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Pmm1 (NM_013872) Mouse Tagged ORF Clone
CAT＃: MG227540
- TrueORF®
Pmm1 (tGFP-tagged) - Mouse phosphomannomutase 1 (Pmm1), (10ug)
ORF Plasmid: DDK
"NM_013872" in other vectors (4)
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CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C77612 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227540 representing NM_013872
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTCGCCGTCGAGGGCGCCCGCAGGAAAGAGCGCATCCTCTGCCTGTTTGACGTGGACGGGACCC TCACGCCAGCTCGCCAGAAAATTGACCCTGAGGTATCGGCCTTCCTGCAGAAGCTACGAAGCAGGGTACA GATCGGCGTGGTGGGTGGCTCTGACTACTCTAAGATCGCCGAGCAGCTGGGAGAGGGGGATGAAGTCATT GAGAAGTTTGACTACGTGTTTGCTGAGAATGGGACAGTGCAGTATAAACACGGACGGCTACTCTCCAAAC AGACGATCCAGAACCACCTGGGGGAGGAGCTCCTGCAGGACTTGATCAACTTCTGCCTTAGCTACATGGC CCTGCTCAGACTGCCCAAGAAGCGTGGGACCTTCATTGAGTTCCGGAATGGCATGCTGAACGTCTCGCCC ATTGGCCGCAGCTGCACCCTGGAGGAGAGGATCGAGTTCTCGGAACTGGACAAGAAGGAGAAGATCCGGG AGAAGTTTGTGGAAGCCTTGAAGACAGAGTTTGCTGGCAAGGGGCTGCGGTTCTCCCGAGGAGGCATGAT AAGCTTCGATGTCTTCCCCGAGGGCTGGGATAAGCGCTACTGCCTGGACAGCCTGGATGAAGACAGCTTT GACATCATCCACTTCTTTGGAAATGAGACCAGTCCTGGCGGGAATGACTTTGAGATCTATGCGGACCCCC GGACTGTCGGCCATAGCGTGGTCTCCCCTCAGGACACTGTACAGCGATGCCGTGAGCTCTTCTTCCCAGA GACAGCCCACGAGGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227540 representing NM_013872
Red=Cloning site Green=Tags(s) MAVAVEGARRKERILCLFDVDGTLTPARQKIDPEVSAFLQKLRSRVQIGVVGGSDYSKIAEQLGEGDEVI EKFDYVFAENGTVQYKHGRLLSKQTIQNHLGEELLQDLINFCLSYMALLRLPKKRGTFIEFRNGMLNVSP IGRSCTLEERIEFSELDKKEKIREKFVEALKTEFAGKGLRFSRGGMISFDVFPEGWDKRYCLDSLDEDSF DIIHFFGNETSPGGNDFEIYADPRTVGHSVVSPQDTVQRCRELFFPETAHEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013872 |
| ORF Size | 786 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013872.4, NP_038900.1 |
| RefSeq Size | 1239 bp |
| RefSeq ORF | 789 bp |
| Locus ID | 29858 |
| UniProt ID | O35621 |
| Gene Summary | Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209823 | Pmm1 (untagged) - Mouse phosphomannomutase 1 (Pmm1), (10ug) |
CNY 3,990.00 |
|
| MR227540 | Pmm1 (Myc-DDK-tagged) - Mouse phosphomannomutase 1 (Pmm1) |
CNY 2,660.00 |
|
| MR227540L3 | Lenti ORF clone of Pmm1 (Myc-DDK-tagged) - Mouse phosphomannomutase 1 (Pmm1) |
CNY 4,560.00 |
|
| MR227540L4 | Lenti ORF clone of Pmm1 (mGFP-tagged) - Mouse phosphomannomutase 1 (Pmm1) |
CNY 4,560.00 |
