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Amph (NM_175007) Mouse Tagged ORF Clone
CAT＃: MR210042
- TrueORF®
Amph (Myc-DDK-tagged) - Mouse amphiphysin (Amph)
ORF Plasmid: tGFP
"NM_175007" in other vectors (4)
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CNY 5,024.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC49429 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210042 representing NM_175007
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACATCAAGACGGGCATCTTCGCCAAAAACGTGCAAAAGCGCCTCAACCGCGCGCAGGAAAAGG TCCTCCAGAAGCTGGGGAAGGCTGACGAGACAAAAGATGAACAGTTTGAAGAATATGTCCAGAACTTCAA ACGGCAGGAGGCAGAGGGTACCAGACTTCAGCGAGAACTCCGGGGATATTTAGCAGCAATCAAAGGTATG CAGGAGGCCTCAATGAAGCTCACTGAATCTCTACATGAAGTCTACGAGCCAGACTGGTATGGACGGGAAG ATGTAAAGATGGTTGGTGAGAAGTGTGATGTGCTGTGGGAAGACTTCCATCAAAAGCTAGTGGATGGCTC CTTGCTGACACTGGACACCTACCTGGGCCAGTTTCCTGACATAAAGAATCGCATCGCTAAGCGCAGCAGG AAGCTTGTGGATTATGATAGTGCTCGCCATCACCTGGAGGCTCTGCAGAGTTCCAAACGGAAAGATGAGA GTCGGATCTCTAAGGCAGAAGAGGAGTTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTCAATGTTGACTT ACAAGAAGAGTTGCCGTCATTGTGGTCAAGTCGAGTTGGCTTTTACGTCAATACTTTCAAAAATGTCTCC AGCCTTGAGGCCAAGTTTCATAAGGAAATCGCAGTGCTTTGCCACAAGCTCTATGAAGTGATGACGAAAC TCGGTGATCAGCATGCTGACAAGGCCTTCAGCATTCAAGGAGCTCCCAGTGACTCGGGTCCTCTTCGCAT TGCAAAGACACCATCACCTCCAGAGGAGCCTTCTCCCCTGCCCAGCCCCACGGCTAGTCCCAACCACACA TTAGCACCTGCATCTCCTGCCCCAGTGCGACCCAGATCACCTTCACAGACAAGGAAAGGGCCTCCTGTCC CACCTCTGCCTAAAGTCACCCCAACAAAGGAACTGAAACAGGAGAACATCATCAATTTCTTTGAGGACAA CTTTGTACCAGAAATCAATGTGACAACACCTTCCCAGAACGAAGTCTTGGAGGTGAAGAAGGAAGAGACT TTGCTGGATTTGGACTTTGACCCTTTCAAGCCCGATGTGGCTCCGGCAGGTTCTGCTGCAGCGACCCACT CTCCCATGTCTCAGACATTGCCCTGGGACTTATGGACGACAAGCACTGATTTGGTACAACCAGCTTCTGG GGGTTCATTTAATGATTTCACCCAGGCCCAGGACACTTCATTATTCACCATGCAGACAGATCAGAATATG GCTGAAACTGAGCAGGCTCTACCTACAGAGCCCCAAGCCGAAGAGCCTCCTGCCACTGCTGCTGCACCTA CTGCTGGGCTGGACCTTGGGCTAGAGATGGAGGAGCCAAAGGAGGAGGCTGTGATCCCTCCCGCAACTGA TACTGGCGAGACTGTGGAAACTGCAGTGCCAACTGAGGGTGCCCCGGTGGAGGAAGCAGAGGCAGAGAAG GCAGCCCTCCCTGCTGGAGAAGGAGGAAGTCCAGAGGGAGCCAAAATTGACGGGGAAAGCACGGAGCTTG CAATCAGTGAGAGCCCTCAACCAGTGGAGCCCGAAGCGGGTGCTCCTCAGGTCATCCCTTCTGTTGTCAT TGAGCCAGCCTCCAACCATGAAGGAGAAGGAGAACACCAAGAAACTGCTACGGGTACTGAACCGAGGGAG GCCGCTGAGGATGTGGCTGCCCAGGGCTCAGCGGGTGAGAAGCAGGAAGTGGCTACAGAGCCCACGCCTC TGGATTCTCAGGCAACTCTGCCTGCATCTGCAGGTGCTGTGGATGCCTCGCTGTCTGCAGGGGATGCCAC TCAGGAACTGCCTCCTGGCTTTCTCTACAAGGTGGAAACCCTGCATGATTTCGAGGCAGCGAACTCTGAT GAACTTAACCTACAAAGGGGTGACGTGGTACTCGTGGTGCCTTCAGACTCAGAAGCCGACCAGGATGCAG GCTGGTTGGTGGGGGTGAAGGAGTCAGACTGGCTTCAGTACAGAGACCTGGCCACGTACAAAGGCCTCTT TCCAGAGAACTTCACGCGGCGCCTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210042 representing NM_175007
Red=Cloning site Green=Tags(s) MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGM QEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSR KLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSSRVGFYVNTFKNVS SLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHT LAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELKQENIINFFEDNFVPEINVTTPSQNEVLEVKKEET LLDLDFDPFKPDVAPAGSAAATHSPMSQTLPWDLWTTSTDLVQPASGGSFNDFTQAQDTSLFTMQTDQNM AETEQALPTEPQAEEPPATAAAPTAGLDLGLEMEEPKEEAVIPPATDTGETVETAVPTEGAPVEEAEAEK AALPAGEGGSPEGAKIDGESTELAISESPQPVEPEAGAPQVIPSVVIEPASNHEGEGEHQETATGTEPRE AAEDVAAQGSAGEKQEVATEPTPLDSQATLPASAGAVDASLSAGDATQELPPGFLYKVETLHDFEAANSD ELNLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175007 |
| ORF Size | 2058 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175007.2, NP_778172.1 |
| RefSeq Size | 3221 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 218038 |
| UniProt ID | Q7TQF7 |
| MW | 75.5 kDa |
| Gene Summary | May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220459 | Amph (untagged) - Mouse amphiphysin (Amph), (10ug) |
CNY 8,740.00 |
|
| MG210042 | Amph (tGFP-tagged) - Mouse amphiphysin (Amph) |
CNY 6,370.00 |
|
| MR210042L3 | Lenti ORF clone of Amph (Myc-DDK-tagged) - Mouse amphiphysin (Amph) |
CNY 7,700.00 |
|
| MR210042L4 | Lenti ORF clone of Amph (mGFP-tagged) - Mouse amphiphysin (Amph) |
CNY 7,700.00 |
