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Pan2 (NM_133992) Mouse Tagged ORF Clone
CAT＃: MG211796
- TrueORF®
Pan2 (tGFP-tagged) - Mouse ubiquitin specific peptidase 52 (Usp52)
ORF Plasmid: DDK
"NM_133992" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1200014O24Rik; AI047843; AW742773; mKIAA0710; Usp52 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211796 representing NM_133992
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTTGAGGGTCTGGATCCTGGACTGGCCGAATTTTCCCCAGCTATGCATTCTACCCTGGACCCAG TCCTGGATGCCCACCTGAATCCAAGTTTGCTACAGAATGTGGAGCTAGACCCAGAGGGAGTGGCCTTGGA GGCTCTTCCTGTCCAGGAGTCAGTGCACATAATGGAAGGTGTCTACTCTGAGTTGCACAGCGTGGTGGCT GAAGTGGGAGTGCCTGTGTCTGTCTCCCACTTTGATTTACATGAGGAGATGCTGTGGGTGGGGAGCCATG GGGGCCATGCCACCTCCTTCTTCGGCCCAGCTCTGGAGCGCTACTCATCCTTTCAGGTCAATGGTGGTGA CGACATTCGGCAAATCCAGAGCCTGGAGAACGGTATCCTCTTCCTCACCAAGAACAACCTCAAGTACATG GCCCGTGGAGGGCTCATTATATTTGACTATTTGCTGGATGAAAACGAGGATATGCACAGCGTCCTCCTGA CAGACAACAGCACTCTGCTCGTTGGGGGGCTGCAGAACCACGTACTGGAGATTGACCTGAACACTGTCCA GGAGACTCAGAAGTATGCAGTCGAGACACCCGGAGTCACCATCATGAGACAGACAAATCGTTTCTTCTTC TGTGGCCACACATCTGGCAAGGTTTCCCTGCGAGACCTCCGTAGTTTTAAAGTGGAGCATGAATTTGATG CCTTCTCAGGGAGTCTGTCAGATTTTGATGTTCATGGCAACCTGCTGGCTGCCTGCGGCTTCTCCAGTCG CCTTACCGGCCTGGCCTGTGACCGTTTCCTCAAAGTGTACGATCTGCGCATGATGCGTGCCATCACACCT CTTCAAGTGCACGTGGATCCGGCCTTCTTACGATTCATCCCCACATACACTTCCCGCCTTGCTATCATCT CCCAGTCAGGTCAATGCCAGTTTTGTGAACCCACAGGCCTGGCCAACCCAGCCGACATTTTCCATGTGAA TCCCGTGGGACCTTTGCTAATGACGTTTGATGTGTCAGCCAGCAAGCAGGCCCTGGCCTTTGGGGATTCT GAGGGCTGCGTGCATCTCTGGACTGATTCCCCTGAGCCGTCCTTCAACCCCTACTCCCGAGAGACTGAGT TTGCCCTGCCCTGTCTTGTGGACTCCTTGCCGCCTCTGGACTGGAGCCAGGATCTGCTGCCGCTTTCCCT CATTCCCGTCCCACTTACCACTGATGCACTGCTCTCTGACTGGCCTGCTGCCAACTCTGCTCCCGCTCCC AGGCGAGCACCACCTGTGGATGCAGAAATTCTTCGAACCATGAAGAAAGTGGGCTTCATTGGCTATGCTC CCAACCCTCGCACCAGGCTGCGCAACCAGATTCCCTATCGACTAAAGGAGTCAGACCATGAATTTGACAA CTTCAGCCAAGTCACGGAGTCACCGACAGGGCGAGAAGAGGAGCCTCTCCACACAGTTTCTAAGAAATAC CGGAAGGTAACCATCAAATATTCCAAGCTAGGCCTGGAAGACTTTGACTTCAAACACTACAATAAGACTC TGTTTGCTGGGTTAGAGCCTCACATCCCCAATGCCTACTGTAACTGCATGATCCAGGTGCTCTATTTCTT GGAGCCTGTTCGCTGTCTGATCCAGAACCACCTTTGCCAAAAGGAGTTCTGCCTAGCGTGCGAGCTCGGC TTCCTCTTCCACATGCTAGACCTCTCTCGTGGCGATCCTTGTCAGGGCAGTAATTTCCTCCGAGCTTTCC GGACCATCCCTGAGGCCTCAGCTCTGGGTCTCATCCTTGCTGACTCAGATGAGGCTTCAGGCAAGGGCAG TCTGGCCCGGCTCATCCAGAGGTGGAACCGCTTCATCCTCACTCAGCTACATCAGGATATGCAGGAGCTG GAAGTGCCCCAGGCTTATCGAGGTGCTGGAGGCAGTTTCTGCTCATCAGGAGACTCCATCATTGGGCAGC TGTTCAGTTGTGAGATGGAGAACTGCAGTCTTTGCCGCTGCGGCAGCGAGACTGTGCGGGCCTCGTCCAC CCTACTCTTCACACTCTCCTACCCTGAGGATAAAACCGGGAAGAACTATGACTTTGCTCAGGTGCTGAAG CGAAGTATCTGCCTGGAGCAGAACACACAGGCCTGGTGTGACAACTGTGAGAAGTACCAGCCCACAATTC AGACCAGAAACATCCGACATCTGCCAGATATTCTTGTCATTAACTGTGAAGTGAATAGCTCAAAAGAGGC TGATTTCTGGAGACTGCAAGCTGAGGTTGCCTTCAAAATAGCGGTGAAAAAGTACGGCGGGGAAATGAAG AGCAAAGAGTTTGCTTTAGCTGATCGGAAGGAACTGAGGAGTCCAGAGGGCTTTCTGTGTTCCTCCATCG AGGAGCTGAAGAATGTCTGGCTTCCATTTTCCATCCGCATGAAGATGACCAAGAACAAAGGACTGGATGT TTGCAACTGGGCTGACGAGCATGAGCTTAGCAGCCTGGGTGCCCCCTCACAGTGGGGTCCGGCCAGGGCA GAGGAGGAGCTTGGTGTCTATGTATATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGAACAG GCGGCAGCTTGGTGGCTCACATCAAAGTCGGTGAGACCTACCACCAGCGCAAGGAGGGCGTGACTCATCA GCAGTGGTATCTCTTCAATGACTTCCTTATTGAACCTATTGATAAGTATGAAGCCGTGCAGTTTGACATG AATTGGAAAGTACCTGCTATCCTGTATTATGTCAAAAGGAATCTCAATTCCAGATACAATCTGAATATCA AGAACCCCATTGAGGCCAGTGTGCTGCTGGCAGAAGCCTCGCTGGCACGGAAGCAACGGAAAACACACAC GACCTTTATTCCATTGATGCTGAATGAGATGCCACAGGTCGGGGACCTGGTGGGCCTGGATGCCGAGTTT GTCACTCTTAATGAGGAAGAAGCAGAGTTACGCAGTGATGGTACCAAGTCCACCATTAAGCCAAGCCAGA TGTCAGTAGCAAGGATCACCTGTGTTCGGGGCCAAGGGCCTAACGAGGGCATCCCCTTCATTGATGACTA CATCTCCACGCAGGAGCAGGTAGTAGATTACTTGACTCAGTACTCGGGGATAAAGCCAGGAGACCTTGAT GCCAAAATCTCCTCAAAGCACCTCACAACCCTCAAGTCTACCTACTTAAAGCTTCGCTTTCTCATTGATA TTGGAGTCAAGTTTGTGGGTCATGGTCTGCAGAAGGACTTCCGGGTCATCAACCTCATGGTGCCCAAGGA CCAAGTTCTTGACACAGTCTACCTGTTCCACATGCCCCGAAAACGAATGATTTCCCTACGATTCCTGGCC TGGTATTTTCTAGACCTGAAGATTCAAGGTGAGACCCATGACAGCATTGAGGATGCCCGCACAGCCCTTC AACTCTACCGGAAGTATCTGGAGCTGAGCAAGAACGGCACCGAGCCCGAGTCCTTCCACAAGGTGCTCAA GGGTCTCTATGAGAAGGGCCGAAAGATGGACTGGAAGGTGCCTGAGCCTGAAAGCCAGACAAGTCCCAAG AATGCAGCTGTCTTCTCAGTGCTGGCACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211796 representing NM_133992
Red=Cloning site Green=Tags(s) MNFEGLDPGLAEFSPAMHSTLDPVLDAHLNPSLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVA EVGVPVSVSHFDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGGDDIRQIQSLENGILFLTKNNLKYM ARGGLIIFDYLLDENEDMHSVLLTDNSTLLVGGLQNHVLEIDLNTVQETQKYAVETPGVTIMRQTNRFFF CGHTSGKVSLRDLRSFKVEHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAITP LQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLMTFDVSASKQALAFGDS EGCVHLWTDSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDLLPLSLIPVPLTTDALLSDWPAANSAPAP RRAPPVDAEILRTMKKVGFIGYAPNPRTRLRNQIPYRLKESDHEFDNFSQVTESPTGREEEPLHTVSKKY RKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACELG FLFHMLDLSRGDPCQGSNFLRAFRTIPEASALGLILADSDEASGKGSLARLIQRWNRFILTQLHQDMQEL EVPQAYRGAGGSFCSSGDSIIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPEDKTGKNYDFAQVLK RSICLEQNTQAWCDNCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRLQAEVAFKIAVKKYGGEMK SKEFALADRKELRSPEGFLCSSIEELKNVWLPFSIRMKMTKNKGLDVCNWADEHELSSLGAPSQWGPARA EEELGVYVYDLMATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKYEAVQFDM NWKVPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQVGDLVGLDAEF VTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLD AKISSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLA WYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPESQTSPK NAAVFSVLAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133992 |
| ORF Size | 3600 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133992.3 |
| RefSeq Size | 4440 bp |
| RefSeq ORF | 3603 bp |
| Locus ID | 103135 |
| UniProt ID | Q8BGF7 |
| Gene Summary | Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1 (PubMed:16284618). Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203510 | Pan2 (untagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2), (10ug) |
CNY 8,360.00 |
|
| MR211796 | Pan2 (Myc-DDK-tagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2) |
CNY 8,360.00 |
|
| MR211796L3 | Lenti ORF clone of Pan2 (Myc-DDK-tagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2) |
CNY 11,500.00 |
|
| MR211796L4 | Lenti ORF clone of Pan2 (mGFP-tagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2) |
CNY 11,500.00 |
