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Wnt9b (NM_011719) Mouse Tagged ORF Clone
CAT＃: MG222833
- TrueORF®
Wnt9b (tGFP-tagged) - Mouse wingless-type MMTV integration site 9B (Wnt9b), (10ug)
ORF Plasmid: DDK
"NM_011719" in other vectors (4)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | clf; clf1; wnt-14b; wnt-15; Wnt14b; Wnt15 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222833 representing NM_011719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCCCGCGCCCGCGCTGGCCCTGGCTGCGCTCTGCCTGCTGGTGCTGCCTGCCGCTGCCGCCGCCG CCGCCTACTTCGGCCTGACCGGTCGTGAGGTCCTGACACCCTTCCCAGGCCTGGGTACGGCAGCAGCCCC GGCACAGGCTGGTGCTCACCTGAAGCAGTGTGACCTACTGAAGCTGTCCAGGCGGCAGAAGCAGCTCTGC AGGCGGGAGCCCGGCCTGGCTGAGACCCTGAGGGATGCTGCACACCTGGGGCTGCTGGAATGTCAGTTCC AGTTCAGGCAGGAGCGCTGGAACTGCAGCCTGGAGGGGAGGACTGGCCTGCTCCAGAGAGGCTTTAAGGA GACGGCCTTCCTGTATGCAGTGTCTGCAGCTGCCCTCACGCATGCACTGGCCAGGGCCTGCAGTGCTGGG CGCATGGAGCGCTGTACTTGTGACGACTCCCCAGGCCTGGAGAGCCGGCAGGCCTGGCAGTGGGGTGTGT GTGGTGACAATCTGAAGTACAGCACCAAGTTCCTCAGCAACTTCCTGGGGCCCAAGAGAGGAAGCAAGGA CCTGAGGGCGAGGGCTGACGCCCACAACACCCACGTGGGCATCAAGGCTGTGAAGAGCGGCCTGAGAACA ACCTGCAAGTGCCATGGTGTGTCAGGCTCCTGTGCTGTTCGTACCTGTTGGAAGCAGCTCTCCCCGTTTC GCGAGACCGGCCAGGTGCTGAAGCTACGCTATGACACGGCTGTCAAGGTGTCCAGTGCCACCAACGAGGC CTTGGGTCGTCTGGAGCTATGGGCCCCCGCTAAGCCAGGTGGTCCCGCCAAGGGCCTAGCCCCTCGTCCC GGGGACCTGGTCTACATGGAAGATTCTCCCAGCTTCTGCCGGCCCAGCAAGTACTCTCCGGGCACGGCAG GCAGGGTGTGTTCTCGAGACTCCAGTTGCAGCAGCCTATGCTGTGGGCGAGGCTACGACACCCAGAGCCG CATGGTGGTTTTCTCCTGCCACTGTCAGGTGCAGTGGTGCTGCTACGTGGAGTGCCAGCAGTGTGCACAG CAGGAGCTCGTGTATACCTGCAAGCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222833 representing NM_011719
Red=Cloning site Green=Tags(s) MRPAPALALAALCLLVLPAAAAAAAYFGLTGREVLTPFPGLGTAAAPAQAGAHLKQCDLLKLSRRQKQLC RREPGLAETLRDAAHLGLLECQFQFRQERWNCSLEGRTGLLQRGFKETAFLYAVSAAALTHALARACSAG RMERCTCDDSPGLESRQAWQWGVCGDNLKYSTKFLSNFLGPKRGSKDLRARADAHNTHVGIKAVKSGLRT TCKCHGVSGSCAVRTCWKQLSPFRETGQVLKLRYDTAVKVSSATNEALGRLELWAPAKPGGPAKGLAPRP GDLVYMEDSPSFCRPSKYSPGTAGRVCSRDSSCSSLCCGRGYDTQSRMVVFSCHCQVQWCCYVECQQCAQ QELVYTCKR TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011719 |
| ORF Size | 1077 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011719.4, NP_035849.3 |
| RefSeq Size | 4519 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 22412 |
| UniProt ID | O35468 |
| Gene Summary | Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:22461561, PubMed:16054034, PubMed:17537789). Required for normal embryonic kidney development, and for normal development of the urogenital tract, including uterus and part of the oviduct and the upper vagina in females, and epididymis and vas deferens in males (PubMed:16054034). Activates a signaling cascade in the metanephric mesenchyme that induces tubulogenesis (PubMed:16054034, PubMed:17537789). Acts upstream of WNT4 in the signaling pathways that mediate development of kidney tubules and the Muellerian ducts (PubMed:16054034). Plays a role in cranofacial development and is required for normal fusion of the palate during embryonic development (PubMed:16054034, PubMed:22461561, PubMed:25257647).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209649 | Wnt9b (untagged) - Mouse wingless-type MMTV integration site 9B (Wnt9b), (10ug) |
CNY 5,488.00 |
|
| MR222833 | Wnt9b (Myc-DDK-tagged) - Mouse wingless-type MMTV integration site 9B (Wnt9b) |
CNY 5,488.00 |
|
| MR222833L3 | Lenti ORF clone of Wnt9b (Myc-DDK-tagged) - Mouse wingless-type MMTV integration site 9B (Wnt9b) |
CNY 5,890.00 |
|
| MR222833L4 | Lenti ORF clone of Wnt9b (mGFP-tagged) - Mouse wingless-type MMTV integration site 9B (Wnt9b) |
CNY 5,890.00 |
