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Jup (NM_010593) Mouse Tagged ORF Clone
CAT＃: MG210428
- TrueORF®
Jup (tGFP-tagged) - Mouse junction plakoglobin (Jup)
ORF Plasmid: DDK
"NM_010593" in other vectors (4)
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CNY 8,170.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ctnng; D930025P04Rik; PG |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210428 representing NM_010593
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGATGAACCTTATTGAGCAGCCCATCAAGGTGACAGAGTGGCAACAGACATACACCTACGACT CGGGCATCCACTCCGGCGTCAATACCTGTGTGCCCTCTGTAAGCAGCAAGGGCATCATGGATGAGGATGA TGCCTGCGGCAGACAGTACACACTCAAGAAGACTACCACCTATACACAAGGGGTGCCACAGAACCAAGGT GACCTGGAATACCAGATGTCCACAACGGCCAGAGCCAAGCGGGTGCGGGAGGCCATGTGTCCAGGGGTCT CAGGCGAGGACAGTTCTCTACTGCTGGCCACCCAGGTGGAGGGGCAGACAACCAATCTGCAGCGCCTGGC CGAACCATCCCAGTTGCTCAAGTCGGCCATCGTCCATCTCATCAACTACCAGGATGATGCAGAGCTGGCC ACCCGGGCTCTGCCTGAGCTCACCAAGCTGCTCAACGATGAGGACCCGGTAGTGGTGACCAAGGCGGCTA TGATCGTGAACCAGCTGTCCAAGAAGGAGGCATCCCGCCGGGCTCTAATGGGCTCGCCCCAGCTGGTGGC GGCGGTGGTGCGGACTATGCAGAACACCAGTGACCTGGATACTGCCCGGTGTACCACCAGCATCCTGCAC AACCTCTCTCACCACCGAGAGGGCTTGCTCGCCATATTCAAGTCTGGAGGCATCCCTGCCCTAGTCCGAA TGCTCAGTTCCCCCGTAGAATCTGTCCTGTTCTACGCCATCACCACACTGCACAACCTGCTGCTCTATCA GGAGGGCGCCAAGATGGCGGTGCGTCTGGCAGACGGGCTGCAGAAGATGGTGCCCCTACTCAACAAGAAC AACCCTAAGTTCCTGGCCATCACTACTGACTGCCTGCAGCTCCTGGCCTATGGCAACCAGGAGAGCAAGC TGATCATCCTGGCCAATGGGGGACCCCAAGGGCTTGTGCAGATCATGAGGAACTACAGCTACGAGAAGCT TCTGTGGACCACCAGCCGTGTGCTCAAGGTGCTCTCCGTGTGTCCTAGCAACAAGCCCGCCATCGTGGAG GCTGGTGGGATGCAGGCTCTGGGCAAGCACTTGACAAGCAACAGTCCCCGCCTGGTGCAGAACTGCCTGT GGACTCTGCGCAATCTCTCGGATGTAGCCACCAAGCAGGAGGGCCTGGAGAGTGTGCTGAAGATCCTGGT CAACCAACTGAGTGTAGATGACGTCAACGTCCTCACCTGTGCCACGGGCACCCTCTCCAACCTGACCTGC AACAACAGCAAAAACAAGACGCTGGTGACACAGAACAGTGGCGTGGAGGCGCTCATCCACGCCATCCTGA GAGCCGGAGACAAAGATGACATCACAGAGCCCGCTGTCTGTGCCCTGCGCCACCTTACCAGCCGTCACCC CGAGGCTGAGATGGCCCAGAACTCTGTGCGCCTCAACTATGGAATCCCGGCCATTGTGAAACTGCTCAAC CAGCCAAACCAGTGGCCACTGGTCAAGGCAACTATTGGCCTGATCCGGAACCTGGCTCTGTGCCCAGCCA ACCATGCCCCTCTGCAGGAGGCCGCGGTCATTCCCCGCCTCGTCCAGCTGTTGGTCAAGGCCCACCAGGA CGCCCAGCGCCATGTGGCTGCAGGCACCCAGCAGCCCTACACGGATGGTGTGAGGATGGAGGAGATCGTG GAAGGCTGCACCGGAGCCCTGCACATCCTCGCCCGGGATCCCATGAACCGCATGGAGATCTTCCGGCTCA ACACCATTCCCCTGTTTGTCCAGCTCCTGTACTCCTCTGTGGAGAACATCCAGCGTGTGGCTGCGGGAGT GCTCTGCGAGCTGGCCCAGGACAAGGAGGCTGCCGACGCCATTGATGCGGAGGGCGCCTCTGCCCCTCTC ATGGAGCTACTCCATTCCCGCAATGAGGGCACCGCGACCTACGCTGCTGCTGTCCTGTTCCGCATCTCTG AGGACAAGAACCCAGATTACCGTAAGCGAGTGTCTGTGGAGCTCACCAATTCCCTCTTCAAGCATGACCC AGCTGCCTGGGAGGCTGCCCAGAGTATGATCCCGATCAACGAACCCTATGCAGACGACATGGATGCCACC TACCGCCCCATGTACTCCAGCGATGTGCCTCTGGACCCTCTGGACATGCACATGGACCTGGATGGAGACT ACCCCATGGACACCTACAGTGACGGCCTGAGGCCTCCCTACCCCACTGCAGACCACATGCTGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210428 representing NM_010593
Red=Cloning site Green=Tags(s) MEVMNLIEQPIKVTEWQQTYTYDSGIHSGVNTCVPSVSSKGIMDEDDACGRQYTLKKTTTYTQGVPQNQG DLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQTTNLQRLAEPSQLLKSAIVHLINYQDDAELA TRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILH NLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKN NPKFLAITTDCLQLLAYGNQESKLIILANGGPQGLVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVE AGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLESVLKILVNQLSVDDVNVLTCATGTLSNLTC NNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLN QPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIV EGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPL MELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYADDMDAT YRPMYSSDVPLDPLDMHMDLDGDYPMDTYSDGLRPPYPTADHMLA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010593 |
| ORF Size | 2235 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010593.2, NP_034723.1 |
| RefSeq Size | 5009 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 16480 |
| UniProt ID | Q02257 |
| Gene Summary | Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221283 | Jup (untagged) - Mouse junction plakoglobin (Jup), (10ug) |
CNY 9,690.00 |
|
| MR210428 | Jup (Myc-DDK-tagged) - Mouse junction plakoglobin (Jup) |
CNY 7,410.00 |
|
| MR210428L3 | Lenti ORF clone of Jup (Myc-DDK-tagged) - Mouse junction plakoglobin (Jup) |
CNY 9,310.00 |
|
| MR210428L4 | Lenti ORF clone of Jup (mGFP-tagged) - Mouse junction plakoglobin (Jup) |
CNY 9,310.00 |
