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Man2a2 (BC055006) Mouse Tagged ORF Clone
CAT＃: MR208460
- TrueORF®
Man2a2 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha 2 (cDNA clone MGC:57099 IMAGE:6491507)
ORF Plasmid: tGFP
"BC055006" in other vectors (4)
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CNY 3,936.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700052O22Rik; 4931438M07Rik; AI480988; Man IIx; MX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208460 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACAGCCGTGTAAGCCATGATGCCCTGCCCGAGCGCACGGTGATCCGGCTGGACTCCTCACCCA GGTTTGTGGTGGTATTTAACCCGCTGGAACAGGAGCGGCTCAGTGTGGTGTCCCTGCTGGTCAACTCGCC CCGGGTGCGCGTCCTTTCAGAGGAGGGCCAGCCCCTGTCTGTGCAGATCAGTGTGCACTGGAGCTCGGCT ACGGACATGGTCCCTGATGTCTACCAGGTGTCAGTACCCGTCCGCCTGCCAGGCCTGGGCCTGGGTGTGC TGCAGCTGCAGCCAGATCTCGATGGGCCCTACACGCTGCAGTCTTCCGTGCGGGTCTACCTGAACGGCGT GAAACTGTCCGTCAGCAGGCAGTCAGCATTCCCTGTCCGTGTTGTGGACTCCGGCGCCAGCGACTTCGCC ATCAGCAATCGCTACATGCAGGTCTGGTTCTCCGGCCTTACTGGGCTTCTCAAGAGCATCCGACGGGTGG ATGAGGAGCAGGAGCAGCAGATGGAACTGGAGTTCCTCGTCTACGGCACCCGTACCTCCAAGGACAAGAG TGGCGCCTACCTCTTCCTGCCTGATAGCGAGGCTAAGCCCTACGTCCCTAAGAAACCTCCTGTGCTGCGT GTCACCGAAGGCCCTTTCTTCTCAGAGGTGGCTGTGTACTATGAGCACTTTCACCAAGTGATTCGACTTT ATAACCTGCCAGGGGTAGAGGGGCTGTCTCTGGACATGTCATTCCAGGTAGATATCAGGGACTATGTGAA CAAGGAACTGGCCCTGCGCATCCACACAGACATTGACAGCCAGGGCACTTTCTTCACAGACCTCAATGGC TTTCAGATACAGCCCCGGCAGTATCTGAAGAAGCTGCCCCTGCAGGCTAATTTCTACCCTATGCCAGTCA TGGCCTATATCCAAGATTCCCAGAGGCGCCTCACGCTGCACACTGCCCAGGCTCTGGGTGTCTCCAGCCT CGGTAATGGCCAGCTGGAGGTGATCTTGGACCGAAGGCTAATGCAGGATGACAACCGGGGACTAGGCCAA GGGCTCAAAGACAACAAGATCACCTGCAACCGTTTCCGCCTCCTGTTAGAACGTCGGACCACAATGAGCC CAGAGGTGCATCAGGAGCAGGAGCGTTCTACAAGCTACCCGTCCCTCCTCAGCCACCTGACATCCATGTA CCTCAGCACACCTCCTCTTGTCTTACCCGTGGCCAAGAGGCAGGGCACTAGCCCTGCTCTGCGCTCTTTC CACCCTCTGGCTTCTCCATTGCCCTGTGACTTCCATTTGCTCAATCTGCGCATGCTCCCAGCCGAGGACA CCTTGCCCGCAACTGACTCTGCGCTCATCTTACACCGCAAGGGGTTTGACTGTGGCCTTGAAGCCAAGAA CCTGGGCTTCAACTGTACCACAAGCCAAGGCAAGCTAGCCCTGGGGAGCCTCTTCCATGGCCTGGATGTG ACCTTCCTGCAGCCAACCTCCTTGACTTTGCTATACCCTCTGGCCTCACCTTCCAACAGCACTGACATCT CTCTGGAGCCCATGGAGATCAGCACCTTCCGCCTGCGCTTGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208460 protein sequence
Red=Cloning site Green=Tags(s) MDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVHWSSA TDMVPDVYQVSVPVRLPGLGLGVLQLQPDLDGPYTLQSSVRVYLNGVKLSVSRQSAFPVRVVDSGASDFA ISNRYMQVWFSGLTGLLKSIRRVDEEQEQQMELEFLVYGTRTSKDKSGAYLFLPDSEAKPYVPKKPPVLR VTEGPFFSEVAVYYEHFHQVIRLYNLPGVEGLSLDMSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNG FQIQPRQYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQ GLKDNKITCNRFRLLLERRTTMSPEVHQEQERSTSYPSLLSHLTSMYLSTPPLVLPVAKRQGTSPALRSF HPLASPLPCDFHLLNLRMLPAEDTLPATDSALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDV TFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC055006 |
| ORF Size | 1584 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055006, AAH55006 |
| RefSeq Size | 4659 bp |
| RefSeq ORF | 1586 bp |
| Locus ID | 140481 |
| MW | 59.2 kDa |
| Gene Summary | Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217956 | Man2a2 (untagged) - Mouse mannosidase 2, alpha 2 (cDNA clone MGC:57099 IMAGE:6491507), (10ug) |
CNY 6,940.00 |
|
| MG208460 | Man2a2 (tGFP-tagged) - Mouse mannosidase 2, alpha 2 (cDNA clone MGC:57099 IMAGE:6491507) |
CNY 3,330.00 |
|
| MR208460L3 | Lenti ORF clone of Man2a2 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha 2 (cDNA clone MGC:57099 IMAGE:6491507) |
CNY 4,940.00 |
|
| MR208460L4 | Lenti ORF clone of Man2a2 (mGFP-tagged) - Mouse mannosidase 2, alpha 2 (cDNA clone MGC:57099 IMAGE:6491507) |
CNY 4,940.00 |
