCCGGACACATCCTCACCTTGCTGCTCTGGGGTCACCTGCTGGAACTCTGGACCCCAGGTC ACTCCGCGAACCCCTCCTACCCCAGGCTACGCCTGTCACATAAAGAACTTTTGGAACTGAATAGGACTTC AATATTTCAAAGCCCCCTTGGATTTCTTGATCTCCATACAATGCTGCTGGATGAGTATCAAGAACGGCTC TTTGTGGGAGGCAGAGACCTTGTCTATTCCCTGAACTTGGAACGAGTCAGTGACGGCTACAGAGAGATAT ACTGGCCGAGCACAGCAGTAAAGGTAGAAGAATGCATAATGAAAGGAAAAGACGCAAATGAGTGTGCCAA TTATATCCGGGTTTTGCATCACTACAACAGGACACACCTTCTGACCTGTGCTACTGGAGCTTTTGATCCA CACTGTGCCTTCATCAGAGTCGGGCACCATTCAGAGGAACCCCTGTTTCACCTGGAGTCACACAGATCTG AGAGAGGAAGGGGCAGATGTCCTTTTGACCCCAACTCCTCCTTTGTGTCCACGCTAGTTGGGAATGAGCT GTTTGCTGGACTCTACAGTGACTATTGGGGCAGAGACTCGGCGATCTTCCGCAGCATGGGGAAGTTAGGC CATATTCGCACTGAGCATGACGATGAGCGGCTCCTGAAAGAACCAAAATTTGTAGGTTCATATATGATTC CTGATAACGAAGACCGAGATGACAACAAAATGTACTTTTTCTTTACTGAGAAGGCGCTGGAGGCGGAGAA CAACGCCCACACGATCTACACCCGAGTGGGGCGGCTGTGCGTGAATGACATGGGAGGACAGAGAATCCTG GTGAACAAGTGGAGCACTTTCCTTAAAGCGCGGCTGGTTTGCTCAGTGCCGGGAATGAATGGAATCGACA CATACTTTGACGAACTAGAGGATGTGTTTTTACTGCCGACCAGAGATCCTAAGAATCCAGTGATATTTGG ACTGTTTAATACTACCAGCAATATATTTAGAGGCCATGCTGTATGTGTGTATCACATGTCAAGTATCCGG GAAGCCTTTAATGGCCCATATGCTCATAAAGAAGGCCCTGAATACCACTGGTCACTATATGAAGGAAAAG TCCCCTACCCAAGGCCTGGTTCCTGTGCCAGCAAAGTAAACGGAGGCAAGTATGGAACCACCAAAGATTA CCCCGATGACGCCATCCGGTTCGCAAGGATGCATCCTCTAATGTATCAGCCCATAAAACCTGTTCATAAA AAACCAATACTGGTAAAAACAGATGGAAAATACAACCTGAGGCAACTTGCCGTGGATCGGGTGGAAGCGG AGGATGGCCAGTATGACGTCTTATTTATTGGGACAGACACAGGAATTGTGCTGAAAGTAATCACAATTTA CAACCAAGAAACAGAGTGGATGGAGGAAGTCATTCTAGAGGAACTTCAAATATTCAAGGATCCAGCCCCT ATCATTTCTATGGAAATTTCTTCAAAGAGACAACAGCTTTACATTGGATCAGCCTCTGCTGTGGCACAAG TCAGATTCCATCACTGCGACATGTATGGCAGTGCTTGTGCTGACTGCTGCCTGGCTCGAGACCCGTACTG TGCCTGGGATGGCATATCCTGCTCCAGGTACTACCCAACAGGTGCACACGCAAAGAGGAGGTTCCGCAGG CAGGACGTTCGGCATGGCAACGCCGCCCAACAGTGCTTTGGACAGCAATTTGTTGGAGACGCGTTGGACA GGACTGAAGAGAGGCTGGCTTATGGCATAGAGAGCAACAGTACTCTGTTGGAATGCACCCCGCGATCACT ACAAGCAAAAGTCATCTGGTTTGTACAGAAGGGACGCGACGTAAGAAAAGAAGAGGTGAAGACGGATGAC AGAGTTGTCAAGATGGACTTGGGCTTGCTCTTCCTCAGAGTACGCAAGTCAGATGCAGGGACCTATTTTT GCCAGACAGTAGAACACAATTTTGTCCATACTGTGCGTAAAATCACCTTGGAGGTGGTCGAAGAGCATAA AGTGGAGGGCATGTTTCATAAGGACCATGAAGAGGAAAGACATCACAAGATGCCCTGCCCTCCCTTAAGC GGTATGTCTCAGGGGACAAAACCGTGGTACAAGGAATTCTTGCAGCTGATTGGCTACAGCAACTTCCAGA GAGTGGAAGAATACTGCGAAAAGGTGTGGTGTACAGATAAGAAGAGGAAAAAGCTTAAAATGTCTCCCTC CAAGTGGAAGTATGCCAACCCCCAGGAAAAGAGGCTTCGCTCTAAAGCTGAGCACTTCCGCCTGCCCAGG CACACGCTGCTCTCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR210592 representing NM_011348
Red=Cloning site Green=Tags(s) MAPAGHILTLLLWGHLLELWTPGHSANPSYPRLRLSHKELLELNRTSIFQSPLGFLDLHTMLLDEYQERL FVGGRDLVYSLNLERVSDGYREIYWPSTAVKVEECIMKGKDANECANYIRVLHHYNRTHLLTCATGAFDP HCAFIRVGHHSEEPLFHLESHRSERGRGRCPFDPNSSFVSTLVGNELFAGLYSDYWGRDSAIFRSMGKLG HIRTEHDDERLLKEPKFVGSYMIPDNEDRDDNKMYFFFTEKALEAENNAHTIYTRVGRLCVNDMGGQRIL VNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDPKNPVIFGLFNTTSNIFRGHAVCVYHMSSIR EAFNGPYAHKEGPEYHWSLYEGKVPYPRPGSCASKVNGGKYGTTKDYPDDAIRFARMHPLMYQPIKPVHK KPILVKTDGKYNLRQLAVDRVEAEDGQYDVLFIGTDTGIVLKVITIYNQETEWMEEVILEELQIFKDPAP IISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGAHAKRRFRR QDVRHGNAAQQCFGQQFVGDALDRTEERLAYGIESNSTLLECTPRSLQAKVIWFVQKGRDVRKEEVKTDD RVVKMDLGLLFLRVRKSDAGTYFCQTVEHNFVHTVRKITLEVVEEHKVEGMFHKDHEEERHHKMPCPPLS GMSQGTKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDKKRKKLKMSPSKWKYANPQEKRLRSKAEHFRLPR HTLLS SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011348 |
| ORF Size | 2325 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011348.2, NP_035478.2 |
| RefSeq Size | 6877 bp |
| RefSeq ORF | 2328 bp |
| Locus ID | 20349 |
| UniProt ID | P70275 |
| MW | 90 kDa |
| Gene Summary | Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203244 | Sema3e (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e), (10ug) |
CNY 2,000.00 |
|
| MG210592 | Sema3e (tGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e) |
CNY 6,370.00 |
|
| MR210592L3 | Lenti ORF clone of Sema3e (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e) |
CNY 7,700.00 |
|
| MR210592L4 | Lenti ORF clone of Sema3e (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e) |
CNY 7,700.00 |
