United States
Germany
Japan
United Kingdom
China
Mrm3 (NM_183263) Mouse Tagged ORF Clone
CAT＃: MR204412
- TrueORF®
Rnmtl1 (Myc-DDK-tagged) - Mouse RNA methyltransferase like 1 (Rnmtl1)
ORF Plasmid: tGFP
"NM_183263" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,810.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4833420N02Rik; AI255153; HC90; Rnmtl1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204412 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAATAGTTAAGTCCAGGCCTTTTCGGGAAAAGCAAGGGAAGATCCTACTGGAAGGTCGCAGGCTGA TTGCAGATGCTCTCAAGGCTGGAGCTGTGCCCAAAGCGTTCTTTTTTAGCCGTCTGGAGTACGTCAAGGA GTTGCCGGTAGACAAGCTGAAAGACGTCAGCCTCATTAAGGTGAAATTTGAGGACATCAAGGATTGGTCA GACCTAGTAACGCCACAAGGAATAATGGGGATTTTTGCCAAACCTGACCCTGTTAAGATGACATATCCAG AGACTCCGCTTCACCATACACTGCCCCTAGTGTTGATTTGTGACAATCTCCGTGACCCTGGGAACCTGGG GACAATCCTGAGATCTGCAGCTGGAGCAGGCTGCAGTAAAGTCTTACTCACCAAAGGCTGTGTGGATGCC TGGGAGCCTAAAGTGCTCCGGGCAGGCATGGGGGCACATTTCCAGGTACCCATTGTGAACAATGTGGAAT GGGAAACAGTGCCCAATCACTTGCCTCCTGACACTCGAGTCTATGTGGCAGACAACTGTGGTCACTATGC CCAGGTTCAGATGTCTGATAAAACCGGTGACCGTGACTGGGCATGTGATCGCCGATTCTTAAAGTTTCAC AAGTATGAAGAGGACCTAGACACTAAAACCAGAAAGGACTGGCTGCCCAAACTTGAGGTCCAGAGTTATG ATTTGGACTGGACAGGAGCACCAGCAGCTGTGGTGATAGGTGGGGAGACGCATGGAGTGAGCCTGGAATC CCTGCAACTGGCCGAGAGTACCGGTGGCAAGAGACTGCTGATCCCCGTTGTACCTGGTGTGGACAGTCTG AACTCAGCCATGGCTGCGAGCATCCTGCTCTTTGAAGGGAAAAGGCAGCTGAGGATAAAGGTGGAAGACT TGAGCAGGGACAGGAGTTACCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204412 protein sequence
Red=Cloning site Green=Tags(s) MTIVKSRPFREKQGKILLEGRRLIADALKAGAVPKAFFFSRLEYVKELPVDKLKDVSLIKVKFEDIKDWS DLVTPQGIMGIFAKPDPVKMTYPETPLHHTLPLVLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDA WEPKVLRAGMGAHFQVPIVNNVEWETVPNHLPPDTRVYVADNCGHYAQVQMSDKTGDRDWACDRRFLKFH KYEEDLDTKTRKDWLPKLEVQSYDLDWTGAPAAVVIGGETHGVSLESLQLAESTGGKRLLIPVVPGVDSL NSAMAASILLFEGKRQLRIKVEDLSRDRSYH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183263 |
| ORF Size | 936 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183263.5, NP_899086.2 |
| RefSeq Size | 1492 bp |
| RefSeq ORF | 1257 bp |
| Locus ID | 67390 |
| UniProt ID | Q5ND52 |
| MW | 34.7 kDa |
| Gene Summary | S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1370 (Gm1370) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a conserved modification in the peptidyl transferase domain of the mtLSU rRNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210545 | Rnmtl1 (untagged) - Mouse RNA methyltransferase like 1 (Rnmtl1), (10ug) |
CNY 3,990.00 |
|
| MG204412 | Rnmtl1 (tGFP-tagged) - Mouse RNA methyltransferase like 1 (Rnmtl1) |
CNY 2,850.00 |
|
| MR204412L3 | Lenti ORF clone of Rnmtl1 (Myc-DDK-tagged) - Mouse RNA methyltransferase like 1 (Rnmtl1) |
CNY 4,750.00 |
|
| MR204412L4 | Lenti ORF clone of Rnmtl1 (mGFP-tagged) - Mouse RNA methyltransferase like 1 (Rnmtl1) |
CNY 4,750.00 |
