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Dusp12 (NM_023173) Mouse Tagged ORF Clone
CAT＃: MG220464
- TrueORF®
Dusp12 (tGFP-tagged) - Mouse dual specificity phosphatase 12 (Dusp12), (10ug)
ORF Plasmid: DDK
"NM_023173" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1190004O14Rik; AA027408; AW049275; ENSMUSG00000045460; ESTM36; LMW-DSP4; mVH1; T-DSP4; VH1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220464 representing NM_023173
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGAAGCGCAGGGTTCTAACCATGGCTGCGAGCGCCAGGCTCCGACTGCCAGTCCCGCCAGCTCGG CAGGGCACGCCGTGGAAGTGCGGCCCGGGCTGTATCTGGGTGGGGCAGCGGCAGTGGCGGAACCGGGCCA TCTGAGGGAGGCGGGCATCACCGCCGTGCTGACGGTGGACTCTGAACCGGCTTTCCCGGCTGGGGCTGGG TTCGAAGGTCTCCGGAGCCTCTTCGTGCCGGCGCTGGACAAACCCGAGACCGACCTGCTCAGCCACCTGG ATCGCTGCGTGGCCTTCATCGGCCAGGCTCGCTCCGAAGGCCGCGCGGTGTTGGTGCACTGTCATGCAGG AGTCAGTCGCAGTGTTGCTGTAGTGATGGCTTTTATAATGAAGACCGACCAGCTTACCTTTGAAAAAGCC TACGACATCCTCCGGACGGTCAAGCCAGAGGCTAAAGTGAATGAGGGGTTTGAATGGCAACTGAAACTGT ATGAGGCAATGGGATACGAAGTCGATACGTCCAGTGCCTTTTACAAGCAGTACCGTTTACAAAAGGTGAC TGAGAAGTGTCCAAAACTTTGGAATTTACCTCAGGAACTCTTCGCTGTTGACCCAACTACCATTTCACAG GGATTAAAAGATGACATTCTCTACAAATGCAGAAAATGCAGGCGGTCTTTATTTAGACATTCTAGTATTT TGGGTCATAGTGAAGGAAGTGGGCCAATAGCCTTTGCTCACAAGAGAACGGCGCCATCTTCTGTACTTAC CACAGGGAGTCAGGCTCAGTGCACGTCTTACTTCATTGAGCCTGTGCAGTGGATGGAATCTACTCTGTTG GGCGTTATGGATGGACAGCTTCTTTGCCCAAAATGCAGTGCCAAGTTGGGTTCCTTTAACTGGTATGGTG AACAGTGCTCCTGTGGTCGATGGATAACCCCTGCTTTTCAAATACACAAGAACAGAGTGGATGAAATGAA AATGTTGCCGGTGCTGGGATCACAGACAAAGAAGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220464 representing NM_023173
Red=Cloning site Green=Tags(s) MLEAQGSNHGCERQAPTASPASSAGHAVEVRPGLYLGGAAAVAEPGHLREAGITAVLTVDSEPAFPAGAG FEGLRSLFVPALDKPETDLLSHLDRCVAFIGQARSEGRAVLVHCHAGVSRSVAVVMAFIMKTDQLTFEKA YDILRTVKPEAKVNEGFEWQLKLYEAMGYEVDTSSAFYKQYRLQKVTEKCPKLWNLPQELFAVDPTTISQ GLKDDILYKCRKCRRSLFRHSSILGHSEGSGPIAFAHKRTAPSSVLTTGSQAQCTSYFIEPVQWMESTLL GVMDGQLLCPKCSAKLGSFNWYGEQCSCGRWITPAFQIHKNRVDEMKMLPVLGSQTKKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023173 |
| ORF Size | 1017 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023173.2, NP_075662.2 |
| RefSeq Size | 1413 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 80915 |
| UniProt ID | Q9D0T2 |
| Gene Summary | Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro). Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211938 | Dusp12 (untagged) - Mouse dual specificity phosphatase 12 (Dusp12), (10ug) |
CNY 3,990.00 |
|
| MR220464 | Dusp12 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 12 (Dusp12) |
CNY 2,850.00 |
|
| MR220464L3 | Lenti ORF clone of Dusp12 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 12 (Dusp12) |
CNY 4,750.00 |
|
| MR220464L4 | Lenti ORF clone of Dusp12 (mGFP-tagged) - Mouse dual specificity phosphatase 12 (Dusp12) |
CNY 4,750.00 |
