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Ap4m1 (NM_021392) Mouse Tagged ORF Clone
CAT＃: MR207171
- TrueORF®
Ap4m1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-4, mu 1 (Ap4m1)
ORF Plasmid: tGFP
"NM_021392" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930443L05Rik; Ap4m4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207171 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTTCCCAGTTCTTCATTCTGTCTTCCAAGGGGGATCCGCTCATCTATAAAGACTTCCGCGGGGACA GTGGTGGTCGTGATGTGGCAGAGCTCTTCTACCGGAAGCTGACGGGACTGCCTGGAGGCGAGTCCCCGGT TGTCATGTATCACGGTGATCGTCATTTTATTCACATCAGACACAGTGGGCTCTATTTGGTGGCCACAACC TTAGAAAACGTCTCTCCTTTCAGCCTTCTGGAGCTGCTTTCCCGGCTAGCCACTCTCTTGGGTGACTACT GTGGCTCACTCAATGAGGGAACCATCTCACGCAATGTGGCGCTTGTCTACGAACTCCTGGATGAAGTGCT GGATTATGGCTATGTGCAGACTACATCCACAGAAATGCTGAGGAACTTCATCCAAACTGAAGCTGTGGTC AGCAAGCCCTTCAGCCTCTTTGACCTCAGCAGTGTTGGATTGTTCGGGGCAGAGACACAGCAGAATAAAG TGGCCCCAAGCAGCGCAGCCAGCCGCCCTGTCCTGTCCAGTCGTTCTGACCAGAGCCAAAAGAATGAGGT GTTTTTGGATGTGGTGGAGAGACTGTCTGTACTGATTGCATCTAATGGCTCGTTGTTGAAGGTGGACGTC CAAGGAGAGATACGGCTCAAGAGCTTCCTTCCCAGCGGTTCTGAGATATGCATTGGCTTGACAGAAGAAT TTTGTGTTGGAAAGTCAGAACTGAGAGGTTATGGGCCAGGGATTCGAGTTGATGAGGTGTCATTCCATAG TTCTGTCAATCTAGATGAGTTTGAGTCTCATCGGATCCTCCGCCTGCAGCCACCTCAGGGCGAGCTGACT GTGATGAGATACCAGCTCTCTGATGACCTCCCCTCACCACTCCCCTTCCGGCTCTTTCCCTCTGTGCAGT GGGACCAAGGCTCAGGCCGGCTCCAGGTTTACCTGAAGTTACGGTGTGACCTGCCCCCAAAGAGCCAAGC TCTCAACATTCATCTGCACCTTCCCCTGCCCCGAGGGGTCATCAGCCTGTCTCAGGAACTGAGCAGTCCA GATCAGAAGGCAGAGCTGGGAGAAGGAGCCCTTCACTGGGATCTGCCCCGGGTACAAGGAGGCTCTCAAC TCTCCGGCCTTTTCCAGATGGATGTCCCTGGCTTGCAGGGACTTCCCAACCATGGACCTTCTCCCTTGGG GCTGGGTCCTGCCAGCCTCTCCTTTGAACTCCCTCGGCACACATGCTCCGGTCTCCAGGTTCGATTCCTC AGACTGTCCTTTAGTGCCTGTGGTAATGCCAATCCTCACAAGTGGGTTCGACATCTAAGCCACAGCAACG CCTACGTAATTCGGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207171 protein sequence
Red=Cloning site Green=Tags(s) MISQFFILSSKGDPLIYKDFRGDSGGRDVAELFYRKLTGLPGGESPVVMYHGDRHFIHIRHSGLYLVATT LENVSPFSLLELLSRLATLLGDYCGSLNEGTISRNVALVYELLDEVLDYGYVQTTSTEMLRNFIQTEAVV SKPFSLFDLSSVGLFGAETQQNKVAPSSAASRPVLSSRSDQSQKNEVFLDVVERLSVLIASNGSLLKVDV QGEIRLKSFLPSGSEICIGLTEEFCVGKSELRGYGPGIRVDEVSFHSSVNLDEFESHRILRLQPPQGELT VMRYQLSDDLPSPLPFRLFPSVQWDQGSGRLQVYLKLRCDLPPKSQALNIHLHLPLPRGVISLSQELSSP DQKAELGEGALHWDLPRVQGGSQLSGLFQMDVPGLQGLPNHGPSPLGLGPASLSFELPRHTCSGLQVRFL RLSFSACGNANPHKWVRHLSHSNAYVIRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021392 |
| ORF Size | 1350 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021392.4 |
| RefSeq Size | 1734 bp |
| RefSeq ORF | 1350 bp |
| Locus ID | 11781 |
| UniProt ID | Q9JKC7 |
| MW | 49.5 kDa |
| Gene Summary | Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system (By similarity). It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons (PubMed:18341993). Within AP-4, the mu-type subunit AP4M1 is directly involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos. The adaptor protein complex 4 (AP-4) may also recognize other types of sorting signal (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201133 | Ap4m1 (untagged) - Mouse adaptor-related protein complex AP-4, mu 1 (Ap4m1), (10ug) |
CNY 2,000.00 |
|
| MG207171 | Ap4m1 (tGFP-tagged) - Mouse adaptor-related protein complex AP-4, mu 1 (Ap4m1) |
CNY 3,710.00 |
|
| MR207171L3 | Lenti ORF clone of Ap4m1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-4, mu 1 (Ap4m1) |
CNY 5,230.00 |
|
| MR207171L4 | Lenti ORF clone of Ap4m1 (mGFP-tagged) - Mouse adaptor-related protein complex AP-4, mu 1 (Ap4m1) |
CNY 5,230.00 |
