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Kcne3 (NM_001190950) Mouse Tagged ORF Clone
CAT＃: MR200339
- TrueORF®
Kcne3 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, Isk-related subfamily, gene 3 (Kcne3), transcript variant 5
"NM_001190950" in other vectors (2)
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CNY 1,200.00
CNY 2,945.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2210017H05Rik; MiRP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR200339 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTTCCAACGGGACTGAGACCTGGTACATGAGCCTCCATGCTGTGCTGAAGGCTCTGAACACAA CCCTTCACAGTCACTTGCTCTGCCGGCCTGGGCCAGGACCAGGGCCAGACAATCAAACTGAGGATCGTCG GGCTAGCCTTCCTGGTCGTAATGACAACTCCTACATGTATATTCTCTTTGTCATGTTCCTATTTGCCGTC ACTGTGGGCAGTCTCATCCTGGGATATACCCGTTCACGCAAAGTGGACAAACGTAGTGACCCCTATCATG TGTACATCAAGAACCGTGTGTCTATGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200339 protein sequence
Red=Cloning site Green=Tags(s) METSNGTETWYMSLHAVLKALNTTLHSHLLCRPGPGPGPDNQTEDRRASLPGRNDNSYMYILFVMFLFAV TVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190950 |
| ORF Size | 312 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190950.1, NP_001177879.1 |
| RefSeq Size | 816 bp |
| RefSeq ORF | 312 bp |
| Locus ID | 57442 |
| UniProt ID | Q9WTW2 |
| MW | 11.7 kDa |
| Gene Summary | Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Associated with KCNC4/Kv3.4 is proposed to form the subthreshold voltage-gated potassium channel in skeletal muscle and to establish the resting membrane potential (RMP) in muscle cells. Associated with KCNQ1/KCLQT1 may form the intestinal cAMP-stimulated potassium channel involved in chloride secretion that produces a current with nearly instantaneous activation with a linear current-voltage relationship.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR200339L3 | Lenti ORF clone of Kcne3 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, Isk-related subfamily, gene 3 (Kcne3), transcript variant 5 |
CNY 4,750.00 |
|
| MR200339L4 | Lenti ORF clone of Kcne3 (mGFP-tagged) - Mouse potassium voltage-gated channel, Isk-related subfamily, gene 3 (Kcne3), transcript variant 5 |
CNY 4,750.00 |
