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Gja3 (NM_016975) Mouse Tagged ORF Clone
CAT＃: MG226893
- TrueORF®
Gja3 (tGFP-tagged) - Mouse gap junction protein alpha 3 (Gja3), (10ug)
ORF Plasmid: DDK
"NM_016975" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cnx46; Cx43; Cx46; Gja-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226893 representing NM_016975
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACTGGAGCTTCCTGGGGCGGCTGCTGGAGAACGCACAGGAGCACTCTACAGTCATCGGCAAAG TGTGGCTGACCGTGCTGTTCATCTTCCGCATTCTGGTGTTAGGGGCGGCAGCCGAGGAGGTGTGGGGCGA CGAGCAATCGGACTTCACCTGCAACACACAGCAGCCAGGCTGTGAGAACGTCTGCTACGACCGCGCTTTC CCCATTTCGCACATCCGCTTCTGGGCGCTGCAAATCATCTTCGTGTCTACGCCCACCCTCATCTATCTGG GCCACGTGCTACACATCGTGCGCATGGAGGAGAAGAAGAAAGAGCGGGAGGAAGAGCTGCTGAGGAGAGA CAACCCTCAGCACGGCCGTGGTCGCGAGCCAATGCGTACAGGGAGCCCGCGGGACCCTCCACTACGCGAT GACCGTGGCAAGGTGCGCATCGCAGGTGCGCTGCTGCGGACCTACGTCTTCAACATCATCTTCAAGACAC TCTTCGAAGTGGGGTTCATCGCGGGCCAGTACTTTCTATACGGCTTCCAGCTGCAGCCACTTTACCGCTG CGACCGCTGGCCCTGCCCCAACACTGTGGACTGTTTCATCTCCAGGCCCACAGAGAAGACCATCTTTGTC ATCTTCATGCTGGCTGTGGCCTGTGCGTCACTGGTACTCAACATGCTGGAGATTTACCACCTGGGCTGGA AGAAGCTCAAGCAGGGAGTTACTAACCACTTCAACCCAGATGCCTCAGAAGCCAGGCACAAGCCCTTGGA CCCCCTACCCACGGCCACCAGCTCTGGCCCGCCCAGCGTCTCCATCGGGTTCCCACCTTATTACACACAC CCTGCCTGTCCCACAGTACAGGCAAAGGCCATAGGGTTTCCTGGGGCCCCACTATCACCAGCAGACTTCA CAGTGGTGACTCTAAACGATGCTCAAGGCAGAAACCACCCAGTCAAACACTGCAATGGCCACCACCTGAC GACAGAGCAGAACTGGACCAGGCAAGTGGCAGAGCAGCAGACTCCAGCCAGCAAGCCCTCTTCAGCAGCA TCCAGCCCTGATGGCCGCAAGGGGCTCATTGACAGCAGTGGCAGCAGCTTACAGGAGAGTGCCTTGGTAG TGACGCCAGAGGAGGGGGAACAGGCTTTGGCCACCACAGTGGAGATGCACTCGCCACCGTTGGTCCTCCT GGACCCAGGAAGGTCCAGCAAGTCCAGCAACGGACGTGCCAGACCAGGTGACTTGGCCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226893 representing NM_016975
Red=Cloning site Green=Tags(s) MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEEVWGDEQSDFTCNTQQPGCENVCYDRAF PISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEELLRRDNPQHGRGREPMRTGSPRDPPLRD DRGKVRIAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFQLQPLYRCDRWPCPNTVDCFISRPTEKTIFV IFMLAVACASLVLNMLEIYHLGWKKLKQGVTNHFNPDASEARHKPLDPLPTATSSGPPSVSIGFPPYYTH PACPTVQAKAIGFPGAPLSPADFTVVTLNDAQGRNHPVKHCNGHHLTTEQNWTRQVAEQQTPASKPSSAA SSPDGRKGLIDSSGSSLQESALVVTPEEGEQALATTVEMHSPPLVLLDPGRSSKSSNGRARPGDLAI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016975 |
| ORF Size | 1251 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016975.3, NP_058671.2 |
| RefSeq Size | 1411 bp |
| RefSeq ORF | 1254 bp |
| Locus ID | 14611 |
| UniProt ID | Q64448 |
| Gene Summary | Structural component of lens fiber gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208554 | Gja3 (untagged) - Mouse gap junction protein, alpha 3 (Gja3), (10ug) |
CNY 3,990.00 |
|
| MR226893 | Gja3 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 3 (Gja3) |
CNY 2,850.00 |
|
| MR226893L3 | Lenti ORF clone of Gja3 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 3 (Gja3) |
CNY 4,750.00 |
|
| MR226893L4 | Lenti ORF clone of Gja3 (mGFP-tagged) - Mouse gap junction protein, alpha 3 (Gja3) |
CNY 4,750.00 |
