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Ghrh (NM_010285) Mouse Tagged ORF Clone
CAT＃: MG223015
- TrueORF®
Ghrh (tGFP-tagged) - Mouse growth hormone releasing hormone (Ghrh), (10ug)
ORF Plasmid: DDK
"NM_010285" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ghr; Ghrf; GRF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223015 representing NM_010285
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTCTGGGTGCTCTTTGTGATCCTCATCCTCACCAGTGGCTCCCACTGCTCACTGCCCCCCTCAC CTCCCTTCAGGATGCAGCGACACGTAGATGCCATCTTCACCACCAACTACAGGAAACTCCTGAGCCAGCT GTATGCCCGGAAAGTGATCCAGGACATCATGAACAAGCAAGGGGAGAGGATCCAGGAACAAAGGGCCAGG CTCAGCCGCCAGGAAGACAGCATGTGGACAGAGGACAAGCAGATGACCCTGGAGAGCATCTTGCAGGGAT TCCCAAGGATGAAGCCTTCAGCGGACGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223015 representing NM_010285
Red=Cloning site Green=Tags(s) MLLWVLFVILILTSGSHCSLPPSPPFRMQRHVDAIFTTNYRKLLSQLYARKVIQDIMNKQGERIQEQRAR LSRQEDSMWTEDKQMTLESILQGFPRMKPSADA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010285 |
| ORF Size | 309 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010285.3 |
| RefSeq Size | 534 bp |
| RefSeq ORF | 312 bp |
| Locus ID | 14601 |
| UniProt ID | P16043 |
| Gene Summary | This gene encodes a hormone that has stimulatory effects on pituitary growth hormone synthesis and release, and somatotrope expansion. The encoded preproprotein undergoes proteolytic processing to generate the mature peptide that is secreted by hypothalamus. Mice lacking the encoded protein are deficient in the growth hormone, live longer and exhibit growth retardation, enhanced insulin sensitivity and increased xenobiotic metabolism. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208549 | Ghrh (untagged) - Mouse growth hormone releasing hormone (Ghrh), (10ug) |
CNY 3,990.00 |
|
| MR223015 | Ghrh (Myc-DDK-tagged) - Mouse growth hormone releasing hormone (Ghrh) |
CNY 1,200.00 |
|
| MR223015L3 | Lenti ORF clone of Ghrh (Myc-DDK-tagged) - Mouse growth hormone releasing hormone (Ghrh) |
CNY 4,750.00 |
|
| MR223015L4 | Lenti ORF clone of Ghrh (mGFP-tagged) - Mouse growth hormone releasing hormone (Ghrh) |
CNY 4,750.00 |
