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Tlr4 (NM_021297) Mouse Tagged ORF Clone
CAT＃: MG210887
- TrueORF®
Tlr4 (tGFP-tagged) - Mouse toll-like receptor 4 (Tlr4)
ORF Plasmid: DDK
"NM_021297" in other vectors (4)
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CNY 7,720.00
CNY 8,080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Lps; Ly87; Ran/M1; Ras; Rasl2-8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210887 representing NM_021297
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCCTCCCTGGCTCCTGGCTAGGACTCTGATCATGGCACTGTTCTTCTCCTGCCTGACACCGGGAA GCTTGAATCCCTGCATAGAGGTAGTTCCTAATATTACCTACCAATGCATGGATCAGAAACTCAGCAAAGT CCCTGATGACATTCCTTCTTCAACCAAGAACATAGATCTGAGCTTCAACCCCTTGAAGATCTTAAAAAGC TATAGCTTCTCCAATTTTTCAGAACTTCAGTGGCTGGATTTATCCAGGTGTGAAATTGAAACAATTGAAG ACAAGGCATGGCATGGCTTACACCACCTCTCAAACTTGATACTGACAGGAAACCCTATCCAGAGTTTTTC CCCAGGAAGTTTCTCTGGACTAACAAGTTTAGAGAATCTGGTGGCTGTGGAGACAAAATTGGCCTCTCTA GAAAGCTTCCCTATTGGACAGCTTATAACCTTAAAGAAACTCAATGTGGCTCACAATTTTATACATTCCT GTAAGTTACCTGCATATTTTTCCAATCTGACGAACCTAGTACATGTGGATCTTTCTTATAACTATATTCA AACTATTACTGTCAATGACTTACAGTTTCTACGTGAAAATCCACAAGTCAATCTCTCTTTAGACATATCT TTGAACCCAATTGACTTCATTCAAGACCAAGCCTTTCAGGGAATTAAGCTCCATGAACTGACTCTAAGAG GTAATTTTAATAGCTCAAATATAATGAAAACTTGCCTTCAAAACCTGGCTGGTTTACACGTCCATCGGTT GATCTTGGGAGAATTTAAAGATGAAAGGAATCTGGAAATTTTTGAACCCTCTATCATGGAAGGACTATGT GATGTGACCATTGATGAGTTCAGGTTAACACATACAAATGATTTTTCAGATGATATTGTTAAGTTCCATT GCTTGGCGAATGTTTCTGCAATGTCTCTGGCAGGTGTATCTATAAAATATCTAGAAGATGTTCCTAAACA TTTCAAATGGCAATCCTTATCAATCATTAGATGTCAACTTAAGCAGTTTCCAACTCTGGATCTACCCTTT CTTAAAAGTTTGACTTTAACTATGAACAAAGGGTCTATCAGTTTTAAAAAAGTGGCCCTGCCAAGTCTCA GCTATCTAGATCTTAGTAGAAATGCACTGAGCTTTAGTGGTTGCTGTTCTTATTCTGATTTGGGAACAAA CAGCCTGAGACACTTAGACCTCAGCTTCAATGGTGCCATCATTATGAGTGCCAATTTCATGGGTCTAGAA GAGCTGCAGCACCTGGATTTTCAGCACTCTACTTTAAAAAGGGTCACAGAATTCTCAGCGTTCTTATCCC TTGAAAAGCTACTTTACCTTGACATCTCTTATACTAACACCAAAATTGACTTCGATGGTATATTTCTTGG CTTGACCAGTCTCAACACATTAAAAATGGCTGGCAATTCTTTCAAAGACAACACCCTTTCAAATGTCTTT GCAAACACAACAAACTTGACATTCCTGGATCTTTCTAAATGTCAATTGGAACAAATATCTTGGGGGGTAT TTGACACCCTCCATAGACTTCAATTATTAAATATGAGTCACAACAATCTATTGTTTTTGGATTCATCCCA TTATAACCAGCTGTATTCCCTCAGCACTCTTGATTGCAGTTTCAATCGCATAGAGACATCTAAAGGAATA CTGCAACATTTTCCAAAGAGTCTAGCCTTCTTCAATCTTACTAATAATTCTGTTGCTTGTATATGTGAAC ATCAGAAATTCCTGCAGTGGGTCAAGGACCAGAAGCAGTTCTTGGTGAATGTTGAACAAATGACATGTGC AACACCTGTAGAGATGAATACCTCCTTAGTGTTGGATTTTAATAATTCTACCTGTTATATGTACAAGACA ATCATCAGTGTGTCAGTGATCAGTGTGATTGTGGTATCCACTGTAGCATTTCTGATATACCACTTCTATT TTCACCTGATACTTATTGCTGGCTGTAAAAAGTACAGCAGAGGAGAAAGCATCTATGATGCATTTGTGAT CTACTCGAGTCAGAATGAGGACTGGGTGAGAAATGAGCTAGTAAAGAATTTAGAAGAAGGAGTGCCCCGC TTTCACCTCTGCCTTCACTACAGAGACTTTATTCCTGGTGTAGCCATTGCTGCCAACATCATCCAGGAAG GCTTCCACAAGAGCCGGAAGGTTATTGTGGTAGTGTCTAGACACTTTATTCAGAGCCGTTGGTGTATCTT TGAATATGAGATTGCTCAAACATGGCAGTTTCTGAGCAGCCGCTCTGGCATCATCTTCATTGTCCTTGAG AAGGTTGAGAAGTCCCTGCTGAGGCAGCAGGTGGAATTGTATCGCCTTCTTAGCAGAAACACCTACCTGG AATGGGAGGACAATCCTCTGGGGAGGCACATCTTCTGGAGAAGACTTAAAAAGGCCCTATTGGATGGAAA AGCCTCGAATCCTGAGCAAACAGCAGAGGAAGAACAAGAAACGGCAACTTGGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210887 representing NM_021297
Red=Cloning site Green=Tags(s) MMPPWLLARTLIMALFFSCLTPGSLNPCIEVVPNITYQCMDQKLSKVPDDIPSSTKNIDLSFNPLKILKS YSFSNFSELQWLDLSRCEIETIEDKAWHGLHHLSNLILTGNPIQSFSPGSFSGLTSLENLVAVETKLASL ESFPIGQLITLKKLNVAHNFIHSCKLPAYFSNLTNLVHVDLSYNYIQTITVNDLQFLRENPQVNLSLDIS LNPIDFIQDQAFQGIKLHELTLRGNFNSSNIMKTCLQNLAGLHVHRLILGEFKDERNLEIFEPSIMEGLC DVTIDEFRLTHTNDFSDDIVKFHCLANVSAMSLAGVSIKYLEDVPKHFKWQSLSIIRCQLKQFPTLDLPF LKSLTLTMNKGSISFKKVALPSLSYLDLSRNALSFSGCCSYSDLGTNSLRHLDLSFNGAIIMSANFMGLE ELQHLDFQHSTLKRVTEFSAFLSLEKLLYLDISYTNTKIDFDGIFLGLTSLNTLKMAGNSFKDNTLSNVF ANTTNLTFLDLSKCQLEQISWGVFDTLHRLQLLNMSHNNLLFLDSSHYNQLYSLSTLDCSFNRIETSKGI LQHFPKSLAFFNLTNNSVACICEHQKFLQWVKDQKQFLVNVEQMTCATPVEMNTSLVLDFNNSTCYMYKT IISVSVISVIVVSTVAFLIYHFYFHLILIAGCKKYSRGESIYDAFVIYSSQNEDWVRNELVKNLEEGVPR FHLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSRHFIQSRWCIFEYEIAQTWQFLSSRSGIIFIVLE KVEKSLLRQQVELYRLLSRNTYLEWEDNPLGRHIFWRRLKKALLDGKASNPEQTAEEEQETATWT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021297 |
| ORF Size | 2505 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021297.2, NP_067272.1 |
| RefSeq Size | 3847 bp |
| RefSeq ORF | 2508 bp |
| Locus ID | 21898 |
| UniProt ID | Q9QUK6 |
| Gene Summary | This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203163 | Tlr4 (untagged) - Mouse toll-like receptor 4 (Tlr4), (10ug) |
CNY 2,000.00 |
|
| MR210887 | Tlr4 (Myc-DDK-tagged) - Mouse toll-like receptor 4 (Tlr4) |
CNY 6,120.00 |
|
| MR210887L3 | Lenti ORF clone of Tlr4 (Myc-DDK-tagged) - Mouse toll-like receptor 4 (Tlr4) |
CNY 9,220.00 |
|
| MR210887L4 | Lenti ORF clone of Tlr4 (mGFP-tagged) - Mouse toll-like receptor 4 (Tlr4) |
CNY 8,520.00 |
