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Rhbdf1 (NM_010117) Mouse Tagged ORF Clone
CAT＃: MG210964
- TrueORF®
Rhbdf1 (tGFP-tagged) - Mouse rhomboid family 1 (Drosophila) (Rhbdf1)
ORF Plasmid: DDK
"NM_010117" in other vectors (4)
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CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C16ORF8; Dist; Dist1; Egfr-rs; mKIAA4242 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210964 representing NM_010117
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAAGCCAGGAGAGACAGTACTAGCAGCCTGCAACGCAAGAAACCCCCATGGCTCAAGTTGGACA TCCCAGCCGTGGTGCCCCCAGCAGCAGAGGAGCCCAGCTTCCTGCAGCCCGTGCGGCGACAGGCTTTCTT GCGGAGTGTGAGTATGCCAGCTGAGACAGCCCGGGTCCCATCACCCCACCATGAGCCTCGCCGGCTGGTG CTGCAGCGTCAGACATCCATCACACAGACCATCCGCAGGGGGACAGCAGACTGGTTTGGAGTGAGCAAGG ACAGTGACAGCACCCAGAAGTGGCAGCGCAAGAGCATCCGTCACTGCAGCCAGCGGTATGGGAAGCTAAA GCCGCAGGTCATCCGGGAGCTGGACCTGCCCAGCCAGGACAATGTGTCCCTAACCAGCACCGAGACACCA CCTCCCCTCTACGTGGGGCCATGCCAGCTGGGCATGCAGAAGATCATAGACCCTCTGGCCCGAGGCCGGG CCTTTCGCATGGCTGATGACACTGCCGATGGCCTGAGTGCCCCCCACACTCCCGTCACACCAGGTGCAGC CTCCCTCTGCTCTTTCTCCAGCTCCCGCTCAGGTTTCAACCGACTCCCTCGGCGTCGCAAACGTGAATCG GTGGCCAAGATGAGCTTCCGGGCCGCTGCAGCATTGGTGAAGGGTCGGTCTATTAGGGATGGCACTTTAC GAAGGGGACAGCGCCGAAGCTTTACCCCTGCCAGCTTTCTTGAGGAAGACATGGTGGACTTCCCTGATGA GTTGGATACATCTTTCTTTGCTCGGGAAGGTGTCCTCCACGAGGAGATGTCCACGTACCCAGATGAGGTG TTTGAGTCCCCATCAGAGGCAGCGCTCAAGGACTGGGAGAAAGCCCCGGATCAGGCCGACCTCACGGGTG GGGCCCTGGACCGCAGTGAGCTTGAGAGAAGCCACTTGATGTTGCCCCTGGAACGAGGCTGGAGAAAGCA GAAGGAAGGCGGCCCTCTAGCCCCGCAGCCCAAGGTGCGGCTTCGCCAGGAGGTGGTCAGCGCGGCGGGA CCCAGGAGGGGTCAGCGTATCGCTGTGCCAGTGCGCAAACTCTTCGCTCGGGAAAAGCGGCCTTATGGTC TGGGGATGGTGGGACGGCTCACCAACCGCACGTACCGGAAACGCATCGACAGCTATGTCAAGCGGCAGAT CGAGGACATGGATGATCACAGGCCCTTCTTCACTTACTGGCTCACCTTTGTGCACTCACTGGTCACCATT CTGGCTGTGTGCATCTATGGCATAGCGCCCGTGGGCTTCTCGCAGCATGAGACGGTGGACTCGGTACTTC GGAAGCGGGGTGTCTATGAGAATGTCAAATACGTCCAGCAGGAGAACTTCTGGATTGGCCCCAGCTCGGA GGCCCTCATTCACTTGGGTGCCAAGTTCTCACCCTGCATGCGTCAGGACCCACAGGTGCACAGCTTCATC CTGGCTGCCCGGGAGCGTGAAAAGCACTCGGCTTGCTGTGTACGCAATGACCGGTCTGGGTGTGTGCAGA CTTCCAAGGAGGAGTGCTCGTCCACGTTGGCGGTGTGGGTGAAGTGGCCCGTTCATCCTAGTGCCCCAGA CCTTGCAGGCAACAAGAGACAGTTTGGTTCTGTCTGCCACCAGGACCCCAGGGTATGTGACGAGCCTTCC TCTGAGGATCCCCACGAGTGGCCAGAAGATATCACCAAGTGGCCGATCTGCACCAAAAGCAGCGCGGGGA ACCACACTAACCACCCTCACATGGACTGTGTCATAACAGGCCGGCCTTGCTGCATTGGCACCAAGGGCAG GTGTGAGATCACCTCCCGGGAGTACTGTGACTTCATGAGGGGCTACTTCCATGAGGAGGCCACACTCTGC TCTCAGGTGCACTGCATGGACGATGTCTGTGGCCTCCTGCCTTTCCTCAATCCTGAGGTGCCTGACCAGT TCTACCGCCTGTGGCTGTCCCTCTTCCTGCACGCTGGGATCCTGCACTGCTTGGTGTCCATCTGCTTCCA GATGACGGTCCTGCGGGACCTGGAGAAGCTGGCAGGCTGGCACCGCATAGCCATCATCTACCTGCTGAGT GGTATCACGGGCAATCTAGCCAGTGCCATCTTCCTGCCATACCGGGCAGAGGTAGGTCCGGCCGGCTCCC AGTTTGGCATCCTGGCCTGCCTCTTTGTGGAGTTGTTCCAGAGCTGGCAGATCCTGGCCCGACCCTGGCG TGCCTTCTTCAAGCTCCTGGCCGTGGTGCTCTTCCTCTTTGCCTTTGGGCTGCTGCCCTGGATCGACAAC TTCGCCCACATCTCGGGCTTTGTCAGCGGCCTCTTCCTTTCCTTTGCCTTCCTGCCTTACATCAGCTTTG GCAAGTTCGACCTGTACCGCAAGCGCTGCCAGATCATCATCTTCCAGGTCGTCTTCCTGGGCCTGCTGGC CGGCCTGGTGGTCCTCTTCTACTTCTACCCGGTGCGCTGTGAATGGTGCGAGTTCCTCACCTGCATCCCT TTCACGGACAAGTTCTGTGAAAAGTACGAGCTGGACGCTCAGCTCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210964 representing NM_010117
Red=Cloning site Green=Tags(s) MSEARRDSTSSLQRKKPPWLKLDIPAVVPPAAEEPSFLQPVRRQAFLRSVSMPAETARVPSPHHEPRRLV LQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETP PPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRES VAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEV FESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAG PRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTI LAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFI LAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPS SEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLC SQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLS GITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPWIDN FAHISGFVSGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYFYPVRCEWCEFLTCIP FTDKFCEKYELDAQLH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010117 |
| ORF Size | 2568 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010117.1, NP_034247.1 |
| RefSeq Size | 2904 bp |
| RefSeq ORF | 2571 bp |
| Locus ID | 13650 |
| UniProt ID | Q6PIX5 |
| Gene Summary | Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205414 | Rhbdf1 (untagged) - Mouse rhomboid family 1 (Drosophila) (Rhbdf1), (10ug) |
CNY 2,000.00 |
|
| MR210964 | Rhbdf1 (Myc-DDK-tagged) - Mouse rhomboid family 1 (Drosophila) (Rhbdf1) |
CNY 6,280.00 |
|
| MR210964L3 | Lenti ORF clone of Rhbdf1 (Myc-DDK-tagged) - Mouse rhomboid family 1 (Drosophila) (Rhbdf1) |
CNY 9,410.00 |
|
| MR210964L4 | Lenti ORF clone of Rhbdf1 (mGFP-tagged) - Mouse rhomboid family 1 (Drosophila) (Rhbdf1) |
CNY 9,410.00 |
