United States
Germany
Japan
United Kingdom
China
Gcm1 (NM_008103) Mouse Tagged ORF Clone
CAT＃: MR206959
- TrueORF®
Gcm1 (Myc-DDK-tagged) - Mouse glial cells missing homolog 1 (Drosophila) (Gcm1)
"NM_008103" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gcm-rs2; Gcm1-rs1; GCMa; glide |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206959 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTGGACGACTTTGATCCTGAAGACAAAGAGATACTGAGCTGGGACATTAACGATGTGAAACTGC CTCAGAACGTGAAAACGACTGACTGGTTCCAGGAGTGGCCGGACTCCTACGTGAAACACATCTACAGCTC GGACGACAGGAACGCACAGCGCCACCTGAGCAGCTGGGCCATGCGCAACACCAACAACCACAACTCCCGG ATCCTCAAGAAGTCATGCCTGGGGGTAGTGGTGTGCAGCAGGGACTGCTCCACAGAGGAAGGCCGCAAGA TTTACCTGAGACCCGCCATCTGTGACAAAGCCAGACAGAAGCAGCAGAGGAAAAGCTGTCCCAACTGCAA TGGACCCCTGAAGCTTATTCCCTGCCGAGGCCACGGCGGCTTCCCGGTCACCAACTTCTGGAGGCACGAC GGACGCTTCATCTTTTTCCAGTCCAAAGGCGAGCATGATCATCCCAGGCCGGAGACCAAGCTGGAAGCAG AGGCAAGAAGAGCCATGAAGAAAGTGCACATGGCATCTGCCTCCAACTCCTTACGGATGAAGGGGAGACC AGCAGCGAAGGCGCTTCCTGCTGAAATCCCGAGTCAGGGAAGTTTACCTTTAACTTGGTCATTCCAGGAA GGCGTCCAACTGCCCGGCACTTACAGCACACCTTTAATAGCTAACGCCCCCCAGCAGAACTCCCTGAATG ATTGCTTATCCTTCCCCAAGAGTTATGATTTGGGGGGAAGCACTGAGCTGGAAGATCCAACTTCCACCTT AGATTCCATGAAGTTCTATGAGAGATGCAAATTCTCCAGCAGTAGGATCTACGGCAGTGAAGAGCAGTTT CAGCCTCCTGTCCCTGGGACGTATGGAGACTACGAAGACCTGCAAACTTGGAATAAAAATGTTGCCTTAG GGAGAAATCCCTCCGATGACATCTACTATCCAGCCTATCCTCTGCCTGTGGCCAGCTGGCCCTACGACTA CTTTCCCTCCCAGAACTCTTTGGAGCACTTACCCCAGCAAGTTCCATCAGAACCCCCTGCAGCTCAACCA GGCTGTCATCCCTTGTGGTCCAATCCGGGAGGTGAGCCTTACGAAGAGAAAGTATCTGTGGATCTGAGCA GCTATGTGCCCTCCCTCACGTACCACCCACCTCAGCAGGATCCCTTCCTGCTCACCTATGGCTCTCCTAC TCAGCAGCAACATGCACTGCCCGGCAAGAGCAACAGGTGGGATTTTGACGAAGAGATGGCATGCATGGGC TTAGATCACTTCAACAATGAGATGCTCCTAAACTTCTGTTCTTTAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206959 protein sequence
Red=Cloning site Green=Tags(s) MELDDFDPEDKEILSWDINDVKLPQNVKTTDWFQEWPDSYVKHIYSSDDRNAQRHLSSWAMRNTNNHNSR ILKKSCLGVVVCSRDCSTEEGRKIYLRPAICDKARQKQQRKSCPNCNGPLKLIPCRGHGGFPVTNFWRHD GRFIFFQSKGEHDHPRPETKLEAEARRAMKKVHMASASNSLRMKGRPAAKALPAEIPSQGSLPLTWSFQE GVQLPGTYSTPLIANAPQQNSLNDCLSFPKSYDLGGSTELEDPTSTLDSMKFYERCKFSSSRIYGSEEQF QPPVPGTYGDYEDLQTWNKNVALGRNPSDDIYYPAYPLPVASWPYDYFPSQNSLEHLPQQVPSEPPAAQP GCHPLWSNPGGEPYEEKVSVDLSSYVPSLTYHPPQQDPFLLTYGSPTQQQHALPGKSNRWDFDEEMACMG LDHFNNEMLLNFCSLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008103 |
| ORF Size | 1311 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008103.3, NP_032129.2 |
| RefSeq Size | 2034 bp |
| RefSeq ORF | 1311 bp |
| Locus ID | 14531 |
| UniProt ID | P70348 |
| MW | 49.6 kDa |
| Gene Summary | Transcription factor that is necessary for placental development (PubMed:8962155). Involved in the control of expression of placental growth factor (PGF) and other placenta-specific genes. Binds to the trophoblast-specific element 2 (TSE2) of the aromatase gene enhancer. Binds to the SYDE1 promoter. Has a central role in mediating the differentiation of trophoblast cells along both the villous and extravillous pathways in placental development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215698 | Gcm1 (untagged) - Mouse glial cells missing homolog 1 (Drosophila) (Gcm1), (10ug) |
CNY 5,488.00 |
|
| MR206959L3 | Lenti ORF clone of Gcm1 (Myc-DDK-tagged) - Mouse glial cells missing homolog 1 (Drosophila) (Gcm1) |
CNY 6,270.00 |
|
| MR206959L4 | Lenti ORF clone of Gcm1 (mGFP-tagged) - Mouse glial cells missing homolog 1 (Drosophila) (Gcm1) |
CNY 6,270.00 |
