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Iqsec1 (NM_001134384) Mouse Tagged ORF Clone
CAT＃: MG227689
- TrueORF®
Iqsec1 (tGFP-tagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001134384" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW561907; BC026481; BRAG2; cI-43; D6Ertd349e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227689 representing NM_001134384
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGCCGAAGACGCTATTTTGTTGAGGGCGAGGCCCCCAGCAGCGAGACTGGCACATCGCTGGACA GCCCCTCAGCCTACCATCAGGGTCCCCTGGTACCTGGTTCCAGCCTGAGTCCAGACCACTACGAGCACAC ATCAGTGGGCGCCTATGGGCTGTACGCAGGGCCAGGACCACAACAGCGCACACGGAGGCCCCGGCTGCAG CACTCGACCTCAGTCCTGCGCAAGCAGGCTGAGGAGGAGGCCATCAAGCGCTCTCGGTCACTCTCTGAGA GCTATGAGCTCTCTTCAGATCTGCAAGACAAGCAGGTGGAAATGCTAGAACGCAAGTATGGGGGACGCCT CGTGACCCGCCATGCAGCCCGCACCATCCAGACGGCGTTCCGCCAATACCAGATGAACAAGAACTTTGAG CGCCTGCGCAGCTCCATGTCGGAGAACCGCATGTCACGTCGCATTGTGCTGTCCAACATGAGGATGCAGT TCTCCTTTGAGGGCCCCGAGAAGGTGCACAGCTCCTACTTTGAGGGAAAGCAGGTCTCTGTGACTAATGA TGGCTCCCAGCTGGGGGCCCTGGTGCCATCAGAATGTGGAGACCTTAGTGACCCAGCCCTCAAGTCTCCA GCCCCCTCCAGTGACTTTGCAGATGCCATCACGGAACTGGAGGATGCCTTCTCCAGGCAGGTGAAATCCC TGGCTGAATCCATCGATGATGCCCTCAATTGCCGCAGCCTACACAGTGAGGAGGTACCAGCCTCAGACAC GGCCCGGGCTCGGGACACTGAGCCCAAACCAGGTCTACATGGCATGGACCACCGCAAACTGGATGAGATG ACAGCCTCGTATAGTGATGTGACCCTCTACATTGATGAGGAAGAGCTGTCACCACCGCTTCCACTCTCAC AGGCTGGCGATCGGCCCTCCAGCACAGAGTCGGACCTGCGGCTGAGGTCTGGGGGTGCAGCCCAGGACTA CTGGGCCTTGGCCCACAAAGAGGACAAGGCTGACACGGACACAAGCTGCCGAAGCACACCATCACTGGAG CGGCCAGAGCCACGGCTGCGGGTGGAGCACCTGCCCCTGCTTACCATTGAGCCACCCAGTGATAGCTCCG TGGAACTCAGTGACCGCTCAGATCGCAGCTCACTCAAGAGGCAGAGTGCCTATGAGCGCAGCCTCGGTGG GCAGCAGGGCAGCCCCAAGCATGGCCCCCACGGTGGCCCCCCCAAAGGCCTCCCCAGGGAGGAACCTGAA TTGCGGCCTCGGCCCCCCAGACCTCTTGAGAGTCACCTAGCCATCAATGGTTCAGCCAACCGGCAGAGCA AATCTGAGTCTGACTACTCAGATGGGGACAATGACAGCATCAACAGCACCTCCAACTCCAATGACACCAT AAACTGCAGCTCTGAGTCCTCATCACGGGACAGCCTGCGGGAACAGACACTCAGCAAGCAGACATACCAC AAGGAGACCCGCAACAGCTGGGACTCTCCAGCTTTCAGCAACGATGTCATCCGTAAGAGGCATTACCGAA TTGGCCTAAATCTCTTCAACAAGAAGCCTGAGAAGGGCATCCAGTATCTCATTGAGCGCGGCTTCGTGCC CGACACGCCAGTGGGAGTGGCCCACTTCCTGCTGCAGCGCAAGGGCCTCAGCCGCCAGATGATCGGTGAG TTCCTGGGAAACCGGCAGAAGCAGTTCAACCGTGATGTGCTCGACTGTGTTGTGGACGAGATGGACTTCT CTGCCATGGAGCTAGATGAAGCCCTCCGGAAGTTCCAGGCACACATCCGAGTCCAAGGGGAGGCTCAGAA AGTGGAGCGGCTCATCGAGGCATTCAGCCAGCGGTACTGTGTCTGCAACCCAGGGGTGGTGCGACAGTTC CGGAACCCAGACACCATCTTCATCCTGGCCTTCGCCATCATCCTGCTCAACACAGACATGTACAGCCCCA ATGTCAAGCCAGAGCGCAAAATGAAGCTGGAGGACTTTGTCAAGAACCTCCGAGGTGTGGATGACGGTGA AGACATTCCCCGGGAGACGCTAATTGGGATCTATGAGCGAATCCGTAAGCGAGAACTGAAGACCAACGAG GACCACGTGTCCCAGGTGCAGAAGGTTGAGAAGCTCATCGTGGGCAAGAAGCCGATTGGATCCCTGCATC ATGGTCTTGGCTGTGTGCTGTCTCTTCCCCATCGACGACTAGTCTGCTACTGCCGGCTTTTTGAAGTTCC TGACCCCAACAAACCCCAGAAGCTGGGACTACACCAGCGAGAGATCTTCCTGTTTAATGACCTCCTGGTG GTCACCAAGATCTTCCAGAAGAAGAAGAACTCGGTGACATACAGCTTCCGGCAGTCCTTCTCCCTCTACG GCATGCAGGTCCTGCTCTTCGAGAACCAGTACTATCCCAATGGCATCCGGCTGACGTCTGCTGTCCCTGG AGCAGACATCAAAGTGCTAATAAACTTCAACGCTCCCAATCCTCAGGACCGGAAGAAGTTCACTGACGAC CTGCGGGAGTCTGTCGCTGAAGTGCAAGAGATGGAGAAACACCGAATAGAGTCGGAGCTCGAGAAGCAGA AAGGTGTCGTGCGGCCCAGCATGTCACAGTGCTCCAGCCTCAAAAAGGAGTCAGGCAACGGGACACTGAG CAGGGCTTGCCTGGATGACAGCTATGCCAGTGGCGAGGGCCTCAAGCGCAGCGCCCTCAGCAGCTCACTG AGAGACCTCTCAGAAGCGGGGAAGCGAGGGCGTCGCAGCAGTGCGGGATCGCTAGAGAGCAATGTGGAAG GCTCCATCATTAGCAGTCCTCACATGCGCCGGAGAGCGACCTCAACACGAGAGTGCCCATCTCGCCCCCC CCAGCCCGTGCCTAACTCCTCCTCTCTCCTGGGTTCTTTGTTTGGGAGCAGGAGAGGGAAGCCCCCTCCC CAGGCCCACCCACCCTCAGCCCCTCCCCCAGCACCCCCTCACGCCCATCACCCTGGGCCCTCAGAAGGCC CACTGCATGGACATCATGCACAGTACTGCCATGCACAGCAGAATCCTCCGCCCTATCACCACCACCACCA CTACCACCCACCCCCCCACCTGCAGCACACCCACCAGTACCACCATGGCCCACACGGCGGGCACCCCCCG TATGGGGCCCATGCACACAGCCACCCGCCTCTGCCCACAGCTCACCCTGGTCATGCAGGGCACTCAGCGC ATCATCATGGGCAGCCCCCTGCCCCACCGCCCCCCACCAGCAGCAAGGCCAAGCCCAGCGGCATCAGCAC AGTTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227689 representing NM_001134384
Red=Cloning site Green=Tags(s) MACRRRYFVEGEAPSSETGTSLDSPSAYHQGPLVPGSSLSPDHYEHTSVGAYGLYAGPGPQQRTRRPRLQ HSTSVLRKQAEEEAIKRSRSLSESYELSSDLQDKQVEMLERKYGGRLVTRHAARTIQTAFRQYQMNKNFE RLRSSMSENRMSRRIVLSNMRMQFSFEGPEKVHSSYFEGKQVSVTNDGSQLGALVPSECGDLSDPALKSP APSSDFADAITELEDAFSRQVKSLAESIDDALNCRSLHSEEVPASDTARARDTEPKPGLHGMDHRKLDEM TASYSDVTLYIDEEELSPPLPLSQAGDRPSSTESDLRLRSGGAAQDYWALAHKEDKADTDTSCRSTPSLE RPEPRLRVEHLPLLTIEPPSDSSVELSDRSDRSSLKRQSAYERSLGGQQGSPKHGPHGGPPKGLPREEPE LRPRPPRPLESHLAINGSANRQSKSESDYSDGDNDSINSTSNSNDTINCSSESSSRDSLREQTLSKQTYH KETRNSWDSPAFSNDVIRKRHYRIGLNLFNKKPEKGIQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGE FLGNRQKQFNRDVLDCVVDEMDFSAMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCVCNPGVVRQF RNPDTIFILAFAIILLNTDMYSPNVKPERKMKLEDFVKNLRGVDDGEDIPRETLIGIYERIRKRELKTNE DHVSQVQKVEKLIVGKKPIGSLHHGLGCVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLV VTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLTSAVPGADIKVLINFNAPNPQDRKKFTDD LRESVAEVQEMEKHRIESELEKQKGVVRPSMSQCSSLKKESGNGTLSRACLDDSYASGEGLKRSALSSSL RDLSEAGKRGRRSSAGSLESNVEGSIISSPHMRRRATSTRECPSRPPQPVPNSSSLLGSLFGSRRGKPPP QAHPPSAPPPAPPHAHHPGPSEGPLHGHHAQYCHAQQNPPPYHHHHHYHPPPHLQHTHQYHHGPHGGHPP YGAHAHSHPPLPTAHPGHAGHSAHHHGQPPAPPPPTSSKAKPSGISTVV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001134384 |
| ORF Size | 3297 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001134384.1, NP_001127856.1 |
| RefSeq Size | 6490 bp |
| RefSeq ORF | 3300 bp |
| Locus ID | 232227 |
| Gene Summary | Guanine nucleotide exchange factor for ARF1 and ARF6. Guanine nucleotide exchange factor activity is enhanced by lipid binding. Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223570 | Iqsec1 (untagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 2, (10ug) |
CNY 14,060.00 |
|
| MR227689 | Iqsec1 (Myc-DDK-tagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 2 |
CNY 7,656.00 |
|
| MR227689L3 | Lenti ORF clone of Iqsec1 (Myc-DDK-tagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 2 |
CNY 11,500.00 |
|
| MR227689L4 | Lenti ORF clone of Iqsec1 (mGFP-tagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 2 |
CNY 11,500.00 |
