Ms4a2 (NM_013516) Mouse Tagged ORF Clone

CAT#: MG221668

  • TrueORF®

Ms4a2 (tGFP-tagged) - Mouse membrane-spanning 4-domains subfamily A member 2 (Ms4a2), (10ug)

ORF Plasmid: DDK tGFP



  "NM_013516" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Fce1b; Fcer1; Fcer1b; fcERI; FcR; FcRB; Fcrbe; Fcrbeta; Ms4a1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG221668 representing NM_013516
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACAGAAAATAGGAGCAGAGCAGATCTTGCTCTCCCAAATCCACAAGAATCCTCCAGTGCACCTG
ACATTGAACTCTTGGAAGCATCTCCTGCCAAAGCAGCCCCACCAAAGCAGACATGGCGGACATTTTTGAA
GAAAGAGTTGGAGTTCCTGGGAGCAACACAAATTCTGGTTGGTTTGATATGCCTTTGTTTTGGAACAATT
GTCTGCTCCGTACTCTATGTTTCAGACTTTGATGAAGAAGTGCTTTTACTTTATAAACTAGGCTATCCAT
TCTGGGGTGCAGTGCTGTTTGTTTTGTCTGGATTTTTGTCAATTATCTCCGAAAGAAAAAACACATTGTA
TCTGGTGAGAGGCAGCCTGGGAGCAAACATTGTCAGTAGCATCGCTGCAGGGACGGGGATCGCCATGCTG
ATCCTCAATCTGACCAATAACTTCGCTTATATGAACAACTGCAAGAATGTAACCGAAGACGACGGCTGCT
TTGTGGCTTCTTTCACCACAGAACTGGTGTTGATGATGCTGTTTCTCACCATCCTGGCCTTTTGCAGTGC
TGTGTTGTTCACTATCTATAGGATTGGACAAGAGTTAGAAAGTAAAAAGGTCCCAGATGATCGTCTTTAT
GAAGAATTAAATGTGTATTCACCAATTTACAGTGAGTTGGAAGACAAAGGGGAAACATCTTCTCCAGTTG
ATTCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG221668 representing NM_013516
Red=Cloning site Green=Tags(s)

MDTENRSRADLALPNPQESSSAPDIELLEASPAKAAPPKQTWRTFLKKELEFLGATQILVGLICLCFGTI
VCSVLYVSDFDEEVLLLYKLGYPFWGAVLFVLSGFLSIISERKNTLYLVRGSLGANIVSSIAAGTGIAML
ILNLTNNFAYMNNCKNVTEDDGCFVASFTTELVLMMLFLTILAFCSAVLFTIYRIGQELESKKVPDDRLY
EELNVYSPIYSELEDKGETSSPVDS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013516
ORF Size 2756 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013516.2, NP_038544.1
RefSeq ORF 708 bp
Locus ID 14126
UniProt ID P20490
Gene Summary This gene encodes a member of the membrane-spanning 4A family. The encoded protein is the beta subunit of the high affinity IgE receptor and is localized to the membrane. The encoded protein is required for full activation of mast cells, including the release of histamine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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