Cenpo (NM_134046) Mouse Tagged ORF Clone
CAT#: MR204145
- TrueORF®
Cenpo (Myc-DDK-tagged) - Mouse centromere protein O (Cenpo)
ORF Plasmid: tGFP
"NM_134046" in other vectors (4)
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CNY 2,081.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2810429O05Rik; 8430427C03Rik; D12Ertd482e |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204145 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTGCAAATACTTTGTGTCCAGGCAGGAAGTGCAAAGGAGGTGTTTTAGCTCACCTGGAAAGAC TAGAGGCTCAAACGAACGTATCGAATAGAAAGTCTGAAGAACCAACAGTTCGTAAAAAGGAAAGTTCTCT TAGAACCAAGATTCGCGAACTGCGGCAACAACGAGATAAGCTGAGGGCAGAAGTGAAGCAGTGGGGAGCC AGAGTTAAAGAACCTCCTGCCAAAGAAGACCCAAGCAGAACCGTGATCAGTGAACAGGAAGTTCTGGAAA GGGAGTGGAGAAATGTGGACGCCATTCTGGAGGCATATCGCTTTACGGGGCTCAGCGGGAAGTTGACCAG TCGTGGCGTCTGCATGTGCATCAGCACAGCCTTTGAGGGGAACCTACTAGATTCCTACTTTGTGGACCTT GTCATAGAGAAACCACTCCGGATTCATCACCATTCCGTCCCAGTCTTCATCCCTCTGGAAAAGATAGCCG CAGCACACCTACAGACTGATGTTCAGCGCTTCCTGTTTAGTCTCTGGGCATACCTAAATGCCTACGCGGG GAGGAAGTACCAGGCAGACCAACTTGAGAGTGACTTCTGCGATGTCCTCACGGGACCTTTGCAGAGAAAC GCCCTGTGCAACTTGCTGTCGTTCACCTATAAAGTGGAACAGAGATGCCAGACCTTCTCCTTCAGTGCCA GGTTGCTGTATGAGGACCCCACAGCAGCTCTTCCCACGAATGTCACTGTGACAAGGCCAGGTGTGGAGGC ATCATCCCCTCCTTGGGAAGAGCACAGAGCATCCCATCAGATGCTCTTCCGTACCAAGCCCCTGCACAAG GTGTTTGCTTCATTTTCCAAAGAAACCGAAAAGCTGCATTTGAACCTGGTCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204145 protein sequence
Red=Cloning site Green=Tags(s) MESANTLCPGRKCKGGVLAHLERLEAQTNVSNRKSEEPTVRKKESSLRTKIRELRQQRDKLRAEVKQWGA RVKEPPAKEDPSRTVISEQEVLEREWRNVDAILEAYRFTGLSGKLTSRGVCMCISTAFEGNLLDSYFVDL VIEKPLRIHHHSVPVFIPLEKIAAAHLQTDVQRFLFSLWAYLNAYAGRKYQADQLESDFCDVLTGPLQRN ALCNLLSFTYKVEQRCQTFSFSARLLYEDPTAALPTNVTVTRPGVEASSPPWEEHRASHQMLFRTKPLHK VFASFSKETEKLHLNLVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_134046 |
ORF Size | 897 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134046.1 |
RefSeq Size | 4343 bp |
RefSeq ORF | 897 bp |
Locus ID | 52504 |
UniProt ID | Q8K015 |
MW | 34 kDa |
Gene Summary | Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201628 | Cenpo (untagged) - Mouse centromere protein O (Cenpo), (10ug) |
CNY 2,000.00 |
|
MG204145 | Cenpo (tGFP-tagged) - Mouse centromere protein O (Cenpo) |
CNY 2,850.00 |
|
MR204145L3 | Lenti ORF clone of Cenpo (Myc-DDK-tagged) - Mouse centromere protein O (Cenpo) |
CNY 4,750.00 |
|
MR204145L4 | Lenti ORF clone of Cenpo (mGFP-tagged) - Mouse centromere protein O (Cenpo) |
CNY 4,750.00 |