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Pigt (NM_133779) Mouse Tagged ORF Clone
CAT＃: MG220624
- TrueORF®
Pigt (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis class T (Pigt), (10ug)
ORF Plasmid: DDK
"NM_133779" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2510012P17Rik; 4930534E15Rik; CGI-06; NDAP; Ndap7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220624 representing NM_133779
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCCATGCCGCTCGGTTTGCCGTTGCGGTTACTGGTGCTGTTGCTGGTGGGACGGGGCTGCT GTGGCTGCGCGGAGGGCCCACGCGACAGCCTGCGAGAAGAGCTCGTTATCACTCCACTGCCTTCCGGCGA CGTGGCCGCCACATTCCAGTTCCGCACGCGTTGGGATTCGGATCTGCAGCGGGAAGGAGTGTCCCATTAC AGGCTCTTCCCTAAAGCCCTGGGACAGCTGATCTCCAAGTACTCCCTGCGGGAGCTCCACCTGTCGTTCA CGCAAGGCTTTTGGAGGACGCGATACTGGGGGCCACCCTTCCTGCAGGCTCCGTCAGGTGCAGAGCTCTG GGTCTGGTTCCAAGACACTGTCACCGATGTGGATAAGTCCTGGAGGGAGCTCAGCAATGTCCTCTCGGGG ATCTTCTGTGCATCCCTCAACTTCATCGACGCCACCAATACCGTCACTCCCACCGCCTCCTTCAAACCTC TGGGACTGGCCAATGACACTGACGACTACTTCCTGCGCTACGCTGTGCTGCCTCGGGAGGTTGTCTGCAC TGAGAACCTCACGCCATGGAAGAAGCTCTTGCCCTGCAGTTCCAAGGCCGGTCTCTCAGTGCTATTGAAA GCAGACCGCTTGTTCCACACCAGTTACCACTCCCAGGCAGTGCACATCCGGCCTATCTGCAGAAATGCTC ACTGTACCAGCATCTCCTGGGAGCTGAGGCAGACCCTTTCGGTTGTCTTTGATGCCTTCATCACAGGACA GGGGAAGAAAGACTGGTCCCTCTTCCGCATGTTCTCCCGGACTCTCACAGAGGCCTGTCCATTGGCGTCT CAGAGCCTAGTTTATGTGGACATCACAGGCTACAGCCAGGATAATGAAACACTGGAGGTGAGCCCTCCCC CAACTTCCACATACCAGGATGTCATTTTGGGCACCAGGAAGACCTATGCCGTCTATGACTTGTTTGACAC AGCCATGATCAATAACTCTCGAAACCTCAACATCCAGCTCAAATGGAAGAGACCCCCAGATAATGAGGCC CTACCCGTGCCCTTCCTGCATGCGCAGCGGTACGTGAGTGGCTATGGACTGCAGAAGGGAGAGCTGAGTA CTCTGCTGTACAACTCCCATCCTTATCGGGCCTTCCCCGTGCTGCTGCTGGATGTCGTGCCCTGGTACCT GCGGCTGTATGTGCACACGCTCACCATTACCTCCAAGGGCAAGGAGAATAAACCAAGTTACATCCACTAC CAACCTGCTCAGGACAGGCAGCAGCCCCACCTCCTGGAGATGCTCATTCAGCTGCCAGCCAACTCCGTCA CCAAGGTCTCCATCCAGTTTGAACGAGCCCTGCTCAAGTGGACAGAGTACACACCAGACCCCAACCACGG CTTCTATGTCAGCCCATCTGTCCTCAGCGCCCTTGTGCCCAGTGTGGTGGCAGCCAAACCAGTGGACTGG GAAGGGAGCCCTCTCTTCAACACCTTGTTCCCGGTGTCTGATGGCTCCAGCTACTTTGTCCGACTCTACA CAGAGCCCTTGCTAGTGAACCTGCCCACCCCGGACTTCAGCATGCCCTACAATGTGATCTGCCTTACGTG CACGGTGGTGGCCGTGTGCTATGGCTCCTTCTACAATCTCCTCACCCGAACCTTTCACATCGAAGAGCCC AAGTCGGGCGGCCTGGCCAAGCGGCTGGCTAACCTCATCCGCCGTGCCCGTGGTGTGCCCCCTCTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220624 representing NM_133779
Red=Cloning site Green=Tags(s) MAAAMPLGLPLRLLVLLLVGRGCCGCAEGPRDSLREELVITPLPSGDVAATFQFRTRWDSDLQREGVSHY RLFPKALGQLISKYSLRELHLSFTQGFWRTRYWGPPFLQAPSGAELWVWFQDTVTDVDKSWRELSNVLSG IFCASLNFIDATNTVTPTASFKPLGLANDTDDYFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLSVLLK ADRLFHTSYHSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMFSRTLTEACPLAS QSLVYVDITGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDTAMINNSRNLNIQLKWKRPPDNEA LPVPFLHAQRYVSGYGLQKGELSTLLYNSHPYRAFPVLLLDVVPWYLRLYVHTLTITSKGKENKPSYIHY QPAQDRQQPHLLEMLIQLPANSVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVAAKPVDW EGSPLFNTLFPVSDGSSYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEEP KSGGLAKRLANLIRRARGVPPL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133779 |
| ORF Size | 1746 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133779.2, NP_598540.2 |
| RefSeq Size | 2574 bp |
| RefSeq ORF | 1749 bp |
| Locus ID | 78928 |
| UniProt ID | Q8BXQ2 |
| Gene Summary | Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219322 | Pigt (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (Pigt), (10ug) |
CNY 7,410.00 |
|
| MR220624 | Pigt (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (Pigt) |
CNY 4,336.00 |
|
| MR220624L3 | Lenti ORF clone of Pigt (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (Pigt) |
CNY 6,460.00 |
|
| MR220624L4 | Lenti ORF clone of Pigt (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (Pigt) |
CNY 6,460.00 |
