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Pak6 (NM_001033254) Mouse Tagged ORF Clone
CAT＃: MG218274
- TrueORF®
Pak6 (tGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 6 (Pak6) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001033254" in other vectors (4)
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CNY 7,510.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4732456M09 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218274 representing NM_001033254
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCGCAAGAAAAAGAAGAAACGTCCTGAGATATCAGCCCCGCAGAACTTCCAGCATCGTGTCCACA CCTCCTTTGACCCCAAGGAAGGCAAGTTTGTGGGTCTGCCCCCACAATGGCAGAACATTCTGGATACTCT GAGGCGGCCCAAGCCTGTGGTGGATCCTTCTCGAATCACACGGGTGCAGTTGCAGCCCATGAAGACAGTC GTACGGGGCAGCTCAGTGCCCACGGAGGGCTACATCTCGGGGCTGCTCAACGACATTCAGAAGTTGTCAG TTATCAGCTCCAACACCCTGCGTGGACGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCT GGGAGATGATCAGTGGGCTGCTGACCCCGATATGTACCTCCAAAGCCCCCAGTCGGAGCACACTGATCCC CATGGCCTCTACCTCAGCTGCAATGGGGGCACACCAGCAGGTCACAGGCAGGTGCCCTGGCCAGAACCAC AGAGCCCGCAGGCCCTGCCCAATGGGATGGCTGCCAAGGCGCAGTCACTGGGCCCTGCTGAGTTCCAGGG TGCCTCACAACGCTGCCTGCAGCAGCTGGGTGCCTGCCTGCAGAGTTCCCCACCCGGTACCTCACTCCCT ATGGCCACAGGGAGGCGTGGGGTGAAGGTTGCCAAGCATAGCTCCGAAGAGGCCCGGCCACAGTCCTGCC TGGTGGGCTCGGCCATTGGCAGGCCAGGTGGAGAAGGCAGCCCTAGCCCTAAGAACCAGGAAAGCAGCCT CAAGCACCGGCTCTTCCGAAGCATGTTCCTGTCCACTCCTGCCACAGGCGCGGCAAGCAGCAGCAAGCCG GTCCCTCTGCCGCAGAACAAGCCCAATTCTGCTTTCCGACCACCACAAAAAGACTCCTCTTCAAACCTGG TAGCCAAGGCCCAGTCCTTGCCCTCAGAGCAGCCCATGGGGACCTTCAGTCCTCTGACCACCTCAGATAC CAGCAGCCCCCAGAAGTCCCTCCGCACAGTTCCAGCCGCTGGTCCACTTCCAGGCCGGTCTTCTCCAGCA GGCTCCCCCCGTACCCGGCATGCTCAGATCAGCACCAGCAATCTCTACCTGCCCCAGGACCCCACAGTGG CCAAGGGGGCCCTGGGTGGCGAAGACACGGGCATTGTGACCCATGAACAGTTCAAGGCTGCGCTCAGGAT GGTGGTGGACCAGGGTGACCCTCGGCTGCTGCTGGACAGCTACGTGAAGATTGGGGAGGGCTCCACGGGC ATTGTGTGTCTGGCCCGGGAGAAGCACTCAGGTCGCCAGGTAGCAGTCAAGATGATGGACCTCAGGAAGC AGCAACGCAGGGAGCTGCTCTTTAATGAGGTGGTGATCATGCGTGACTACCAGCACCTCAACGTGGTGGA GATGTACAAGAGTTACCTGGTAGGAGAGGAGCTGTGGGTGTTGATGGAGTTCCTGCAGGGCGGGGCCCTC ACAGACATCATCTCCCAAGTCAGACTGAATGAGGAGCAGATTGCCACTGTGTGTGAAGCTGTGCTTCAGG CCTTGGCTTACCTGCACGCCCAGGGTGTCATCCACCGAGATATCAAGAGTGATTCCATCCTGCTGACCCT TGATGGCAGGGTGAAGCTCTCAGACTTTGGGTTCTGTGCTCAGATCAGCAAAGATGTTCCCAAGAGAAAG TCCCTGGTAGGAACTCCATACTGGATGGCTCCTGAAGTGATCTCCAGATCCCTGTACGCTACTGAGGTGG ATATCTGGTCTCTGGGCATCATGGTGATTGAGATGGTGGATGGAGAACCCCCCTACTTCAGTGACTCCCC AGTGCAAGCCATGAAGAGGCTGCGGGACAGCGCCCCACCCAAGCTAAAGAACTCTTACAAGGTCTCTCCA GTGCTGCGAGACTTCCTGGATCGGATGCTGGTACGGGAACCCCAAGAGCGAGCCACGGCCCAGGAGCTCC TGGATCACCCGTTTCTGCTGCAGACGGGGCTGCCTGAGTGTCTGGTGCCTCTGATACAGCTCTACCGCAA GCAGACCTCCACCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218274 representing NM_001033254
Red=Cloning site Green=Tags(s) MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTV VRGSSVPTEGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDDQWAADPDMYLQSPQSEHTDP HGLYLSCNGGTPAGHRQVPWPEPQSPQALPNGMAAKAQSLGPAEFQGASQRCLQQLGACLQSSPPGTSLP MATGRRGVKVAKHSSEEARPQSCLVGSAIGRPGGEGSPSPKNQESSLKHRLFRSMFLSTPATGAASSSKP VPLPQNKPNSAFRPPQKDSSSNLVAKAQSLPSEQPMGTFSPLTTSDTSSPQKSLRTVPAAGPLPGRSSPA GSPRTRHAQISTSNLYLPQDPTVAKGALGGEDTGIVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTG IVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHLNVVEMYKSYLVGEELWVLMEFLQGGAL TDIISQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRK SLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSAPPKLKNSYKVSP VLRDFLDRMLVREPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033254 |
| ORF Size | 2046 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033254.3, NP_001028426.2 |
| RefSeq Size | 4137 bp |
| RefSeq ORF | 2049 bp |
| Locus ID | 214230 |
| UniProt ID | Q3ULB5 |
| Gene Summary | Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220453 | Pak6 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 6 (Pak6), transcript variant 1, (10ug) |
CNY 8,740.00 |
|
| MR218274 | Pak6 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 6 (Pak6), transcript variant 1 |
CNY 5,000.00 |
|
| MR218274L3 | Lenti ORF clone of Pak6 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 6 (Pak6), transcript variant 1 |
CNY 8,740.00 |
|
| MR218274L4 | Lenti ORF clone of Pak6 (mGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 6 (Pak6), transcript variant 1 |
CNY 8,740.00 |
