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Dhx58 (NM_030150) Mouse Tagged ORF Clone
CAT＃: MR209973
- TrueORF®
Dhx58 (Myc-DDK-tagged) - Mouse DEXH (Asp-Glu-X-His) box polypeptide 58 (Dhx58)
ORF Plasmid: tGFP
"NM_030150" in other vectors (4)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B430001I08Rik; D11Lgp2e; Lgp2; LPG2; RLR-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209973 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCGACCCTACCAGTGGGAAGTGATCTTACCTGCTCTGGAGGGCAAGAATATCATTATATGGC TGCCCACGGGTGCTGGGAAGACCCGGGCAGCTGCCTTTGTAGCCAAGAGGCATCTAGAGACGGTAGACAG AGGCAAGGTGGTGGTACTGGTCAATAGGGTACACCTGGTGAGCCAGCACGCCGAGGAGTTCAGGCGCATG CTGGATAAACACTGGACCGTAACAACCCTGAGTGGGGACATGGGATCCCGAGCTGGCTTTGGCCTGATGG CTCGGAGCCACGACCTGCTCATCTGTACGGCAGAGTTGTTACAGTTGGCACTCAACAGCTCTGAGGAGGA TGAACACGTAGAGCTCAGAGAATTCTCGCTGATTGTGGTGGACGAGTGTCACCACACCCACAAGGACACC GTCTACAACACCATCTTGAGCCGGTACCTAGAACAGAAGCTGAAGAAGGCAGAGCCCCTCCCCCAGGTCC TGGGTCTCACAGCCTCCCCAGGCACTGGAGGGGCCACCAAGCTCCAAGGGGCCATTGATCACATCCTACA GCTTTGTGCGAATTTAGATACGTGCCACATCATGTCGCCAAAGAATTGCTACTCCCAGCTGCTGATGCAT AACCCGAAGCCCTGCAAGCAGTATGACCTCTGCCAAAGGCGCGCACAGGATCCTTTTGGGGACTTGATAA AAAAGCTTATGAACCAAATCCACCAACAACTAGAGATGCCTGACTTGAAGCAACAATTTGGAACCCAGAT GTATGAGCAGCAAGTGGTACAGTTGTGCAAGGATGCGGCAGAGGCTGGACTCCAGGAACAGCGGGTGTAT GCGCTGCATTTGCGGCGCTACAATGATGCGCTATTTATCCACGATACTGTCCGTGCCCGGGACGCCCTTG ACATGTTGCAAGATTTTTACGACAGAGAACGCACCACAAAAACACAGATGGTGCGTGCTGAAAGCTGGCT GCTGAAGCTGTTTGATGACCATAAAAATGTGCTGGGCCAGCTAGCAGCTCGGGGTCCTGAGAACCCGAAG TTGGAGATGCTGGAAAGGATCTTACTGAAGCAGTTTGGGAGTCCTGGCCACACTCGGGGTATCATCTTCA CCAGAACCCGTCAGACTGCTTCCTCCCTCCTGCTCTGGCTTCGGCAGCAGCCTTGCCTACAGACTGTGGG CATCAAGCCACAGATGCTGATCGGAGCAGGGAACACAAGCCAGAGCACACACATGACCCAGAAAGACCAG CAGGAAGTGATCCAGGAGTTCAGGGATGGTATCCTGAGCCTTCTAGTGGCCACAAGTGTGGCAGAGGAGG GGCTGGATATCGCTCAGTGCAATGTGGTGGTGCGCTATGGGCTCCTGACCAATGAGATCTCCATGGTCCA GGCCCGGGGTCGTGCTCGAGCTGGTCAGAGTGTGTACTCCTTCCTGGCTACAGAGGGCAGTCGGGAGATG AAGCGGGAGCTAACCAATGAGGCTCTGGAGGTGCTGATGGAGAAGGCTGTGGCTGCTGTACAGAAGATGG ACCCTGATGAGTTCAAGGCCAAGATCCGGGACTTGCAGCAAGCATCTCTCGTTAAGCGGGCAGCACGCGC GGCCCATCGGGAGATCCAGCAGGGGCAGTTCCTACCGGAGCACGTGCAACTTCTCTGCATCAACTGTATG GTGGCCGTGGGCTACGGGAGTGACCTGCGGAAAGTGGAGGGCACCCACCACGTCAATGTGAACCCCAACT TCTCGGTCTACTATACCACCTCCCAGAACCCTGTGGTCATTAACAAGGTCTTTAAGGACTGGAGACCTGG AGGAACCATCAGGTGCAGTAACTGTGGGGAGGTCTGGGGCTTCCAGATGATCTACAAATCAGTGACCTTG CCAGTGCTCAAAATCGGAAGCATGCTACTGGAAACACCTCGAGGGAAGATCCAGGCCAAAAAGTGGTCCC GGGTGCCTTTCTCCATACCAGTCTTCGATATCCTGCAGGACTGCACACAAAGCCTGTCTGAGCTCTCCCT GGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209973 protein sequence
Red=Cloning site Green=Tags(s) MELRPYQWEVILPALEGKNIIIWLPTGAGKTRAAAFVAKRHLETVDRGKVVVLVNRVHLVSQHAEEFRRM LDKHWTVTTLSGDMGSRAGFGLMARSHDLLICTAELLQLALNSSEEDEHVELREFSLIVVDECHHTHKDT VYNTILSRYLEQKLKKAEPLPQVLGLTASPGTGGATKLQGAIDHILQLCANLDTCHIMSPKNCYSQLLMH NPKPCKQYDLCQRRAQDPFGDLIKKLMNQIHQQLEMPDLKQQFGTQMYEQQVVQLCKDAAEAGLQEQRVY ALHLRRYNDALFIHDTVRARDALDMLQDFYDRERTTKTQMVRAESWLLKLFDDHKNVLGQLAARGPENPK LEMLERILLKQFGSPGHTRGIIFTRTRQTASSLLLWLRQQPCLQTVGIKPQMLIGAGNTSQSTHMTQKDQ QEVIQEFRDGILSLLVATSVAEEGLDIAQCNVVVRYGLLTNEISMVQARGRARAGQSVYSFLATEGSREM KRELTNEALEVLMEKAVAAVQKMDPDEFKAKIRDLQQASLVKRAARAAHREIQQGQFLPEHVQLLCINCM VAVGYGSDLRKVEGTHHVNVNPNFSVYYTTSQNPVVINKVFKDWRPGGTIRCSNCGEVWGFQMIYKSVTL PVLKIGSMLLETPRGKIQAKKWSRVPFSIPVFDILQDCTQSLSELSLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030150 |
| ORF Size | 2037 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030150.2, NP_084426.2 |
| RefSeq Size | 2427 bp |
| RefSeq ORF | 2037 bp |
| Locus ID | 80861 |
| UniProt ID | Q99J87 |
| MW | 76.7 kDa |
| Gene Summary | Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205827 | Dhx58 (untagged) - Mouse DEXH (Asp-Glu-X-His) box polypeptide 58 (Dhx58), (10ug) |
CNY 2,000.00 |
|
| MG209973 | Dhx58 (tGFP-tagged) - Mouse DNA segment, Chr 11, Lothar Hennighausen 2, expressed (D11Lgp2e) |
CNY 7,410.00 |
|
| MR209973L3 | Lenti ORF clone of Dhx58 (Myc-DDK-tagged) - Mouse DEXH (Asp-Glu-X-His) box polypeptide 58 (Dhx58) |
CNY 8,650.00 |
|
| MR209973L4 | Lenti ORF clone of Dhx58 (mGFP-tagged) - Mouse DEXH (Asp-Glu-X-His) box polypeptide 58 (Dhx58) |
CNY 8,650.00 |
