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Pofut1 (NM_080463) Mouse Tagged ORF Clone
CAT＃: MR206140
- TrueORF®
Pofut1 (Myc-DDK-tagged) - Mouse protein O-fucosyltransferase 1 (Pofut1)
ORF Plasmid: tGFP
"NM_080463" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mKIAA0180; O-FucT-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206140 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCCGCCGCGTGGGCACCGCCACACCTGCTGCTGCGGGCGTCTTTCCTGCTTCTGCTGCTGTTGC TGCCGCTCCGCGGGCGGTCAGCGGGCTCCTGGGACCTGGCCGGTTACCTGCTCTACTGTCCCTGCATGGG GCGCTTTGGGAACCAGGCTGATCACTTCTTGGGCTCCCTGGCATTTGCGAAGCTGCTGAACCGCACCTTG GCTGTACCTCCATGGATTGAATACCAACATCACAAGCCTCCTTTCACCAACCTCCATGTGTCCTACCAAA AGTACTTCAAACTGGAGCCTCTCCAAGCCTACCATCGGGTTGTCAGCCTGGAGGACTTCATGGAAAATCT GGCACCCTCCCACTGGCCCCCTGAGAAGCGAGTGGCATACTGCTTTGAGGTGGCAGCCCAGCGAAGTCCT GATAAGAAGACATGTCCCATGAAGGAAGGAAATCCTTTTGGGCCATTCTGGGACCAGTTTCATGTGAGTT TCAATAAGTCAGAACTGTTCACAGGCATTTCCTTCAGCGCCTCCTACAAAGAACAATGGACCCAGAGATT TCCTGCAAAAGAGCATCCTGTGCTCGCACTGCCTGGGGCCCCAGCACAGTTCCCTGTCCTGGAGGAACAC AGGGAGCTCCAGAAGTACATGGTGTGGTCAGATGAGATGGTGAGGACGGGAGAGGCCCTGATCAGTGCCC ACCTCGTCCGGCCCTATGTGGGCATTCATCTGCGCATTGGCTCCGACTGGAAGAATGCCTGTGCCATGCT GAAGGATGGAACTGCAGGGTCACACTTCATGGCTTCCCCTCAGTGTGTGGGCTATAGCCGCAGCACAGCG ACCCCTCTCACCATGACCATGTGCCTCCCTGACCTGAAGGAAATCCAGCGGGCTGTGACGCTTTGGGTGA GAGCACTGAATGCCAGATCGGTCTACATCGCCACAGACTCTGAGAGCTACGTGTCAGAGATCCAGCAGCT CTTCAAAGACAAGGTGAGGGTGGTGAGCCTGAAACCCGAGGTGGCCCAGATCGACCTGTACATCCTCGGC CAGGCTGACCACTTCATTGGAAACTGTGTCTCCTCGTTCACTGCCTTCGTGAAGCGGGAGCGGGACCTCC ATGGGAGGCAGTCGTCCTTCTTTGGCATGGACAGACCCTCCCAGCTTCGGGATGAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206140 protein sequence
Red=Cloning site Green=Tags(s) MGAAAWAPPHLLLRASFLLLLLLLPLRGRSAGSWDLAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTL AVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVVSLEDFMENLAPSHWPPEKRVAYCFEVAAQRSP DKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYKEQWTQRFPAKEHPVLALPGAPAQFPVLEEH RELQKYMVWSDEMVRTGEALISAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTA TPLTMTMCLPDLKEIQRAVTLWVRALNARSVYIATDSESYVSEIQQLFKDKVRVVSLKPEVAQIDLYILG QADHFIGNCVSSFTAFVKRERDLHGRQSSFFGMDRPSQLRDEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080463 |
| ORF Size | 1182 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080463.3, NP_536711.3 |
| RefSeq Size | 5618 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 140484 |
| UniProt ID | Q91ZW2 |
| MW | 44.7 kDa |
| Gene Summary | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203080 | Pofut1 (untagged) - Mouse protein O-fucosyltransferase 1 (Pofut1), (10ug) |
CNY 2,000.00 |
|
| MG206140 | Pofut1 (tGFP-tagged) - Mouse protein O-fucosyltransferase 1 (Pofut1), transcript variant 1 |
CNY 3,140.00 |
|
| MR206140L3 | Lenti ORF clone of Pofut1 (Myc-DDK-tagged) - Mouse protein O-fucosyltransferase 1 (Pofut1) |
CNY 4,750.00 |
|
| MR206140L4 | Lenti ORF clone of Pofut1 (mGFP-tagged) - Mouse protein O-fucosyltransferase 1 (Pofut1) |
CNY 4,750.00 |
