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Rims1 (NM_183018) Mouse Tagged ORF Clone
CAT＃: MG221626
- TrueORF®
Rims1 (tGFP-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_183018" in other vectors (4)
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CNY 15,770.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C030033M19Rik; mKIAA0340; Rab3ip1; Rim; RIM1; RIM1a; RIM1alpha; Serg1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221626 representing NM_183018
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCGGCCGTGGGGCCCCGAGGTCCTCGCCCACCCACGGTGCCTCCCCCTATGCAAGAACTGCCCG ACCTGAGCCACCTGACCGAGGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAGGA GGAAAAAGAAGAGGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAACACCA AGAAATGCTGAAAGCCAGCCCCATCAACCACCACTGAACATTTTCAGATGTGTCTGTGTTCCCAGAAAGC CAAGCAGCGAAGAGGGAGGCCCAGACAGAAACTGGAGATTGCATCAACAGTTTGAAAGCTACAAGGAACA AGTGAGAAAAATCGGAGAGGAAGCCAGGCGTTACCAGGGCGAGCACAAAGATGATGCCCCGACGTGTGGA ATCTGTCATAAGACAAAGTTTGCTGATGGATGTGGCCATCTCTGCTCCTATTGTCGCACCAAGTTCTGTG CACGCTGTGGAGGCCGAGTGTCTCTGCGATCGAACAATGAGGACAAAGTGGTTATGTGGGTATGCAATTT ATGTCGAAAGCAACAAGAGATATTAACGAAATCTGGAGCGTGGTTCTTCGGAAGTGGTCCTCAGCAGCCC AGTCAAGATGGGACTCTGAGTGACACGGCTACAGGTGCTGGATCTGAGGTGCCAAGAGAAAAGAAAGCTC GGCTCCAAGAGCGATCAAGGTCTCAGACGCCCTTGAGTACAGCAGCTGTCTCTTCCCAAGACACTGCTTC CCATGGTGCACCACTGGACAGGAACAAAGGGGCGGAGCCCTCACAGCAAGCCTTGGGTCCTGAACAGAAG CAGGCATCAAGGTCAAGAAGTGAGCCACCAAGGGAAAGGAAGAAGGCTCCAGGGCTTTCAGAGCAGAATG GCAAGGGAGGCCAGAAGAGTGAGCGCAAACGTGTCCCCAAGTCTGTGGTGCAACCCGGGGAAGGGACCGC GGATGAACGGGAGAGGAAGGAGAGGCGGGAAACCCGCAGGCTGGAGAAAGGGCGCTCTCAGGACTACCCG GACCGGCTCGAGAAACGCGAGGATGGCAGGGTGGCTGAAGACGAAAAGCAGAGGAAGGAGGAGGAGGGCG TGTCCACGCCGGAGTACACGAGCTGCGAGGACGTGGAGCTGGAGAGCGAGAGCGTGAGCGAGAAAGGTGA CTTGGACTACTGGTTGGATCCTGCCACGTGGCATAGCAGGGAAACGTCGCCTATTAGTTCGCATCCTGTA ACGTGGCAGCCATCTAAAGAGGGGGACCGATTAATCGGCCGTGTTATTCTTAACAAAAGAACAACCATGC CCAAAGAATCAGGTGCATTATTGGGTCTCAAGGTGGTTGGAGGAAAAATGACGGACTTAGGGCGTCTTGG TGCTTTCATCACCAAAGTAAAGAAGGGTAGCCTGGCAGATGTTGTCGGACACCTAAGAGCAGGGGATGAA GTTCTAGAGTGGAATGGTAAACCCCTGCCGGGAGCAACAAACGAAGAAGTTTACAACATTATTTTAGAAT CAAAATCAGAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGGTGACATCCCCAGGATCCCTGAGAG TTCCCACCCTCCTCTGGAGTCCAGTTCAAGTTCCTTTGAATCTCAGAAGATGGAAAGGCCTTCCATTTCT GTTATTTCTCCAACCAGCCCTGGAGCTCTGAAAGATGCCCCACAAGTCTTACCAGGGCAACTCTCAGTGA AGCTGTGGTATGATAAGGTGGGGCACCAGCTGATTGTAAATGTTCTACAAGCAACAGATCTACCCCCTAG AGTAGATGGGCGTCCCAGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGCGACAAGAGTAAA AGAAGAACCAAAACGGTAAAGAAACTTCTAGAGCCAAAATGGAATCAAACATTTGTCTACTCACACGTAC ATCGTCGAGATTTTCGAGAGCGAATGTTAGAAATTACTGTGTGGGACCAGCCAAGAGTACAGGACGAGGA GAGTGAATTTCTAGGAGAGATCCTCATAGAATTGGAAACAGCACTTTTAGATGATGAGCCCCATTGGTAT AAACTGCAGACACATGATGAATCTTCACTACCTCTGCCTCAGCCATCACCGTTCATGCCCAGGCGGCATA TCCACGGAGAAAGCTCCAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTA TGAGGTTGATGATGGTATTGGCGTAGTGCCTCCAGTGGGTTATAGAGCTAGTGCTAGAGAGAGTAAAGCC ACCACGTTAACAGTGCCAGAGCAGCAAAGAACCACACATCACCGCTCACGTTCCGTGTCTCCTCATCGCG GCGATGATCAGGGAAGGCCTCGTTCACGTTTACCAAATGTGCCATTACAGAGGAGCTTAGATGAAATCCA TCCAACACGAAGGTCACGTTCTCCAACCCGACACCATGATGCCTCCCGAAGCCTGGCCGATCACAGATCA CGACATGCGGAAAGTCAATATTCGTCAGAGCCAGACAGTGAGCTTCTCATGCTGCCCAGAGCAAAACGAG GACGAAGTGCAGAATGCCTACACATGACCAGTGAACTGCAGCCCTCTCTTGACAGGGCTAGGAGTGCTAG TACCAACTGCTTGAGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGCACTCCAGAAAGTCTGAA AGATCTAGCATCCAAAAACAGTCTAGGAAAGGCACAGCCTCTGATGCAGACAGGGTTCTCCCACCATGCC TTTCTAGAAGGGGATACGCAATCCCAAGAGCAACCGATCAACCGGTCATTAGGGGAAAGCATACCACTCG CTCCCGGTCGAGCGAGCACTCTAGTATCAGAACCCTGTGTTCTATGCACCACCTTGCCCCCGGAGGGTCA GCGCCGCCTTCTCCGCTTCTGACAAGAACGCACCGACAAGGAAGCCCCACCCAGTCACCTCCAGCTGACA CATCCTTTGGCAGTCGCCGCGGAAGACAGCTCCCACAGGTGCCGGTCCGAAGCGGCAGTATAGAACAAGC GAGCTTAGTAGTGGAGGAACGAACGAGACAGATGAAAATGAAGGTACACCGATTTAAGCAGACAACAGGG TCTGGGTCTAGTCAAGAACTTGACCACGAGCAATACTCCAAGTACAACATACATAAAGATCAGTACAGAA GCTGTGATAACGCGTCCGCCAAGTCATCAGATAGTGATGTCAGTGATGTGTCCGCCATTTCCAGAGCCAG CAGCACCTCACGCCTCAGCAGCACAAGCTTTATGTCAGAGCAGTCTGAGCGCCCCAGGGGCAGGATCAGT TCATTTACCCCCAAAATGCAAGGCAGACGGATGGGGACTTCAGGAAGAGCCATCATCAAGAGCACCAGTG TAAGTGGAGAGATATATACACTGGAGCACAATGACGGCAGCCAGTCCGACACGGCCGTGGGTACCGTTGG AGCTGGTGGAAAGAAGCGGAGGTCCAGCCTGAGTGCCAAAGTGGTAGCCATTGTGTCTCGAAGAAGCAGA AGCACATCACAGCTCAGCCAGACAGAGTCCGGCCACAAGAAGTTAAAAAGCACCATTCAGAGGAGTACGG AAACGGGAATGGCGGCTGAAATGCGGAAGATGGTGAGACAGCCCAGCCGGGAGTCCACGGATGGCAGCAT CAACAGTTATAGCTCAGAAGGAAACTTAATATTTCCTGGAGTTCGCGTGGGACCTGACAGTCAGTTCAGT GATTTCCTTGATGGACTGGGGCCAGCCCAGCTTGTCGGCCGCCAAACCCTAGCCACCCCAGCCATGGGCG ATATCCAAATCGGGATGGAGGATAAGAAGGGTCAGTTGGAGGTTGAGGTTATCAGAGCCCGGAGCCTTAC ACAAAAACCTGGCTCCAAATCTACACCTGCTCCCTATGTGAAAGTTTATCTTTTGGAAAATGGAGCCTGT ATTGCCAAAAAGAAGACAAGAATTGCACGGAAAACTCTCGATCCCTTGTATCAGCAGTCCCTGGTTTTTG ATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGTGACTATGGAAGAATGGACCACAAATG CTTTATGGGTGTGGCTCAAATCTTGTTGGAAGAACTTGATCTGTCCAGCATGGTGATTGGATGGTATAAA TTGTTCCCTCCATCCTCCCTGGTGGATCCCACACTCACTCCCCTGACCCGCCGGGCTTCCCAATCATCAC TGGAAAGTTCGTCTGGGCCTCCCTGCATCCGGTCA ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221626 representing NM_183018
Red=Cloning site Green=Tags(s) MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTP RNAESQPHQPPLNIFRCVCVPRKPSSEEGGPDRNWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCG ICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQP SQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTASHGAPLDRNKGAEPSQQALGPEQK QASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGTADERERKERRETRRLEKGRSQDYP DRLEKREDGRVAEDEKQRKEEEGVSTPEYTSCEDVELESESVSEKGDLDYWLDPATWHSRETSPISSHPV TWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDE VLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSIS VISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSK RRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWY KLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKA TTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSLADHRS RHAESQYSSEPDSELLMLPRAKRGRSAECLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKSE RSSIQKQSRKGTASDADRVLPPCLSRRGYAIPRATDQPVIRGKHTTRSRSSEHSSIRTLCSMHHLAPGGS APPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTG SGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRIS SFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLEHNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSR STSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFS DFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGAC IAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYK LFPPSSLVDPTLTPLTRRASQSSLESSSGPPCIRS TRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183018 |
| ORF Size | 4305 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183018.3 |
| RefSeq Size | 4308 bp |
| RefSeq ORF | 4308 bp |
| Locus ID | 116837 |
| UniProt ID | Q99NE5 |
| Gene Summary | Rab effector involved in exocytosis (PubMed:11797009). May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity (PubMed:11797009). Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224456 | Rims1 (untagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 2, (10ug) |
CNY 18,430.00 |
|
| MR221626 | Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 2 |
CNY 14,350.00 |
|
| MR221626L3 | Lenti ORF clone of Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 2 |
CNY 16,250.00 |
|
| MR221626L4 | Lenti ORF clone of Rims1 (mGFP-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 2 |
CNY 16,250.00 |
