United States
Germany
Japan
United Kingdom
China
Pde2a (NM_001143849) Mouse Tagged ORF Clone
CAT＃: MG219052
- TrueORF®
Pde2a (tGFP-tagged) - Mouse phosphodiesterase 2A cGMP-stimulated (Pde2a) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001143849" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CGS-PDE; cGSPDE |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219052 representing NM_001143849
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCTGGTGTTGCACCACATCCTCATCGCTGTTGTCCAATTCCTCAGGCGGGGCCAGCAGGTCTTCC TCAAGCCGGACGAGCCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGATGCTTTGCTGAGCCTAGGCGC CGTTATCGACATTGCTGGCCTGCGACAGGCTGCCAGGGATGCCCTCTCAGCCGTGCTCCCCAAAGTGGAG ACTGTCTACACCTACCTGCTAGATGGGGAGTCCAGACTGGTGTGTGAGGACCCCCCTCATGAGCTGCCAC AGGAAGGAAAAATTCGAGAAGCTGTGATCTCTCAGAAGCGGCTGAGTTGCAATGGGCTGGGACCTTCGGA CCTACTGGGAAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCTCCTGACATGCAAGTGCTGGTCATACCC CTGCTGGACAAGGAGACTGGAAGTGTGGCAGCTGTCATCTTGGTGCACTGTGGCCAGCTGAGTGACAGTG AGGAACAGAGCTTGCAGGTGGTAGAGAAGCACGCTCTGGTAGCCCTGCGGAGGGTGCAGGCCCTGCAGCA GCGCAGGCCTGAAGCTGTTCAGAACACGTCAGTGGATGCCTCTGAAGATCAAAAGGATGAGAAGGGGTAC ACCGACCATGACCGAAAGATCCTGCAACTGTGTGGGGAACTCTTTGACTTGGATGCTACTTCTCTGCAGC TCAAAGTCCTTCAATATCTGCAGCAGGAGACACAGGCCACTCACTGCTGCCTCCTGCTGGTGTCGGAGGA CAATCTACAGCTTTCCTGCAAGGTCATTGGAGACAAAGTGCTGGGAGAAGAGGTCAGCTTTCCATTGACC ATGGGACGTCTGGGGCAGGTGGTGGAAGACAAGCAGTGTATCCAGTTGAAGGACCTAACCTCTGACGATG TGCAACAGCTGCAAAACATGTTGGGTTGTGAGCTGCAGGCTATGCTGTGTGTCCCTGTCATCAGCCGAGC CACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAACAAGCTTGGAGGAGATTTCTTCACAGATGAGGAT GAACACGTGATCCAACACTGCTTCCACTACACAGGCACGGTGCTCACCAGCACCTTGGCCTTCCAGAAGG AGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCAAAGAACCTCTTCACCCACCTGGATGA TGTCTCTGTCCTGCTACAGGAGATCATCACGGAAGCCAGAAACCTCAGCAACGCAGAGATCTGCTCGGTG TTCCTGCTGGATCAGAACGAGCTGGTGGCCAAGGTGTTCGATGGTGGCGTTGTGGACGATGAGAGTTATG AGATCCGCATCCCGGCGGACCAAGGCATCGCGGGCCACGTGGCGACTACGGGCCAGATCCTGAACATCCC AGATGCATATGCCCATCCGCTTTTCTATCGCGGCGTAGATGACAGCACTGGCTTCCGCACGCGCAACATT CTCTGCTTCCCTATCAAGAACGAGAACCAGGAGGTCATTGGTGTGGCTGAGCTAGTGAACAAGATCAATG GGCCATGGTTCAGCAAGTTTGATGAGGACCTGGCCACAGCCTTCTCCATCTACTGTGGCATCAGCATCGC CCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCAATACCGCAGCCACCTGGCCAATGAGATGATGATG TATCATATGAAGGTCTCTGATGATGAATACACCAAGCTTCTCCACGATGGCATCCAACCTGTGGCCGCCA TTGACTCCAACTTTGCCAACTTTACCTACACGCCTCGGTCTCTGCCTGAGGACGACACTTCTATGGCCAT CCTGAGCATGCTGCAAGACATGAACTTCATCAATAACTACAAAATTGACTGCCCAACTCTGGCCCGATTC TGCCTGATGGTGAAGAAAGGCTACCGGGATCCACCCTACCACAACTGGATGCACGCCTTCTCTGTCTCTC ATTTTTGCTACCTGCTCTACAAGAATCTGGAGCTCTCCAACTACCTCGAGGACATCGAGATCTTTGCATT GTTTATTTCCTGCATGTGTCATGACCTGGACCACAGAGGCACAAACAACTCCTTCCAGGTGGCCTCGAAA TCTGTGCTGGCCGCACTCTACAGCTCAGAGGGCTCTGTCATGGAGAGGCACCACTTTGCTCAAGCCATTG CTATCCTCAACACCCACGGCTGCAATATCTTTGACCACTTCTCTCGGAAGGACTATCAGCGCATGCTGGA CCTGATGAGGGACATCATCTTGGCTACAGACCTGGCACACCACCTCCGCATCTTCAAGGACCTGCAGAAG ATGGCTGAAGTGGGTTATGACCGAAACAACAGGCAACACCACAGGCTTCTTCTGTGCCTCCTCATGACCT CCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGACCACCAGAAAGATTGCAGAGCTGATCTACAAAGA GTTCTTCTCCCAGGGAGACTTGGAGAAGGCCATGGGCAACCGACCGATGGAGATGATGGACCGTGAGAAG GCCTACATCCCTGAGCTTCAGATCAGCTTTATGGAGCACATTGCCATGCCTATCTACAAGCTTTTACAAG ACCTGTTCCCCAAGGCGGCAGAGCTGTATGAACGTGTGGCCTCCAACCGTGAGCACTGGACCAAGGTGTC CCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAACTCGCTGGATTTCCTGGACGAGGAATACGAGGTC CCCGATTTGGACGGCACCAGAGCTCCTGTCAATGGCTGCTGCAGCCTCGAGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219052 representing NM_001143849
Red=Cloning site Green=Tags(s) MVLVLHHILIAVVQFLRRGQQVFLKPDEPPPQPCADSLQDALLSLGAVIDIAGLRQAARDALSAVLPKVE TVYTYLLDGESRLVCEDPPHELPQEGKIREAVISQKRLSCNGLGPSDLLGKPLARLVAPLAPDMQVLVIP LLDKETGSVAAVILVHCGQLSDSEEQSLQVVEKHALVALRRVQALQQRRPEAVQNTSVDASEDQKDEKGY TDHDRKILQLCGELFDLDATSLQLKVLQYLQQETQATHCCLLLVSEDNLQLSCKVIGDKVLGEEVSFPLT MGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELQAMLCVPVISRATDQVVALACAFNKLGGDFFTDED EHVIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSV FLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNI LCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMM YHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARF CLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASK SVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQK MAEVGYDRNNRQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREK AYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEV PDLDGTRAPVNGCCSLEG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001143849 |
| ORF Size | 2784 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001143849.2, NP_001137321.1 |
| RefSeq Size | 4067 bp |
| RefSeq ORF | 2787 bp |
| Locus ID | 207728 |
| UniProt ID | Q922S4 |
| Gene Summary | Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222657 | Pde2a (untagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3, (10ug) |
CNY 11,880.00 |
|
| MR219052 | Pde2a (Myc-DDK-tagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3 |
CNY 7,480.00 |
|
| MR219052L1 | Lenti ORF clone of Pde2a (Myc-DDK-tagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3 |
CNY 10,070.00 |
|
| MR219052L2 | Lenti ORF clone of Pde2a (mGFP-tagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3 |
CNY 9,880.00 |
|
| MR219052L3 | Lenti ORF clone of Pde2a (Myc-DDK-tagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3 |
CNY 10,070.00 |
|
| MR219052L4 | Lenti ORF clone of Pde2a (mGFP-tagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3 |
CNY 10,070.00 |
