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Cnga3 (NM_009918) Mouse Tagged ORF Clone
CAT＃: MR209605
- TrueORF®
Cnga3 (Myc-DDK-tagged) - Mouse cyclic nucleotide gated channel alpha 3 (Cnga3)
ORF Plasmid: tGFP
"NM_009918" in other vectors (4)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CNG3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209605 representing NM_009918
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGGTGAACACCCAGTGTTCACAGCCCTCCCCGACCCAACTTTCAATAAAGAATGCGGACAGAG ATCTCGACCACGTAGAGAACGGCCTGGGCAGGGTGTCACGCCTCATCATCTCGATTCGTGCGTGGGCCTC CAGGCACTTACACGATGAAGACCAGACACCTGACTCCTTTTTGGATCGATTTCATGGATCTGAGCTTAAG GAAGTCTCCACCCGGGAAAGCAATGCCCAGCCCAACCCAGGAGAACAGAAGCCACCAGACGGAGGGGAAG GCAGGAAGGAGGAGCCCATTGTGGTGGACCCCTCCAGCAACATCTACTACCGCTGGCTGACTGCCATCGC GCTCCCAGTCTTCTATAACTGGTGTCTACTTGTATGCAGGGCCTGTTTTGATGAGCTACAATCAGAGCAC CTGACGCTGTGGCTGGTCTTGGACTACTCTGCAGATGTCCTTTATGTTTTAGACATGCTGGTTCGAGCCC GGACAGGTTTCCTTGAGCAAGGCCTAATGGTCAGAGATACCAAGAGGCTGTGGAAACATTACACAAAGAC CTTGCACTTCAAGCTGGACATCCTGTCTCTCATCCCCACAGACCTGGCTTATTTAAAGTTGGGCGTGAAC TACCCAGAACTGAGGTTCAACCGCCTCCTGAAGTTCTCTCGGCTCTTTGAATTCTTTGACCGCACAGAGA CAAGGACCAACTACCCCAACGTGTTCAGGATAGGGAACTTGGTCCTTTACACACTCATCATCATCCACTG GAATGCCTGCATCTACTTTGCCATTTCCAAGTTCATTGGTTTTGGGACAGACTCCTGGGTCTATCCAAAC ACCTCCAAGCCGGAGTATGCACGACTCTCCCGGAAGTACATTTACAGTCTCTACTGGTCCACCCTGACCC TGACCACCATTGGTGAGACCCCACCCCCCGTGAAAGATGAAGAGTATCTCTTTGTGGTCATCGACTTCCT GGTGGGTATCCTGATCTTTGCCACCATAGTGGGCAACGTAGGCTCCATGATTTCCAACATGAACGCTCCC CGGGTAGAGTTCCAGGCTAAGATCGACTCCGTCAAGCAGTACATGCAGTTCCGGAAGGTAACCAAGGACT TGGAGACTCGGGTTATCCGGTGGTTTGACTATCTGTGGGCCAACAGGAAGACGGTGGATGAAAAGGAAGT GCTCAAAAACCTCCCGGACAAGCTGAAGGCTGAGATCGCCATCAACGTGCACCTGGACACGCTGAAGAAG GTCCGAATCTTCCAGGACTGCGAGGCGGGGCTGCTGGTGGAGCTGGTGCTGAAGCTCCGCCCCACTGTGT TCAGCCCCGGGGACTACATATGCAAAAAGGGGGACATTGGAAGAGAGATGTACATCATCAAGGAGGGCAA GCTGGCTGTGGTGGCTGATGACGGGGTCACCCAGTTTGTGGTCCTCAGTGACGGCAGTTACTTTGGGGAG ATTAGCATCTTAAACATTAAGGGGAGCAAGTCTGGGAACCGCAGGACGGCCAACATCAGGAGTATCGGCT ACTCAGACCTGTTCTGCCTCTCCAAGGATGACCTAATGGAAGCCCTCACCGAGTACCCAGACGCTAAGAG GGCTCTGGAGGAAAAGGGCCGTCAGATTCTGATGAAGGACAACCTAATTGATGAGGACCTAGTCGCGGCC AGGGTAGATACCAGGGACGTTGAGGAGAAGGTGGAGTACCTGGAGTCGTCCCTGGACATCCTGCAGACGA GGTTTGCCCGACTCCTGGCTGAGTACAGTGCCTCCCAGATGAAGCTGAAACAGCGGCTCACTCGGCTGGA GAGCCAGATGAACAGGAGGTGTTGTGGCTTCTCACCTGACAGGGAGAATTCCGAGGATGCTTCAAAGACT GAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209605 representing NM_009918
Red=Cloning site Green=Tags(s) MAKVNTQCSQPSPTQLSIKNADRDLDHVENGLGRVSRLIISIRAWASRHLHDEDQTPDSFLDRFHGSELK EVSTRESNAQPNPGEQKPPDGGEGRKEEPIVVDPSSNIYYRWLTAIALPVFYNWCLLVCRACFDELQSEH LTLWLVLDYSADVLYVLDMLVRARTGFLEQGLMVRDTKRLWKHYTKTLHFKLDILSLIPTDLAYLKLGVN YPELRFNRLLKFSRLFEFFDRTETRTNYPNVFRIGNLVLYTLIIIHWNACIYFAISKFIGFGTDSWVYPN TSKPEYARLSRKYIYSLYWSTLTLTTIGETPPPVKDEEYLFVVIDFLVGILIFATIVGNVGSMISNMNAP RVEFQAKIDSVKQYMQFRKVTKDLETRVIRWFDYLWANRKTVDEKEVLKNLPDKLKAEIAINVHLDTLKK VRIFQDCEAGLLVELVLKLRPTVFSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGE ISILNIKGSKSGNRRTANIRSIGYSDLFCLSKDDLMEALTEYPDAKRALEEKGRQILMKDNLIDEDLVAA RVDTRDVEEKVEYLESSLDILQTRFARLLAEYSASQMKLKQRLTRLESQMNRRCCGFSPDRENSEDASKT D TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009918 |
| ORF Size | 1893 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009918.2 |
| RefSeq Size | 3512 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 12790 |
| UniProt ID | Q9JJZ8 |
| MW | 73.2 kDa |
| Gene Summary | Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones (By similarity). Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201900 | Cnga3 (untagged) - Mouse cyclic nucleotide gated channel alpha 3 (Cnga3), (10ug) |
CNY 2,000.00 |
|
| MG209605 | Cnga3 (tGFP-tagged) - Mouse cyclic nucleotide gated channel alpha 3 (Cnga3) |
CNY 5,040.00 |
|
| MR209605L3 | Lenti ORF clone of Cnga3 (Myc-DDK-tagged) - Mouse cyclic nucleotide gated channel alpha 3 (Cnga3) |
CNY 6,460.00 |
|
| MR209605L4 | Lenti ORF clone of Cnga3 (mGFP-tagged) - Mouse cyclic nucleotide gated channel alpha 3 (Cnga3) |
CNY 6,460.00 |
