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Usp8 (NM_019729) Mouse Tagged ORF Clone
CAT＃: MG211601
- TrueORF®
Usp8 (tGFP-tagged) - Mouse ubiquitin specific peptidase 8 (Usp8)
ORF Plasmid: DDK
"NM_019729" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI574262; AW557536; mKIAA0055; Ubpy |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211601 representing NM_019729
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCTGTAGCTTCAGTTCCTAAAGAACTCTACCTCAGTTCTTCACTAAAAGACCTCAATAAGAAGA CCGAAGTTAAACCTGAGAAAACCAGCACCAAGAATTATATACACAGCGCGCAGAAGATCTTCAAGACAGC AGAAGAATGCAGACTAGATCGTGATGAGGAAAGGGCCTATGTGCTTTATATGAAATATGTGGCAGTTTAT AATCTTATCAAAAAGAGACCTGATTTCAAGCAACAGCAGGATTATTATCTTTCAATACTTGGACCTGCAA ACATCAAAAAAGCTATTGAAGAAGCTGAAAGACTCTCCGAAAGCCTTAAACTAAGATACGAAGAGGCTGA AGTTCGGAAACAACTTGAAGAAAAGGACAGACGGGAGGAAGAGCAGCTGCAGCAACAGAAAAGGCAGGAG ATGGGGAGAGAGGACAGCGGTGCGGCAGCCAAACGCTCTGTGGAAAATTTACTGGATTCCAAAACCAAAA CCCAAAGGATTAACGGCGAGAAGAGTGAAGGAGCTGCAGCTGCAGAGAGAGGAGCCATCACAGCAAAGGA ACTATATACGATGATGATGGATAAAAACACAAGCTTAATTATAATGGATGCTCGAAAAATACAGGATTAT CAGCATTCCTGTATCTTGGATTCTCTCAGTGTTCCTGAAGAAGCTATCAGTCCAGGAGTCACTGCTAGTT GGATTGAAGCAAACCTCTCAGATGATTCTAAAGACACATGGAAAAAGAGGGGGAGTGTGGACTATGTGGT CCTTCTCGACTGGTTTAGTTCAGCGAAAGATTTGCTGCTTGGGACCACTCTACGGAGTCTGAAAGATGCA CTTTTCAAGTGGGAAAGTAAAACTGTCCTGCGCCATGAGCCTTTGGTGTTGGAGGGCGGCTATGAAAACT GGCTGCTTTGCTACCCGCAGTTTACAACCAATGCTAAGGTCACTCCACCCCCTCGGAGCAGAGCTGAAGA GGTGTCTGTCTCATTGGATTTTACTTATCCCTCATTGGAAGAACCAGTTCCTTCCAAACTTCCTACCCAG ATGCCACCTCCTCCTATAGAAACAAATGAAAAGGCACTGTTGGTAACTGATCAAGATGAAAAGCTGAGAC TGTCAACCCAGCCAGCTCTAGCTGGACCTGGTGCGGCTCCCAGAGCTGAAGCCTCACCCATAATTCAGCC AGCGCCTGCTACAAAGAGTGTTCCACAGGTTGATCGTACGAAAAAACCGTCAGTCAAGTTGCCTGAAGAT CATAGAATAAAATCTGAAAATACAGATCAGAGTGGAAGAGTTCTTTCTGATCGATCCACCAAGCCAGTAT TTCCCTCTCCAACCACCATGTTAACAGATGAAGAAAAGGCTCGTATTCATCAAGAAACTGCCCTTCTTAT GGAAAAGAATAAACAGGAGAAGGAACTTTGGGACAAGCAGCAGAAGGAACAGAAAGAGAAGCTGAGAAGG GAGGAACAAGAGCGCAAAGCTGGAAAGACACAGGATGCAGATGAACGTGACTCCACTGAGAATCAGCACA AAGCAAAGGATGGACAAGAGAAAAAAGACAGCAAACAGACCAAGACGGAAGACAGAGAGCTTTCAGCAGA CGGGGCCCAGGAAGCCACAGGAACACAAAGACAAAGTAAGAGTGAGCATGAAGCTTCTGATGCTAAGGTA CCTGTGGAAGGTAAAAGGTGTCCCACGTCAGAGGCGCAGAAGAGGCCAGCAGATGTGTCCCCTGCATCCG TGTCAGGAGAGCTGAATGCAGGCAAGGCTCAGCGAGAACCTTTGACGAGAGCACGAAGTGAAGAAATGGG GAGAATTGTGCCGGGACTGCCTTTGGGCTGGGCCAAGTTTCTTGATCCAATCACCGGGACCTTTCGTTAC TACCATTCCCCCACAAACACAGTTCATATGTATCCACCTGAAATGGCTCCTTCGTCTGCACCTCCTTCCA CCCCGCCCACTCACAAAGTCAAGCCCCAGGTCCCTGCCGAGCGGGACAGGGAGCCATCGAAACTGAAGCG CTCCTACTCCTCACCAGATATCACTCAGGCCCTGCAGGAGGAGGAGAAGAGGAGGCCGGCAGTGACCCCG ATGGTCAACCGGGAAAACAAGCCACCATGTTACCCTAAAGCTGAGATTTCGAGGCTTTCTGCTTCTCAGA TTCGGAACCTCAATCCTGTATTTGGAGGATCAGGACCAGCTCTTACTGGACTTCGTAATTTGGGAAATAC TTGTTACATGAACTCAATCTTGCAGTGCCTGTGTAATGCTCCACATCTGGCTGATTATTTCAACCGAAAC TGCTACCAGGATGATATCAACAGGTCAAATTTGTTGGGGCATAAAGGTGAAGTGGCAGAAGAATTTGGTA TAATCATGAAAGCACTGTGGACAGGACAGTATAGATACATCAGTCCAAAGGACTTTAAAGTCACCATTGG TAAGATTAATGACCAGTTTGCAGGCTCCAGCCAACAGGATTCACAAGAGCTGCTTCTGTTCCTCATGGAT GGCCTGCATGAGGATCTGAATAAGGCTGACAATCGGAAGAGGCACAAGGAAGAGAACAACGAGCACCTGG ATGACCTGCAGGCGGCCGAGCACGCCTGGCAGAAGCACAAGCAGCTCAACGAGTCCATCATCGTGGCCCT GTTCCAGGGCCAGTTCAAGTCCACAGTGCAGTGCCTCACCTGCCGCAGGAGGTCGCGCACCTTCGAGGCC TTCATGTACTTGTCTTTGCCGCTAGCATCCACAAGTAAATGTACTCTACAGGACTGCCTTAGATTATTTT CCAAAGAAGAAAAGCTTACAGATAACAACAGATTTTACTGCAGCCATTGCCGAGCTCGGCGGGATTCTCT AAAGAAAATAGAAATCTGGAAATTACCTCCTGTGCTGTTAGTGCACCTGAAACGATTTTCCTATGACGGC AGGTGGAAGCAGAAGCTGCAAACATCCGTGGATTTCCCATTGGAAAATCTTGACCTGTCACAGTACGTTA TTGGTCCAAAAAACAGCTTGAAGAAATATAACTTGTTTTCTGTTTCAAACCACTACGGCGGGCTAGACGG AGGCCACTACACAGCCTACTGTAAGAACGCGGCAAGGCAGCGCTGGTTTAAGTTTGATGACCATGAAGTT TCTGATATCTCTGTGTCTTCTGTGAGGTCATCAGCAGCTTATATCCTCTTTTATACTTCCCTGGGACCAC GCATAACTGATGTAGCCACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211601 representing NM_019729
Red=Cloning site Green=Tags(s) MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNYIHSAQKIFKTAEECRLDRDEERAYVLYMKYVAVY NLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQE MGREDSGAAAKRSVENLLDSKTKTQRINGEKSEGAAAAERGAITAKELYTMMMDKNTSLIIMDARKIQDY QHSCILDSLSVPEEAISPGVTASWIEANLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRSLKDA LFKWESKTVLRHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRAEEVSVSLDFTYPSLEEPVPSKLPTQ MPPPPIETNEKALLVTDQDEKLRLSTQPALAGPGAAPRAEASPIIQPAPATKSVPQVDRTKKPSVKLPED HRIKSENTDQSGRVLSDRSTKPVFPSPTTMLTDEEKARIHQETALLMEKNKQEKELWDKQQKEQKEKLRR EEQERKAGKTQDADERDSTENQHKAKDGQEKKDSKQTKTEDRELSADGAQEATGTQRQSKSEHEASDAKV PVEGKRCPTSEAQKRPADVSPASVSGELNAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDPITGTFRY YHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDREPSKLKRSYSSPDITQALQEEEKRRPAVTP MVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRN CYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMD GLHEDLNKADNRKRHKEENNEHLDDLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCRRRSRTFEA FMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDG RWKQKLQTSVDFPLENLDLSQYVIGPKNSLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEV SDISVSSVRSSAAYILFYTSLGPRITDVAT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019729 |
| ORF Size | 3240 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019729.3, NP_062703.2 |
| RefSeq Size | 4177 bp |
| RefSeq ORF | 3243 bp |
| Locus ID | 84092 |
| UniProt ID | Q80U87 |
| Gene Summary | Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1 (By similarity). Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223467 | Usp8 (untagged) - Mouse ubiquitin specific peptidase 8 (Usp8), (10ug) |
CNY 7,528.00 |
|
| MR211601 | Usp8 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 8 (Usp8) |
CNY 7,528.00 |
|
| MR211601L3 | Lenti ORF clone of Usp8 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 8 (Usp8) |
CNY 10,260.00 |
|
| MR211601L4 | Lenti ORF clone of Usp8 (mGFP-tagged) - Mouse ubiquitin specific peptidase 8 (Usp8) |
CNY 10,260.00 |
