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Srd5a3 (NM_020611) Mouse Tagged ORF Clone
CAT＃: MG226010
- TrueORF®
Srd5a3 (tGFP-tagged) - Mouse steroid 5 alpha-reductase 3 (Srd5a3), (10ug)
ORF Plasmid: DDK
"NM_020611" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110025P14Rik; A430076C09; AV364670; AW987574; D730040M03Rik; H5ar; S5AR 3; Srd5a2l |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226010 representing NM_020611
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGTGGGCCGGGTTTGAGCTCTCGGCCCTGAACCCCCTGCGGACGCTGTGGCTGGCGCTGGCCG CCGCCTTCCTGTTCGCGCTGCTGCTGCAGCTGGCGCCCGCCAGGCTGCTGCCGAGCTGCGCGCTCTTCCA GGACCTGCTCCGCTACGGGAAGACCAAGCAGTCCGGCTCGCGGCGCCCCGCCGTCTGCAGGGCCTTCGAT GTCCCCAAGAGGTACTTTTCTCACTTCTACGTCATCTCAGTTGTGTGGAATGGCTCCCTGCTCTGGCTAC TTTCTCAGTCGTTGTTCCTGGGAGCACCTTTTCCAAACTGGCTTAGTGCTCTGCTCAGAACTCTTGGGGC CACACAGTTCCAAGCCCTGGAGATGGAGTCCAAGGCTTCTCGGATGCCAGCGGCTGAGCTGGCTCTGTCT GCCTTCTTGGTCTTGGTGTTCCTCTGGGTCCACAGCCTTCGGAGACTCTTTGAGTGCTTCTACGTCAGTG TCTTCTCTAATGCAGCCATTCACGTTGTGCAGTACTGTTTCGGGCTTGTCTACTATGTGCTTGTTGGCCT GACTGTACTGAGCCAAGTGCCCATGGATGATAAGAATGTGTATGTTCTGGGGAAGAATCTACTGATACAA GCCCGGTGGTTCCACATCCTGGGCATGGTGATGTTCTTCTGGTCATCCGCCCATCAGTATAAATGCCACG TCATCCTCAGCAACCTCAGGAGAAACAAAAAAGGTGTGGTCATCCACTGCCAGCACCGGATCCCCTTTGG AGACTGGTTCGAGTACGTGTCTTCAGCTAACTACCTAGCAGAGCTGATGATCTACATCTCCATGGCTGTC ACCTTTGGGCTCCACAACTTAACCTGGTGGCTGGTGGTGACGTATGTCTTCTCCAGCCAAGCCTTGTCCG CATTCTTCAACCACAAGTTCTACAGAAGCACATTTGTCTCCTACCCAAAGCATAGGAAAGCGTTCCTCCC ATTTTTGTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226010 representing NM_020611
Red=Cloning site Green=Tags(s) MAGWAGFELSALNPLRTLWLALAAAFLFALLLQLAPARLLPSCALFQDLLRYGKTKQSGSRRPAVCRAFD VPKRYFSHFYVISVVWNGSLLWLLSQSLFLGAPFPNWLSALLRTLGATQFQALEMESKASRMPAAELALS AFLVLVFLWVHSLRRLFECFYVSVFSNAAIHVVQYCFGLVYYVLVGLTVLSQVPMDDKNVYVLGKNLLIQ ARWFHILGMVMFFWSSAHQYKCHVILSNLRRNKKGVVIHCQHRIPFGDWFEYVSSANYLAELMIYISMAV TFGLHNLTWWLVVTYVFSSQALSAFFNHKFYRSTFVSYPKHRKAFLPFLF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020611 |
| ORF Size | 990 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020611.4, NP_065636.2 |
| RefSeq Size | 1733 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 57357 |
| UniProt ID | Q9WUP4 |
| Gene Summary | Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210140 | Srd5a3 (untagged) - Mouse steroid 5 alpha-reductase 3 (Srd5a3), (10ug) |
CNY 3,990.00 |
|
| MR226010 | Srd5a3 (Myc-DDK-tagged) - Mouse steroid 5 alpha-reductase 3 (Srd5a3) |
CNY 1,416.00 |
|
| MR226010L3 | Lenti ORF clone of Srd5a3 (Myc-DDK-tagged) - Mouse steroid 5 alpha-reductase 3 (Srd5a3) |
CNY 4,750.00 |
|
| MR226010L4 | Lenti ORF clone of Srd5a3 (mGFP-tagged) - Mouse steroid 5 alpha-reductase 3 (Srd5a3) |
CNY 4,750.00 |
