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Espn (NM_207687) Mouse Tagged ORF Clone
CAT＃: MG224951
- TrueORF®
Espn (tGFP-tagged) - Mouse espin (Espn) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_207687" in other vectors (4)
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CNY 9,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | je |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224951 representing NM_207687
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGAGCAGGCGCTGCAGGCGGCACGGCGGGGCGACCTGGACGTGCTGAGGTCCCTGCACGCCG CCGGCCTGCTGGGGCCTTCTCTGCGCGACTCGCTAGACGCCCTGCCGGTGCACCATGCGGCCCGCTCAGG CAAGCTGCACTGTCTGCGCTACTTGGTGGAGGAGGTTGCCCTCCCAGCCGTGTCCCGCGCGCGCAACGGC GCCACACCAGCCCATGACGCCGCCGCCACAGGCTACCTCTCTTGCCTGCAGTGGCTGCTCACACAGGGTG GCTGCAGGGTGCAGGAAAAAGATAACTCTGGTGCCACAGTCCTGCATCTGGCTGCCCGCTTTGGCCACCC GGATGTGGTGAAGTGGCTGCTGTACCAGGGCGGTGCAAATTCGGCCATCACCACAGACACGGGCGCCCTG CCTATCCACTACGCTGCCGCCAAAGGAGACCTCCCCTCGCTGAAGCTTCTGGTCGGGCATTACCCTGAGG GAGTGAATGCCCAAACCAACAACGGTGCCACGCCCCTGTACCTGGCGTGCCAGGAGGGCCACCTGGAAGT GACGAAGTACCTTGTGCAGGAGTGCAGTGCAGATCCGCACCTGCGCGCCCAAGACGGCATGACACCCCTA CATGCCGCAGCACAGATGGGCCACAACCCAGTCTTGGTGTGGCTGGTGAGCTTTGCAGACGTTAGCTTTT CAGAGCAGGACCACGACGGCGCCACGGCCATGCACTTTGCTGCCAGTCGCGGCCACACCAAAGTGCTCAG CTGGCTCCTGCTGCACGGGGCAGAGATCTCGCAGGACCTGTGGGGCGGAACCCCGCTGCATGACGCTGCT GAGAACGGGGAACTGGAGTGCTGCCAGATCCTCGCGGTGAACGGCGCTGGGCTGGACGTCCGCGACCACG ATGGGTACACCGCTGCCGACCTGGCAGAGTTCAATGGCCACACCCACTGTTCCCGCTACCTACGTACGGT GCAAACCCTGAGCTTGGAACACCGAGTCTTGTCCCGGGATCAATCCATGGACCTGGAGGCAAAGCAGCTG GACTCGGGTATGTCCTCGCCCAACACCACCATGTCGGTCCAGCCAATGACCTTTGACCTGGGCTCGCCTA CTAGCACGTTCTCCAACTATGACTCCTGCTCCTCCAGTCACTCCAGCAGCAAGGGGCAGCGATCGAATCG AGGGATTCCAGGTGCAAGAGCTGCAGACTTACAGAGCTACATGGACATGCTGAACCCAGAGAAGAGCTTG CCTCGGGGCAAGCTAGGGAAGCCTTCCCCGCCACCACCTCCACCACCACCACCACCAAGCTTCCCGCCAC CCCCACCACCCACAGGCACCCAGCCGCCCCCACCTCCACCAGGCTACCCAGCTCCCAATCCCCCTGTGGG ACTGCATCTGAATAACATTTACATGCAGACCAAGAACAAGCTTCGCCATGTGGAGGTGGACTCGCTCAAG GAGCCCAAGGTGGAGCTGAACGATCAGTTTGCACAGCCGAGCTCGGGCGACGGCCACTCGGGGCTACACA GGCAGGACTCCGGGCTGCTCAGGCAGGATTCGGAGCTGCTGCACAGGCAGGAGCTGCTCAGGCACAGCAC CGGACTGCGCAGGCAGGACTCCGACCGCAAACAGCGCTCGTTCAGTAAACAGCCCAGCACGGGGGACTAC TACCGCCAGCTGGGCCGCAGCCCGGGGGAGCCGCTGGCCGCACGCCCGGGCATGGCCCACAGCGAGGAGG CGGCGCTGCTCCCCGGGAACCACGTGCACAACGGCTGCTCAGCGGACTCCAAAGCGTCCAGGGAGCTGCC GCCGCCACCGCCGCCGCCGCCGCTGCCCGAGGCCCTGAGTTCGCCGCCGCCCGCCCCACCTCTGCCCATC GAGGGCGCGGGCGCAGCCTGCGGGCAGCGTCGTTCCTCGTCTTCTACTGGCAAAGTGAGAGTCCTGAGAC ACAGGAAGAGCACCAAATCTTTCAACATGATGTCCCCAACGGGTGATAACTCAGAGCTTCTGGCTGAGAT AAAGGCGGGCAAGAGCCTGAAGCCGACACCGCAGAGCAAGGGGCTGACAACCGTGTTCTCAGGCAGTGGG CAGCCAGCCTCCCAGCCTGAGTCACCGCAGCCTCTGGTGTCACCTGCGCCATCTCGGACTCGGAGCCCCA CCCCGCCAGCCTCTGGGTCTCAGCCACTGCTCAATGGCAGTGTGGTGCCGGCACCACCTGCCACCCCGGC ACCTGGAGTCCATCTGGATGTGGAGGCCCTCATTCCCACTCTTGATGAGCAGGGCCGGCCCATCCCGGAG TGGAAGCGCCAGGTGATGGTCCGCAAGCTGCAGCAGAAGATGCAGGAGGAAGAGGAGCAGCGGAGGAAGG AGGAAGAGGAGGAGGCCCGGCTCGCCAGCCTGCCTGCCTGGAGACGAGACATTCTTCGGAAGAAGCTGGA GGAGGAGAGGGAGCAGAAGCGAAAAGAGGAGGAGCGGCAAAAGCTGGAGGAAATACAGAGGGCGAAAGAA CAGTCGGAGAAGCTGCGGACACTAGGCTACGACGAAGCCAAGCTCGCGCCCTGGCAGCGACAGGTCATCT TGAAGAAGGGGGAGATCCCTAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224951 representing NM_207687
Red=Cloning site Green=Tags(s) MALEQALQAARRGDLDVLRSLHAAGLLGPSLRDSLDALPVHHAARSGKLHCLRYLVEEVALPAVSRARNG ATPAHDAAATGYLSCLQWLLTQGGCRVQEKDNSGATVLHLAARFGHPDVVKWLLYQGGANSAITTDTGAL PIHYAAAKGDLPSLKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQECSADPHLRAQDGMTPL HAAAQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAASRGHTKVLSWLLLHGAEISQDLWGGTPLHDAA ENGELECCQILAVNGAGLDVRDHDGYTAADLAEFNGHTHCSRYLRTVQTLSLEHRVLSRDQSMDLEAKQL DSGMSSPNTTMSVQPMTFDLGSPTSTFSNYDSCSSSHSSSKGQRSNRGIPGARAADLQSYMDMLNPEKSL PRGKLGKPSPPPPPPPPPPSFPPPPPPTGTQPPPPPPGYPAPNPPVGLHLNNIYMQTKNKLRHVEVDSLK EPKVELNDQFAQPSSGDGHSGLHRQDSGLLRQDSELLHRQELLRHSTGLRRQDSDRKQRSFSKQPSTGDY YRQLGRSPGEPLAARPGMAHSEEAALLPGNHVHNGCSADSKASRELPPPPPPPPLPEALSSPPPAPPLPI EGAGAACGQRRSSSSTGKVRVLRHRKSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSG QPASQPESPQPLVSPAPSRTRSPTPPASGSQPLLNGSVVPAPPATPAPGVHLDVEALIPTLDEQGRPIPE WKRQVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQKLEEIQRAKE QSEKLRTLGYDEAKLAPWQRQVILKKGEIPK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_207687 |
| ORF Size | 2613 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_207687.3 |
| RefSeq Size | 2861 bp |
| RefSeq ORF | 2616 bp |
| Locus ID | 56226 |
| UniProt ID | Q9ET47 |
| Gene Summary | Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimension, dynamics and signaling capacities of the actin filament-rich microvilli in the mechanosensory and chemosensory cells (PubMed:14657236, PubMed:15190118). Required for the assembly and stabilization of the stereociliary parallel actin bundles. Plays a crucial role in the formation and maintenance of inner ear hair cell stereocilia (PubMed:21455486). Involved in the elongation of actin in stereocilia (PubMed:19287378, PubMed:22264607). In extrastriolar hair cells, required for targeting MYO3B to stereocilia tips, and for regulation of stereocilia diameter and staircase formation (PubMed:26926603).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222326 | Espn (untagged) - Mouse espin (Espn), transcript variant 1, (10ug) |
CNY 11,210.00 |
|
| MR224951 | Espn (Myc-DDK-tagged) - Mouse espin (Espn), transcript variant 1 |
CNY 9,576.00 |
|
| MR224951L3 | Lenti ORF clone of Espn (Myc-DDK-tagged) - Mouse espin (Espn), transcript variant 1 |
CNY 10,640.00 |
|
| MR224951L4 | Lenti ORF clone of Espn (mGFP-tagged) - Mouse espin (Espn), transcript variant 1 |
CNY 10,640.00 |
