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Trpm7 (NM_021450) Mouse Tagged ORF Clone
CAT＃: MG224982
- TrueORF®
Trpm7 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 7 (Trpm7) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_021450" in other vectors (2)
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CNY 20,520.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224982 representing NM_021450
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGAAATCCTGGATAGAGAGCACTTTGACCAAGAGGGAGTGTGTATATATTATACCAAGCTCCA AAGACCCTCACAGATGTCTTCCAGGATGTCAGATTTGTCAGCAACTTGTCAGATGTTTCTGTGGTCGTTT GGTCAAGCAACATGCATGCTTTACTGCAAGTCTTGCCATGAAATACTCAGATGTGAAATTGGGTGAACAC TTTAACCAGGCAATAGAAGAATGGTCTGTGGAAAAGCACACGGAGCAGAGCCCAACAGATGCTTATGGAG TCATCAATTTTCAAGGGGGTTCTCATTCCTACAGAGCTAAGTATGTGAGACTATCATATGATACCAAACC TGAAATCATTCTGCAACTTCTGCTTAAAGAATGGCAAATGGAGTTACCCAAACTTGTTATTTCTGTACAT GGAGGCATGCAGAAGTTTGAACTTCATCCAAGAATCAAGCAGTTGCTTGGAAAGGGTCTTATTAAAGCTG CAGTTACAACCGGAGCTTGGATTTTAACTGGAGGAGTCAATACAGGTGTGGCAAAACATGTTGGTGATGC CCTCAAAGAACATGCTTCCAGATCATCTCGAAAAATTTGCACTATTGGAATAGCTCCATGGGGAGTGATA GAAAACAGAAATGATCTTGTTGGGAGAGATGTGGTTGCTCCTTATCAAACCCTATTGAATCCCTTGAGCA AATTGAATGTTCTGAATAATCTACACTCCCATTTCATCTTGGTGGATGATGGCACTGTTGGAAAGTATGG GGCAGAAGTCAGACTGAGAAGAGAACTTGAAAAAACCATTAATCAGCAAAGAATTCATGCTAGAATTGGG CAAGGAGTTCCTGTGGTGGCTTTGATATTTGAAGGCGGGCCAAATGTCATCCTTACAGTACTGGAGTACC TTCAGGAAAGCCCCCCAGTTCCAGTTGTTGTGTGTGAAGGGACAGGCAGAGCTGCAGATTTACTAGCCTA TATCCACAAACAGACAGAGGAAGGAGGAAATCTTCCTGATGCAGCAGAGCCTGATATTATATCAACTATC AAGAAAACATTTAACTTTGGCCAGAGTGAAGCAGTTCATTTATTTCAAACAATGATGGAGTGTATGAAAA AAAAAGAGCTTATCACTGTTTTTCACATTGGATCAGAGGATCATCAAGATATAGATGTGGCCATACTCAC TGCACTGCTGAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACACTGGCATGGGACAGAGTT TATATTGCCAAAAATCATGTATTTGTTTATGGACAACAGTGGCTGGTTGGATCCTTGGAACAGGCTATGC TTGATGCTCTTGTAATGGACAGAGTTTCATTTGTAAAACTTCTTATTGAAAACGGAGTAAGCATGCATAA ATTCCTTACCATTCCCAGACTGGAAGAACTTTATAACACTAAACAAGGTCCAACCAATCCAATGTTGTTC CATCTCATTCGGGATGTCAAGCAGGGTAATCTCCCCCCGGGGTACAAGATCACTTTAATTGATATAGGAC TTGTGATTGAGTATCTCATGGGAGGAACCTACAGATGCACATACACACGAAAACGTTTTCGATTGATATA TAATAGTCTTGGTGGAAATAACCGGAGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAG AGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAG CCCAACCCTACAGACCAAAGATGGATGCATCTATGGAAGAAGGAAAGAAGAAAAGAACCAAAGATGAAAT TGTAGATATAGATGATCCAGAGACCAAGCGCTTTCCTTATCCTCTTAATGAATTATTAATTTGGGCTTGC CTTATGAAGAGGCAGGTCATGGCCCGCTTTTTATGGCAGCATGGTGAAGAATCAATGGCTAAAGCATTAG TTGCCTGTAAAATCTATCGTTCAATGGCTTATGAGGCAAAGCAGAGTGACCTGGTAGATGATACTTCAGA GGAACTGAAGCAGTATTCCAATGATTTTGGCCAACTGGCAGTTGAATTACTGGAACAGTCCTTCAGACAG GATGAAACGATGGCTATGAAATTACTCACTTATGAACTCAAAAACTGGAGTAATTCAACCTGCCTCAAGT TAGCAGTTTCTTCAAGACTTAGACCTTTTGTAGCTCACACTTGTACACAGATGTTGTTATCTGATATGTG GATGGGACGGCTGAATATGAGAAAAAATTCCTGGTATAAGGTCATATTAAGCATTTTAGTTCCACCTGCC ATATTAATGCTAGAGTATAAAACCAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAAA TGACGATGGAGGATAGTGAAAACAATTTTCACAACATAACAGAAGAGATACCCATGGAAGTATTTAAAGA AGTAAAGATTTTGGACAGCAGTGATGGAAAGAATGAAATGGAGATACATATTAAATCAAAAAAGCTTCCA ATCACACGAAAATTTTATGCCTTTTATCATGCACCAATTGTAAAGTTCTGGTTTAACACATTGGCATATT TAGGATTTCTGATGCTTTATACATTTGTAGTTCTTGTAAAAATGGAACAGTTACCTTCAGTTCAAGAATG GATTGTTATCGCTTATATTTTTACCTATGCTATTGAAAAAGTCCGTGAGGTCTTCATGTCTGAAGCTGGG AAAATCAGCCAGAAGATTAAAGTATGGTTTAGTGACTACTTCAATGTCAGTGACACAATTGCCATCATTT CTTTCTTTGTTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTATATTAATGCATATGATAATCATGT TTTTGTGGCTGGAAGATTAATTTACTGTCTTAATATAATATTTTGGTATGTGCGTTTGCTAGACTTTCTA GCCGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTG TAGTGATAATGGCTCTTGTATTGCTTAGTTTTGGTGTTCCCAGAAAAGCAATACTTTATCCACATGAAGA ACCATCTTGGTCTCTTGCTAAAGATATAGTTTTTCATCCATACTGGATGATTTTTGGTGAAGTTTATGCA TATGAAATTGATGTGTGTGCAAATGACTCCACTCTCCCGACAATCTGTGGTCCTGGAACTTGGTTGACTC CATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTCCTTATCGCATTTTTCAA TAATGTATATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTATCAGCGGTATCATTTTATTATG GCTTATCATGAAAAACCAGTCCTGCCTCCTCCTCTTATCATCCTCAGCCATATAGTTTCACTGTTTTGCT GTGTATGCAAAAGAAGAAAGAAAGATAAGACTTCCGATGGGCCAAAACTTTTCTTAACAGAAGAAGATCA AAAGAAACTCCATGATTTTGAAGAGCAGTGTGTTGAGATGTACTTTGATGAGAAAGATGACAAATTCAAT TCTGGGAGTGAAGAGAGAATCCGGGTCACTTTTGAAAGAGTGGAGCAGATGAGCATTCAGATTAAAGAAG TTGGAGATCGTGTCAACTACATAAAAAGATCATTACAGTCTTTAGATTCTCAAATTGGTCATCTGCAAGA TCTCTCAGCCCTAACAGTAGATACATTGAAAACACTTACAGCCCAGAAAGCTTCAGAAGCTAGTAAAGTG CACAATGAGATCACACGAGAATTGAGTATTTCCAAACACTTGGCTCAGAATCTTATTGATGATGTTCCTG TAAGACCTTTGTGGAAGAAACCTAGTGCTGTAAACACACTGAGTTCCTCTCTTCCTCAAGGTGATCGGGA AAGTAATAATCCTTTTCTTTGTAATATTTTTATGAAAGATGAAAAAGACCCCCAATATAATCTGTTTGGA CAAGATTTGCCCGTGATACCCCAGAGAAAAGAATTCAACATTCCAGAGGCTGGTTCCTCCTGTGGTGCCT TATTCCCAAGTGCTGTTTCTCCCCCAGAATTACGACAGAGACGACATGGGGTAGAAATGTTAAAAATATT TAATAAAAATCAAAAATTAGGCAGTTCACCTAATAGTTCACCACATATGTCCTCCCCACCAACCAAATTT TCTGTGAGTACCCCATCCCAGCCAAGTTGCAAAAGTCACTTGGAATCCACAACCAAAGATCAAGAACCCA TTTTCTATAAAGCTGCAGAAGGGGATAACATAGAATTTGGAGCATTTGTGGGACACAGAGATAGTATGGA CTTACAGAGGTTTAAAGAAACATCAAACAAAATAAGAGAACTGTTATCTAATGATACTCCTGAAAACACT CTGAAACATGTGGGTGCTGCTGGATATAGTGAATGTTGTAAGACTTCTACTTCTCTTCACTCGGTGCAAG CAGAAAGCTGTAGTAGAAGAGCGTCGACGGAAGACTCTCCAGAAGTCGATTCTAAAGCAGCTTTGTTACC GGATTGGTTACGAGATAGACCATCAAACAGAGAAATGCCATCTGAAGGAGGAACATTAAATGGTCTTGCT TCTCCATTTAAGCCCGTTTTGGATACAAATTACTATTATTCAGCTGTGGAAAGAAATAACCTGATGAGGT TGTCACAGAGTATTCCCTTCGTTCCTGTACCTCCACGAGGCGAGCCTGTCACAGTGTACCGTCTGGAGGA GAGTTCTCCCAGTATACTGAATAACAGCATGTCTTCATGGTCTCAGCTAGGCCTCTGTGCCAAAATTGAG TTTTTAAGTAAAGAGGAAATGGGAGGTGGTTTACGAAGAGCAGTCAAAGTGCTGTGTACCTGGTCAGAGC ACGATATCCTGAAGTCAGGGCATCTCTATATCATTAAGTCATTTCTTCCTGAGGTGATAAACACATGGTC AAGCATTTATAAAGAAGATACGGTTCTACATCTCTGTCTCAGAGAAATACAACAACAGAGAGCAGCACAA AAGCTCACATTTGCCTTTAATCAGATGAAACCCAAATCCATACCATATTCTCCAAGGTTCCTTGAAGTTT TCCTGTTGTACTGCCATTCAGCAGGGCAGTGGTTTGCTGTAGAAGAGTGCATGACTGGTGAATTTAGAAA ATACAACAACAATAATGGTGATGAAATCATTCCTACAAATACTCTAGAAGAGATCATGCTAGCCTTTAGC CACTGGACCTATGAATATACCAGAGGGGAGTTACTGGTACTTGACTTACAAGGAGTGGGAGAAAACTTGA CTGACCCATCTGTAATAAAAGCTGAAGAAAAAAGATCCTGTGACATGGTTTTTGGCCCTGCCAATCTAGG AGAAGATGCAATAAAAAACTTCAGAGCCAAACATCACTGTAATTCTTGCTGTCGAAAGCTTAAACTTCCA GATTTGAAGAGGAATGACTACACGCCTGATAAAATTATATTTCCTCAGGATGAGTCATCAGATTTGAATC TTCAATCTGGAAATTCCACCAAAGAATCAGAAGCAACAAATTCTGTTCGTCTGATGTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224982 representing NM_021450
Red=Cloning site Green=Tags(s) MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGEH FNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEIILQLLLKEWQMELPKLVISVH GGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVI ENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIG QGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTI KKTFNFGQSEAVHLFQTMMECMKKKELITVFHIGSEDHQDIDVAILTALLKGTNASAFDQLILTLAWDRV YIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVSFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLF HLIRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRK SHETFGNRADKKEKMRHNHFIKTAQPYRPKMDASMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWAC LMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQ DETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPA ILMLEYKTKAEMSHIPQSQDAHQMTMEDSENNFHNITEEIPMEVFKEVKILDSSDGKNEMEIHIKSKKLP ITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVKMEQLPSVQEWIVIAYIFTYAIEKVREVFMSEAG KISQKIKVWFSDYFNVSDTIAIISFFVGFGLRFGAKWNYINAYDNHVFVAGRLIYCLNIIFWYVRLLDFL AVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEEPSWSLAKDIVFHPYWMIFGEVYA YEIDVCANDSTLPTICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIM AYHEKPVLPPPLIILSHIVSLFCCVCKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFDEKDDKFN SGSEERIRVTFERVEQMSIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKV HNEITRELSISKHLAQNLIDDVPVRPLWKKPSAVNTLSSSLPQGDRESNNPFLCNIFMKDEKDPQYNLFG QDLPVIPQRKEFNIPEAGSSCGALFPSAVSPPELRQRRHGVEMLKIFNKNQKLGSSPNSSPHMSSPPTKF SVSTPSQPSCKSHLESTTKDQEPIFYKAAEGDNIEFGAFVGHRDSMDLQRFKETSNKIRELLSNDTPENT LKHVGAAGYSECCKTSTSLHSVQAESCSRRASTEDSPEVDSKAALLPDWLRDRPSNREMPSEGGTLNGLA SPFKPVLDTNYYYSAVERNNLMRLSQSIPFVPVPPRGEPVTVYRLEESSPSILNNSMSSWSQLGLCAKIE FLSKEEMGGGLRRAVKVLCTWSEHDILKSGHLYIIKSFLPEVINTWSSIYKEDTVLHLCLREIQQQRAAQ KLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFS HWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLP DLKRNDYTPDKIIFPQDESSDLNLQSGNSTKESEATNSVRLML TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021450 |
| ORF Size | 5589 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021450.2, NP_067425.2 |
| RefSeq Size | 7145 bp |
| RefSeq ORF | 5592 bp |
| Locus ID | 58800 |
| UniProt ID | Q923J1 |
| Gene Summary | Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224988 | Trpm7 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 7 (Trpm7), transcript variant 1, (10ug) |
CNY 29,930.00 |
|
| MR224982 | Trpm7 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 7 (Trpm7), transcript variant 1 |
CNY 18,620.00 |
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