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Pcgf6 (NM_027654) Mouse Tagged ORF Clone
CAT＃: MR205368
- TrueORF®
Pcgf6 (Myc-DDK-tagged) - Mouse polycomb group ring finger 6 (Pcgf6)
ORF Plasmid: tGFP
"NM_027654" in other vectors (3)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4933407A11Rik; AI604840; MBLR; Rnf134 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205368 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGGCTGAGACGGACGCCACGGAAAACAAGCGCGCCTCTGAGGCCAAGCGAGCCTCAGCCATGC CGCCACCGCCACCGCCTCCGCCTCCCATCTCTCCGCCCGCCCTCATCCCGGCACCCGCGGCCGGCGAGGA GGGCCCGGCGTCCCTGGGCCAGGCGGGCGCTGCCGGCTGCTCTCGCTCTCGGCCCCCGGCGTTGGAGCCC GAGCGCAGCCTGGGCCGCTTGAGAGGCCGCTTCGAGGACTACGATGAAGAGTTGGAAGAGGAGGAGGAGA TGGAGGAGGAAGAAGAGGAGGAGGAAGAGATGAGCCACTTCTCTCTGCGTCTGGAGTCCGGACGGGCAGA CTCCGAGGACGAGGAGGAGCGCCTGATAAACCTTGTCGAGCTGACCCCCTACATCCTGTGTTCGATTTGC AAAGGTTACCTGATAGATGCAACCACCATTACAGAGTGTCTTCATACCTTTTGTAAAAGCTGCATTGTGA GACATTTTTACTATAGTAACAGATGCCCAAAATGCAACATAGTTGTCCACCAGACTCAGCCTCTTTATAA TATAAGGTTGGACCGGCAGTTACAAGACATAGTATACAAATTAGTGATCAATCTAGAGGAAAGAGAAAAA AAGCAAATGCATGACTTCTATAAAGAAAGAGGTCTAGAAGTCCCCAAGCCTGCTGCTCCACAGCCAGTCC CTTCAAGCAAAGGAAAAACCAAGAAGGTCCTAGAGTCAGTGTTTCGTATTCCACCTGAACTTGATATGTC TTTATTACTTGAGTTCATTGGTGCTAATGAAGACACCGGACATTTTAAGCCATTGGAAAAGAAGTTTGTG CGTGTTTCAGGAGAAGCAACTATCGGGCATGTAGAAAAATTTCTTAGAAGAAAAATGGGTCTTGATCCAG CTTGTCAGGTAGATATCATCTGTGGGGATCACTTACTGGAGCGGTACCAAACTCTACGGGAAATCCGACG TGCAATAGGTGATACAGCAATGCAGGACGGTCTGCTTGTTCTTCATTATGGTCTTGTGGTTTCTCCTTTG AAAATAACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205368 protein sequence
Red=Cloning site Green=Tags(s) MDEAETDATENKRASEAKRASAMPPPPPPPPPISPPALIPAPAAGEEGPASLGQAGAAGCSRSRPPALEP ERSLGRLRGRFEDYDEELEEEEEMEEEEEEEEEMSHFSLRLESGRADSEDEEERLINLVELTPYILCSIC KGYLIDATTITECLHTFCKSCIVRHFYYSNRCPKCNIVVHQTQPLYNIRLDRQLQDIVYKLVINLEEREK KQMHDFYKERGLEVPKPAAPQPVPSSKGKTKKVLESVFRIPPELDMSLLLEFIGANEDTGHFKPLEKKFV RVSGEATIGHVEKFLRRKMGLDPACQVDIICGDHLLERYQTLREIRRAIGDTAMQDGLLVLHYGLVVSPL KIT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027654 |
| ORF Size | 1062 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027654.3 |
| RefSeq Size | 2126 bp |
| RefSeq ORF | 1062 bp |
| Locus ID | 71041 |
| UniProt ID | Q99NA9 |
| MW | 39.8 kDa |
| Gene Summary | Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205368 | Pcgf6 (tGFP-tagged) - Mouse polycomb group ring finger 6 (Pcgf6) |
CNY 3,710.00 |
|
| MR205368L3 | Lenti ORF clone of Pcgf6 (Myc-DDK-tagged) - Mouse polycomb group ring finger 6 (Pcgf6) |
CNY 5,230.00 |
|
| MR205368L4 | Lenti ORF clone of Pcgf6 (mGFP-tagged) - Mouse polycomb group ring finger 6 (Pcgf6) |
CNY 5,230.00 |
