Synrg (NM_001115009) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AF007009; Ap1gbp1; C76297; L71-5; SYNG
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG224023 representing NM_001115009 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCGGCCGGGAGCCGGAGCCAGCGGCGCGGCGGGGGCGGGCGCGGGGCCCGGCGGGGCAGGCA GCTTCATGTTTCCTGTTGCAGGCGGAATGAGACCCCCTCAAGCAGGCCTGATTCCAATGCAACAGCAAGG ATTCCCTATGGTCTCTGTCATGCAGCCCAATATGCAAGGCATGATGGGAATGAATTACAGCTCTCAGATG TCCCAAGGACCGATTGCAATGCAGGCAGGAATACCAATGGGGCCAATGCCAGCAGCGGGTGTGCCTTTCC TGGGTCAGCCACCTTTCTTGAGCATGCGTCCTGCAGGCCCACAGTACACTCCAGACATGCAGAAGCAGTT TGCGGAAGAGCAACAAAAACGATTTGAACAACAACAAAAGCTCTTAGAAGAAGAAAGAAAAAGACGTCAG TTTGAAGAGCAAAAGCAAAAGCTCAGACTTCTCAGCAGCGTGAAGCCCAAGACAGGAGAGAAGAACAGAG ACGATGCTTTGGAAGCCATAAAAGGAAACTTGGATGGGTTTTCCAGAGATGCTAAGATGCACCCCACTCC AGCATCACATCCAAAGAAACAAGGCCCTTCCTTGGAAGAGAAGCTCCTAGTGTCTTGTGATGTAAGTGCA TCCGGGCAGGAACACATAAAATTAAACACTCCTGATGCTGGGCACAAAGCCATAGTCCCAGGATCCAGTA AGAACTGCCCTGGTTTAATGGCCCATAACAGGGGTGCTGTGGATGGATGTGTGAGTGGTCCCGCCAGTGC AGAGGCAGAAAAGACTTCAGATCAAACCCTGTCAAAGGAAGAGAGTGGTGTGGGCGTGTTTCCCTCTCAG GACCCTGCTCAGTCCAGAATGCCTCCTTGGATTTACAATGAGAGTTTGGTTCCAGATGCCTATAAGAAAA TTTTAGAAACCACAATGACTCCAACTGGAATAGATACTGCTAAACTTTATCCCATTCTGATGTCATCTGG ACTTCCCAGGGAAACTCTCGGACAGATATGGGCCTTAGCTAATCGAACCACACCTGGCAGGCTCACTAAG GAGGAGCTTTACACGGTTCTCGCCATGGTAGCAGTAACACAGAGGGGTGTTCCTGCCATGAGCCCTGATG CTTTGAGCCAGTTCCCTGCGGCTCCCATCCCTACCTTAAGCGGCTTTCCTATGACTCTGCCTACCCCAGT GAGCCAGCCAACGGCGATGCCCTCTGGCCCTACAGGCTCCATGCCCCTCACCCTTGGACAACCCATCATG GGTATCAACCTTGTTGGACCAGTGGGTGGAGCTGCAGCACCAACTTCCAGTGGCTTCATGCCAGCCTACC CATCAAACCAGGTGGGAAAGACAGAAGAAGATGACTTCCAGGATTTTCAAGATGCTTCGAAATCAGGATC CATCGATGATTCCTTCACTGACTTCCAAGAGATGCCTGCTTCCTCAAAAACCAGTAATTCCCAGCATGGA AACAGTGCTCCATCACTCTTGATCCCATTTCCTGGAACTAAAGCCTCCACAGACAAATACGCAGTGTTCA AAGGGATTTCCACTGACAAGCCCTCTGAAAACCCTGCTTCGTTTGGAGAGTCTGGTGATAAATACAGTGC TTTCAGGGAACTCGAGCAAACGACAGACAGTAAACCTTTAGGAGAGAGCTTTGCCGAGTTCAGATCCACA GGAACCGATGATGGCTTCACTGACTTCAAGACCGCCGATAGTGTGTCACCCCTAGAGCCACCCACAAAAG ACACTTTCCCATCAGCTTTTGCCTCAGGAGCTGCACAGCAGACACAGACACAGGTGAAAACCCCTCTGAA CTTAGAAGATCTAGATATGTTCTCCTCAGTGGATTGCAGTGGCGAGAAACAGGTGCCCTTTTCAGCTACA TTTAGCACAGCAAAGTCCGTTTCCACTCGACCTCAGCCAGCGGGCTCTGCAGCAGCGTCAGCAGCACTGG CATCCACTAAAACTTCTAGTTTGGCAGATGATTTTGGAGAGTTCAACCTCTTTGGGGAGTATTCAAACCC TGCATCTGCTGGGGAGCAGGACGACTTTGCAGATTTTATGGCTTTCGGTAACAGCTCCATTTCATCTGAG CCAAAAGCAAGTGACAAGTATGAAGCCCTCAGGGAGGAAGTGAGTCCCAGCCCCTTGTCCAGCAGCACTG TGGAAGGGGCACAGCACCCACCTGCTGCGGCAACCAAGTATGATGTCTTCAAACAGCTGTCCCTAGAGGG CGCTGGGCTGGCCATGGAGGAGTTCAAAGAGAACACTTCCTCCACCAAGAGTGAAGATGACTTTGCCGAC TTCCACTCCAGTAAATTCTCATCCACGAGCTCAGACAAATCCCTGGGAGAGAAAGCCGTGGCTTTCAGAC ATGCCAAAGAAGACTCCTCCTCGGTGAAGTCTTTAGACCTCCCCTCCATCGGTGGCAGCAGTGTTGGCAA GGAGGACTCTGAAGATGCACTTTCTGTTCAATTTGACATGAAATTGGCTGATGTGGGAGGAGATCTTAAG CATGTCATGTCTGATAGCTCTTTGGATTTACCAACAGTTAGTGGCCAGCATCCTCCTGCCGCAGATACAG AGGACTTAAGCTGTGCTGCTTTTGGAAGCTGTAGTAGCCATTTTACAGTGAGCACACTTACAAGCTGTGA ATGGTCAGACAGGGCTGATGCACTTCAGGGCAGAAAACTCTCTCCATTTGTCCTCTCAGCAGGAAGTAGG TCCTTTTCAGCCACCTCAAACCTTCACACGAAAGAGATCTCATTTGGCAGCTCTGAAAACATCACCATGT CATCTCTCTCCAAAGGATCGGCCTTGGCCAGTGAGGACGCTCTTCCAGAGACTGCCTTCCCAGCTTTTGC CAGCTTTAAAGACATGATGCCTCAGACCACTGAGCAGAAAGAGTTTGAAAGTGGGGACTTCCAGGATTTC ACCAGGCAGGACATGCCCACGGTGGACCGCAGCCAAGAAACCTCATGTCCAAGCCCGGCTTCCAGTGTGG CTTCGCACGAGACCCCCAAGGAGGGTGCAGACGACTTTGGAGAATTTCAAAGTGAAAAGTCCAAAATCAG CAAGTTTGACTTCTTAGTAGCTAATTCCCAGAGCAAAATGAAATCCAGTGAAGAAATGATCAAAAGTGAG CTGGCAACCTTTGACCTTTCTGTTCAAGGATCACACAAGAGGAGCTTGAGCCTTGGGGATAAAGAAATAA GCCGGTCCTCTCCTTCCCCGGCCCTGGAGCAGCCCTTCAGAGACCGGTCCAACACTCTGAGTGAGAGGGC TGCGCTGCCCGTCATCCGAGACAAGTACAAGGATCTCACTGGCGAGGTGGAGGAAAATGAGAGATACGCG TATGAGTGGCAGAGATGCCTAGGGAGCGCGCTGGATGTCATTAAGAAGGCAAATGACACCTTAAATGGCA TTAGCAGCAGTGCTGTTTGCACAGAAGTTATCCAGTCGGCTCAAGGCATGGAGTATCTTCTAGGTGTGGT GGAAGTGTACCGCGTAACCAAGCGTGTGGAGCTGGGGATAAAAGCCACTGCTGTGTGTAGTGAGAAACTC CAGCAGTTGCTGAAGGACATCGATAAAGTGTGGAACAACTTAATCGGCTTCATGTCACTTGCCACACTCA CACCAGATGAGAATTCACTGGATTTTTCCTCCTGTATGTTACGGCCCGGGATCAAAAACGCTCAGGAGCT GGCTTGTGGAGTGTGTCTCTTAAATGTGGACTCCAGGAGCCGGAAAGAAGAGACGCCTGCAGAAGAACAG CCTAAAAAAGCATTCAACTCGGAGACAGACAGCTTCAAGCTGGCCTACGGAGGACACCAGTACCACGCCA GCTGTGCCAACTTCTGGATCAACTGTGTTGAACCCAAGCCTCCCGGCCTCCTCTTGCCAGATCTGCTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG224023 representing NM_001115009 Red=Cloning site Green=Tags(s) MALRPGAGASGAAGAGAGPGGAGSFMFPVAGGMRPPQAGLIPMQQQGFPMVSVMQPNMQGMMGMNYSSQM SQGPIAMQAGIPMGPMPAAGVPFLGQPPFLSMRPAGPQYTPDMQKQFAEEQQKRFEQQQKLLEEERKRRQ FEEQKQKLRLLSSVKPKTGEKNRDDALEAIKGNLDGFSRDAKMHPTPASHPKKQGPSLEEKLLVSCDVSA SGQEHIKLNTPDAGHKAIVPGSSKNCPGLMAHNRGAVDGCVSGPASAEAEKTSDQTLSKEESGVGVFPSQ DPAQSRMPPWIYNESLVPDAYKKILETTMTPTGIDTAKLYPILMSSGLPRETLGQIWALANRTTPGRLTK EELYTVLAMVAVTQRGVPAMSPDALSQFPAAPIPTLSGFPMTLPTPVSQPTAMPSGPTGSMPLTLGQPIM GINLVGPVGGAAAPTSSGFMPAYPSNQVGKTEEDDFQDFQDASKSGSIDDSFTDFQEMPASSKTSNSQHG NSAPSLLIPFPGTKASTDKYAVFKGISTDKPSENPASFGESGDKYSAFRELEQTTDSKPLGESFAEFRST GTDDGFTDFKTADSVSPLEPPTKDTFPSAFASGAAQQTQTQVKTPLNLEDLDMFSSVDCSGEKQVPFSAT FSTAKSVSTRPQPAGSAAASAALASTKTSSLADDFGEFNLFGEYSNPASAGEQDDFADFMAFGNSSISSE PKASDKYEALREEVSPSPLSSSTVEGAQHPPAAATKYDVFKQLSLEGAGLAMEEFKENTSSTKSEDDFAD FHSSKFSSTSSDKSLGEKAVAFRHAKEDSSSVKSLDLPSIGGSSVGKEDSEDALSVQFDMKLADVGGDLK HVMSDSSLDLPTVSGQHPPAADTEDLSCAAFGSCSSHFTVSTLTSCEWSDRADALQGRKLSPFVLSAGSR SFSATSNLHTKEISFGSSENITMSSLSKGSALASEDALPETAFPAFASFKDMMPQTTEQKEFESGDFQDF TRQDMPTVDRSQETSCPSPASSVASHETPKEGADDFGEFQSEKSKISKFDFLVANSQSKMKSSEEMIKSE LATFDLSVQGSHKRSLSLGDKEISRSSPSPALEQPFRDRSNTLSERAALPVIRDKYKDLTGEVEENERYA YEWQRCLGSALDVIKKANDTLNGISSSAVCTEVIQSAQGMEYLLGVVEVYRVTKRVELGIKATAVCSEKL QQLLKDIDKVWNNLIGFMSLATLTPDENSLDFSSCMLRPGIKNAQELACGVCLLNVDSRSRKEETPAEEQ PKKAFNSETDSFKLAYGGHQYHASCANFWINCVEPKPPGLLLPDLL SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001115009
ORF Size	3918 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001115009.1, NP_001108481.1
RefSeq Size	3995 bp
RefSeq ORF	3921 bp
Locus ID	217030
Gene Summary	May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins (By similarity).[UniProtKB/Swiss-Prot Function]

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